File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Genomic characterization of seven distinct bat coronaviruses in Kenya

TitleGenomic characterization of seven distinct bat coronaviruses in Kenya
Authors
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusres
Citation
Virus Research, 2012, v. 167 n. 1, p. 67-73 How to Cite?
AbstractTo better understand the genetic diversity and genomic features of 41 coronaviruses (CoVs) identified from Kenya bats in 2006, seven CoVs as representatives of seven different phylogenetic groups identified from partial polymerase gene sequences, were subjected to extensive genomic sequencing. As a result, 15-16kb nucleotide sequences encoding complete RNA dependent RNA polymerase, spike, envelope, membrane, and nucleocapsid proteins plus other open reading frames (ORFs) were generated. Sequences analysis confirmed that the CoVs from Kenya bats are divergent members of Alphacoronavirus and Betacoronavirus genera. Furthermore, the CoVs BtKY22, BtKY41, and BtKY43 in Alphacoronavirus genus and BtKY24 in Betacoronavirus genus are likely representatives of 4 novel CoV species. BtKY27 and BtKY33 are members of the established bat CoV species in Alphacoronavirus genus and BtKY06 is a member of the established bat CoV species in Betacoronavirus genus. The genome organization of these seven CoVs is similar to other known CoVs from the same groups except for differences in the number of putative ORFs following the N gene. The present results confirm a significant diversity of CoVs circulating in Kenya bats. These Kenya bat CoVs are phylogenetically distant from any previously described human and animal CoVs. However, because of the examples of host switching among CoVs after relatively minor sequence changes in S1 domain of spike protein, a further surveillance in animal reservoirs and understanding the interface between host susceptibility is critical for predicting and preventing the potential threat of bat CoVs to public health.
Persistent Identifierhttp://hdl.handle.net/10722/157700
ISSN
2015 Impact Factor: 2.526
2015 SCImago Journal Rankings: 1.259
ISI Accession Number ID
Funding AgencyGrant Number
CDC (Atlanta, GA)
Funding Information:

We thank Ivan Kuzmin, Michael Niezgoda, and Charles E. Rupprecht from Division of High Consequence Pathogens and Pathology, CDC, Atlanta, GA; Robert F. Breiman from Global Disease Detection Division, CDC-Kenya, Nairobi, Kenya; and Bernard Agwanda from National Museum, Kenya Wildlife Service, Nairobi, Kenya for excellent technical and logistical assistance and field study. The study was supported in part by the Global Disease Detection program of CDC (Atlanta, GA).

References

 

DC FieldValueLanguage
dc.contributor.authorTao, Yen_US
dc.contributor.authorTang, Ken_US
dc.contributor.authorShi, Men_US
dc.contributor.authorConrardy, Cen_US
dc.contributor.authorLi, KSMen_US
dc.contributor.authorLau, SKPen_US
dc.contributor.authorAnderson, LJen_US
dc.contributor.authorTong, Sen_US
dc.date.accessioned2012-08-08T08:52:24Z-
dc.date.available2012-08-08T08:52:24Z-
dc.date.issued2012en_US
dc.identifier.citationVirus Research, 2012, v. 167 n. 1, p. 67-73en_US
dc.identifier.issn0168-1702en_US
dc.identifier.urihttp://hdl.handle.net/10722/157700-
dc.description.abstractTo better understand the genetic diversity and genomic features of 41 coronaviruses (CoVs) identified from Kenya bats in 2006, seven CoVs as representatives of seven different phylogenetic groups identified from partial polymerase gene sequences, were subjected to extensive genomic sequencing. As a result, 15-16kb nucleotide sequences encoding complete RNA dependent RNA polymerase, spike, envelope, membrane, and nucleocapsid proteins plus other open reading frames (ORFs) were generated. Sequences analysis confirmed that the CoVs from Kenya bats are divergent members of Alphacoronavirus and Betacoronavirus genera. Furthermore, the CoVs BtKY22, BtKY41, and BtKY43 in Alphacoronavirus genus and BtKY24 in Betacoronavirus genus are likely representatives of 4 novel CoV species. BtKY27 and BtKY33 are members of the established bat CoV species in Alphacoronavirus genus and BtKY06 is a member of the established bat CoV species in Betacoronavirus genus. The genome organization of these seven CoVs is similar to other known CoVs from the same groups except for differences in the number of putative ORFs following the N gene. The present results confirm a significant diversity of CoVs circulating in Kenya bats. These Kenya bat CoVs are phylogenetically distant from any previously described human and animal CoVs. However, because of the examples of host switching among CoVs after relatively minor sequence changes in S1 domain of spike protein, a further surveillance in animal reservoirs and understanding the interface between host susceptibility is critical for predicting and preventing the potential threat of bat CoVs to public health.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusresen_US
dc.relation.ispartofVirus Researchen_US
dc.subject.meshAmino Acid Sequence-
dc.subject.meshChiroptera - virology-
dc.subject.meshCoronavirus - chemistry - classification - genetics - isolation and purification-
dc.subject.meshCoronavirus Infections - veterinary - virology-
dc.subject.meshGenome, Viral-
dc.titleGenomic characterization of seven distinct bat coronaviruses in Kenyaen_US
dc.typeArticleen_US
dc.identifier.emailLi, KSM: kenn105@hkucc.hku.hken_US
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailTong, S: sot1@cdc.gov-
dc.identifier.authorityLau, SKP=rp00486en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.virusres.2012.04.007en_US
dc.identifier.pmid22561208-
dc.identifier.scopuseid_2-s2.0-84861481480en_US
dc.identifier.hkuros209481-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861481480&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume167en_US
dc.identifier.issue1en_US
dc.identifier.spage67en_US
dc.identifier.epage73en_US
dc.identifier.isiWOS:000305496700009-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridTong, S=7201486834en_US
dc.identifier.scopusauthoridAnderson, LJ=7403741945en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US
dc.identifier.scopusauthoridLi, KSM=24759122500en_US
dc.identifier.scopusauthoridConrardy, C=26429167000en_US
dc.identifier.scopusauthoridShi, M=25951822000en_US
dc.identifier.scopusauthoridTang, K=55092278600en_US
dc.identifier.scopusauthoridTao, Y=36744564300en_US
dc.identifier.citeulike10622812-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats