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Article: Isolation and characterization of a novel Betacoronavirus subgroup a coronavirus, rabbit coronavirus HKU14, from domestic rabbits

TitleIsolation and characterization of a novel Betacoronavirus subgroup a coronavirus, rabbit coronavirus HKU14, from domestic rabbits
Authors
Issue Date2012
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2012, v. 86 n. 10, p. 5481-5496 How to Cite?
AbstractWe describe the isolation and characterization of a novel Betacoronavirus subgroup A coronavirus, rabbit coronavirus HKU14 (RbCoV HKU14), from domestic rabbits. The virus was detected in 11 (8.1%) of 136 rabbit fecal samples by reverse transcriptase PCR (RT-PCR), with a viral load of up to 108 copies/ml. RbCoV HKU14 was able to replicate in HRT-18G and RK13 cells with cytopathic effects. Northern blotting confirmed the production of subgenomic mRNAs coding for the HE, S, NS5a, E, M, and N proteins. Subgenomic mRNA analysis revealed a transcription regulatory sequence, 5′-UCUAAAC-3′. Phylogenetic analysis showed that RbCoV HKU14 formed a distinct branch among Betacoronavirus subgroup A coronaviruses, being most closely related to but separate from the species Betacoronavirus 1. A comparison of the conserved replicase domains showed that Rb-CoV HKU14 possessed <90% amino acid identities to most members of Betacoronavirus 1 in ADP-ribose 1"-phosphatase (ADRP) and nidoviral uridylate-specific endoribonuclease (NendoU), indicating that RbCoV HKU14 should represent a separate species. RbCoV HKU14 also possessed genomic features distinct from those of other Betacoronavirus subgroup A coronaviruses, including a unique NS2a region with a variable number of small open reading frames (ORFs). Recombination analysis revealed possible recombination events during the evolution of RbCoV HKU14 and members of Betacoronavirus 1, which may have occurred during cross-species transmission. Molecular clock analysis using RNA-dependent RNA polymerase (RdRp) genes dated the most recent common ancestor of RbCoV HKU14 to around 2002, suggesting that this virus has emerged relatively recently. Antibody against RbCoV was detected in 20 (67%) of 30 rabbit sera tested by an N-protein-based Western blot assay, whereas neutralizing antibody was detected in 1 of these 20 rabbits. © 2012, American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/157698
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYip, CCYen_HK
dc.contributor.authorFan, RYYen_HK
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorGuo, Ren_HK
dc.contributor.authorLam, CSFen_HK
dc.contributor.authorTsang, AKLen_HK
dc.contributor.authorLai, KKYen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorChe, XYen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2012-08-08T08:52:23Z-
dc.date.available2012-08-08T08:52:23Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Virology, 2012, v. 86 n. 10, p. 5481-5496en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/157698-
dc.description.abstractWe describe the isolation and characterization of a novel Betacoronavirus subgroup A coronavirus, rabbit coronavirus HKU14 (RbCoV HKU14), from domestic rabbits. The virus was detected in 11 (8.1%) of 136 rabbit fecal samples by reverse transcriptase PCR (RT-PCR), with a viral load of up to 108 copies/ml. RbCoV HKU14 was able to replicate in HRT-18G and RK13 cells with cytopathic effects. Northern blotting confirmed the production of subgenomic mRNAs coding for the HE, S, NS5a, E, M, and N proteins. Subgenomic mRNA analysis revealed a transcription regulatory sequence, 5′-UCUAAAC-3′. Phylogenetic analysis showed that RbCoV HKU14 formed a distinct branch among Betacoronavirus subgroup A coronaviruses, being most closely related to but separate from the species Betacoronavirus 1. A comparison of the conserved replicase domains showed that Rb-CoV HKU14 possessed <90% amino acid identities to most members of Betacoronavirus 1 in ADP-ribose 1"-phosphatase (ADRP) and nidoviral uridylate-specific endoribonuclease (NendoU), indicating that RbCoV HKU14 should represent a separate species. RbCoV HKU14 also possessed genomic features distinct from those of other Betacoronavirus subgroup A coronaviruses, including a unique NS2a region with a variable number of small open reading frames (ORFs). Recombination analysis revealed possible recombination events during the evolution of RbCoV HKU14 and members of Betacoronavirus 1, which may have occurred during cross-species transmission. Molecular clock analysis using RNA-dependent RNA polymerase (RdRp) genes dated the most recent common ancestor of RbCoV HKU14 to around 2002, suggesting that this virus has emerged relatively recently. Antibody against RbCoV was detected in 20 (67%) of 30 rabbit sera tested by an N-protein-based Western blot assay, whereas neutralizing antibody was detected in 1 of these 20 rabbits. © 2012, American Society for Microbiology.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.-
dc.rightsCopyright © American Society for Microbiology, [Journal of Virology, 2012, v. 86 n. 10, p. 5481-5496]-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals, Domestic - virology-
dc.subject.meshCell Line-
dc.subject.meshCoronaviridae - chemistry - classification - genetics - isolation and purification-
dc.subject.meshRabbits - virology-
dc.titleIsolation and characterization of a novel Betacoronavirus subgroup a coronavirus, rabbit coronavirus HKU14, from domestic rabbitsen_HK
dc.typeArticleen_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailYip, CCY: yipcyril@hku.hken_HK
dc.identifier.emailZheng, BJ: bzheng@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYip, CCY=rp01721en_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/JVI.06927-11en_HK
dc.identifier.pmid22398294-
dc.identifier.pmcidPMC3347282-
dc.identifier.scopuseid_2-s2.0-84861313379en_HK
dc.identifier.hkuros204330-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861313379&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume86en_HK
dc.identifier.issue10en_HK
dc.identifier.spage5481en_HK
dc.identifier.epage5496en_HK
dc.identifier.eissn1098-5514-
dc.identifier.isiWOS:000303787100006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, PCY=55177222700en_HK
dc.identifier.scopusauthoridYip, CCY=14016999800en_HK
dc.identifier.scopusauthoridFan, RYY=15519269300en_HK
dc.identifier.scopusauthoridHuang, Y=36007277800en_HK
dc.identifier.scopusauthoridWang, M=53265130500en_HK
dc.identifier.scopusauthoridGuo, R=15839273700en_HK
dc.identifier.scopusauthoridLam, CSF=25950267400en_HK
dc.identifier.scopusauthoridTsang, AKL=7006979247en_HK
dc.identifier.scopusauthoridLai, KKY=36708103200en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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