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Article: Development of monoclonal antibody-based galactomannoprotein antigen-capture ELISAs to detect Aspergillus fumigatus infection in the invasive aspergillosis rabbit models

TitleDevelopment of monoclonal antibody-based galactomannoprotein antigen-capture ELISAs to detect Aspergillus fumigatus infection in the invasive aspergillosis rabbit models
Authors
Issue Date2012
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/10096/index.htm
Citation
European Journal Of Clinical Microbiology And Infectious Diseases, 2012, v. 31 n. 11, p. 2943-2950 How to Cite?
AbstractAspergillus fumigatus is one of the most prominent opportunistic fungal pathogens in immunocompromised hosts. Early recognition of this infection along with prompt antifungal therapy may increase the survival rate. We expressed two potential bio-markers of A. fumigatus infection-galactomannoprotein Afmp1p and Afmp4p in Pichia pastoris. We generated 33 monoclonal antibodies (MAbs), 20 against recombinant Afmp1p (rAfmp1p) and the other 13 against recombinant Afmp4p (rAfmp4p). Subsequently, we developed two antigen-capture enzyme-linked immunosorbent assays (ELISAs) which employed MAbs as both the capture and the detection antibodies for rAfmp1p and rAfmp4p. The two antigen-capture ELISAs specifically detected Afmp1p/Afmp4p in cultures of A. fumigatus and had no cross-reaction with other tested pathogenic fungi, including Penicillium marneffei and other pathogenic Aspergillus species. The Afmp1p-captured ELISA would be positive even when the culture supernatant of A. fumigatus had been diluted to 128-fold of its original concentration. The two antigen ELISAs could capture circulating or excreted antigens during the acute phase of invasive aspergillosis (IA) in the animal model, and had no cross-reactivity to other Aspergillus-challenged animal models. We developed two antigen-capture ELISAs for the laboratory diagnosis of A. fumigatus infection. These two antigen-capture ELISAs may be useful in the clinical diagnosis of aspergillosis. © 2012 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/157704
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.173
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, ZYen_US
dc.contributor.authorCai, JPen_US
dc.contributor.authorQiu, LWen_US
dc.contributor.authorHao, Wen_US
dc.contributor.authorPan, YXen_US
dc.contributor.authorTung, ETKen_US
dc.contributor.authorLau, CCYen_US
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorLau, SKPen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorChe, XYen_US
dc.date.accessioned2012-08-08T08:52:25Z-
dc.date.available2012-08-08T08:52:25Z-
dc.date.issued2012en_US
dc.identifier.citationEuropean Journal Of Clinical Microbiology And Infectious Diseases, 2012, v. 31 n. 11, p. 2943-2950en_US
dc.identifier.issn0934-9723en_US
dc.identifier.urihttp://hdl.handle.net/10722/157704-
dc.description.abstractAspergillus fumigatus is one of the most prominent opportunistic fungal pathogens in immunocompromised hosts. Early recognition of this infection along with prompt antifungal therapy may increase the survival rate. We expressed two potential bio-markers of A. fumigatus infection-galactomannoprotein Afmp1p and Afmp4p in Pichia pastoris. We generated 33 monoclonal antibodies (MAbs), 20 against recombinant Afmp1p (rAfmp1p) and the other 13 against recombinant Afmp4p (rAfmp4p). Subsequently, we developed two antigen-capture enzyme-linked immunosorbent assays (ELISAs) which employed MAbs as both the capture and the detection antibodies for rAfmp1p and rAfmp4p. The two antigen-capture ELISAs specifically detected Afmp1p/Afmp4p in cultures of A. fumigatus and had no cross-reaction with other tested pathogenic fungi, including Penicillium marneffei and other pathogenic Aspergillus species. The Afmp1p-captured ELISA would be positive even when the culture supernatant of A. fumigatus had been diluted to 128-fold of its original concentration. The two antigen ELISAs could capture circulating or excreted antigens during the acute phase of invasive aspergillosis (IA) in the animal model, and had no cross-reactivity to other Aspergillus-challenged animal models. We developed two antigen-capture ELISAs for the laboratory diagnosis of A. fumigatus infection. These two antigen-capture ELISAs may be useful in the clinical diagnosis of aspergillosis. © 2012 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/10096/index.htmen_US
dc.relation.ispartofEuropean Journal of Clinical Microbiology and Infectious Diseasesen_US
dc.titleDevelopment of monoclonal antibody-based galactomannoprotein antigen-capture ELISAs to detect Aspergillus fumigatus infection in the invasive aspergillosis rabbit modelsen_US
dc.typeArticleen_US
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_US
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s10096-012-1645-3en_US
dc.identifier.pmid22669560-
dc.identifier.scopuseid_2-s2.0-84867980812en_US
dc.identifier.hkuros216415-
dc.identifier.spage2943en_US
dc.identifier.epage2950en_US
dc.identifier.isiWOS:000310247800011-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridWang, ZY=55236932600en_US
dc.identifier.scopusauthoridCai, JP=35557916700en_US
dc.identifier.scopusauthoridQiu, LW=55236564500en_US
dc.identifier.scopusauthoridHao, W=55236330400en_US
dc.identifier.scopusauthoridPan, YX=35765899800en_US
dc.identifier.scopusauthoridTung, ETK=23398349800en_US
dc.identifier.scopusauthoridLau, CCY=8398162900en_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridChe, XY=7005743182en_US
dc.identifier.citeulike10776382-

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