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Article: Predominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008
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TitlePredominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008
 
AuthorsHo, PL1
Yeung, MK1
Lo, WU1
Tse, H1
Li, Z1
Lai, ELY
Chow, KH1
To, KK1
Yam, WC1
 
KeywordsAntimicrobial Drug Resistance
CTX-M-14 Beta-Lactamase
Enterobacteriaceae
Plasmids
Restriction Fragment Length Polymorphism
 
Issue Date2012
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio
 
CitationDiagnostic Microbiology and Infectious Disease, 2012, v. 73 n. 2, p. 182-186 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.diagmicrobio.2012.03.009
 
AbstractThis study assessed the temporal changes in the molecular epidemiology of bacteremic Escherichia coli isolates producing CTX-M-14 in Hong Kong. Blood isolates from 1996 to 1998 (period 1, n = 50) and 2007 to 2008 (period 2, n = 117) were investigated by molecular methods. CTX-M-type ESBL was carried by 98.2% (164/167) of the isolates. In both periods, the CTX-M-9 group and CTX-M-14 allele were the predominant ESBL type. The major clones were found to change from ST68 and ST405 in period 1 to ST131, ST69, and ST12 in period 2. Among 65 CTX-M-14-producing plasmids investigated further, 54 had the FII replicon. Replicon sequence typing and plasmid polymerase chain reaction-restriction fragment length polymorphism showed that 79.6% (43/54) of the FII plasmid subset was similar to the completely sequenced plasmid, pHK01 (human urine, Hong Kong, 2004). These pHK01-like plasmids were found to have spread to the major clones (ST68, ST405, and ST131) and multiple singleton isolates of all 4 phylogenetic groups. © 2012 Elsevier Inc.
 
ISSN0732-8893
2013 Impact Factor: 2.568
2013 SCImago Journal Rankings: 1.275
 
DOIhttp://dx.doi.org/10.1016/j.diagmicrobio.2012.03.009
 
ISI Accession Number IDWOS:000305102100014
Funding AgencyGrant Number
Health, Welfare and Food Bureau of the Government of the HKSAR
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Government of the HKSAR and from the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHo, PL
 
dc.contributor.authorYeung, MK
 
dc.contributor.authorLo, WU
 
dc.contributor.authorTse, H
 
dc.contributor.authorLi, Z
 
dc.contributor.authorLai, ELY
 
dc.contributor.authorChow, KH
 
dc.contributor.authorTo, KK
 
dc.contributor.authorYam, WC
 
dc.date.accessioned2012-08-08T08:52:20Z
 
dc.date.available2012-08-08T08:52:20Z
 
dc.date.issued2012
 
dc.description.abstractThis study assessed the temporal changes in the molecular epidemiology of bacteremic Escherichia coli isolates producing CTX-M-14 in Hong Kong. Blood isolates from 1996 to 1998 (period 1, n = 50) and 2007 to 2008 (period 2, n = 117) were investigated by molecular methods. CTX-M-type ESBL was carried by 98.2% (164/167) of the isolates. In both periods, the CTX-M-9 group and CTX-M-14 allele were the predominant ESBL type. The major clones were found to change from ST68 and ST405 in period 1 to ST131, ST69, and ST12 in period 2. Among 65 CTX-M-14-producing plasmids investigated further, 54 had the FII replicon. Replicon sequence typing and plasmid polymerase chain reaction-restriction fragment length polymorphism showed that 79.6% (43/54) of the FII plasmid subset was similar to the completely sequenced plasmid, pHK01 (human urine, Hong Kong, 2004). These pHK01-like plasmids were found to have spread to the major clones (ST68, ST405, and ST131) and multiple singleton isolates of all 4 phylogenetic groups. © 2012 Elsevier Inc.
 
dc.description.naturepostprint
 
dc.identifier.citationDiagnostic Microbiology and Infectious Disease, 2012, v. 73 n. 2, p. 182-186 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.diagmicrobio.2012.03.009
 
dc.identifier.citeulike10604397
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.diagmicrobio.2012.03.009
 
dc.identifier.epage186
 
dc.identifier.hkuros204614
 
dc.identifier.hkuros225983
 
dc.identifier.isiWOS:000305102100014
Funding AgencyGrant Number
Health, Welfare and Food Bureau of the Government of the HKSAR
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Government of the HKSAR and from the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

 
dc.identifier.issn0732-8893
2013 Impact Factor: 2.568
2013 SCImago Journal Rankings: 1.275
 
dc.identifier.issue2
 
dc.identifier.pmid22521053
 
dc.identifier.scopuseid_2-s2.0-84861183904
 
dc.identifier.spage182
 
dc.identifier.urihttp://hdl.handle.net/10722/157695
 
dc.identifier.volume73
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio
 
dc.publisher.placeUnited States
 
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
 
dc.relation.referencesReferences in Scopus
 
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Diagnostic Microbiology and Infectious Disease. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Diagnostic Microbiology and Infectious Disease, 2012, v. 73 n. 2, p. 182-186. DOI: 10.1016/j.diagmicrobio.2012.03.009
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectAntimicrobial Drug Resistance
 
dc.subjectCTX-M-14 Beta-Lactamase
 
dc.subjectEnterobacteriaceae
 
dc.subjectPlasmids
 
dc.subjectRestriction Fragment Length Polymorphism
 
dc.titlePredominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong