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Article: Predominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008

TitlePredominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008
Authors
KeywordsAntimicrobial Drug Resistance
CTX-M-14 Beta-Lactamase
Enterobacteriaceae
Plasmids
Restriction Fragment Length Polymorphism
Issue Date2012
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio
Citation
Diagnostic Microbiology and Infectious Disease, 2012, v. 73 n. 2, p. 182-186 How to Cite?
Abstract
This study assessed the temporal changes in the molecular epidemiology of bacteremic Escherichia coli isolates producing CTX-M-14 in Hong Kong. Blood isolates from 1996 to 1998 (period 1, n = 50) and 2007 to 2008 (period 2, n = 117) were investigated by molecular methods. CTX-M-type ESBL was carried by 98.2% (164/167) of the isolates. In both periods, the CTX-M-9 group and CTX-M-14 allele were the predominant ESBL type. The major clones were found to change from ST68 and ST405 in period 1 to ST131, ST69, and ST12 in period 2. Among 65 CTX-M-14-producing plasmids investigated further, 54 had the FII replicon. Replicon sequence typing and plasmid polymerase chain reaction-restriction fragment length polymorphism showed that 79.6% (43/54) of the FII plasmid subset was similar to the completely sequenced plasmid, pHK01 (human urine, Hong Kong, 2004). These pHK01-like plasmids were found to have spread to the major clones (ST68, ST405, and ST131) and multiple singleton isolates of all 4 phylogenetic groups. © 2012 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/157695
ISSN
2013 Impact Factor: 2.568
2013 SCImago Journal Rankings: 1.275
ISI Accession Number ID
Funding AgencyGrant Number
Health, Welfare and Food Bureau of the Government of the HKSAR
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Government of the HKSAR and from the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorHo, PLen_US
dc.contributor.authorYeung, MKen_US
dc.contributor.authorLo, WUen_US
dc.contributor.authorTse, Hen_US
dc.contributor.authorLi, Zen_US
dc.contributor.authorLai, ELYen_US
dc.contributor.authorChow, KHen_US
dc.contributor.authorTo, KKen_US
dc.contributor.authorYam, WCen_US
dc.date.accessioned2012-08-08T08:52:20Z-
dc.date.available2012-08-08T08:52:20Z-
dc.date.issued2012en_US
dc.identifier.citationDiagnostic Microbiology and Infectious Disease, 2012, v. 73 n. 2, p. 182-186en_US
dc.identifier.issn0732-8893en_US
dc.identifier.urihttp://hdl.handle.net/10722/157695-
dc.description.abstractThis study assessed the temporal changes in the molecular epidemiology of bacteremic Escherichia coli isolates producing CTX-M-14 in Hong Kong. Blood isolates from 1996 to 1998 (period 1, n = 50) and 2007 to 2008 (period 2, n = 117) were investigated by molecular methods. CTX-M-type ESBL was carried by 98.2% (164/167) of the isolates. In both periods, the CTX-M-9 group and CTX-M-14 allele were the predominant ESBL type. The major clones were found to change from ST68 and ST405 in period 1 to ST131, ST69, and ST12 in period 2. Among 65 CTX-M-14-producing plasmids investigated further, 54 had the FII replicon. Replicon sequence typing and plasmid polymerase chain reaction-restriction fragment length polymorphism showed that 79.6% (43/54) of the FII plasmid subset was similar to the completely sequenced plasmid, pHK01 (human urine, Hong Kong, 2004). These pHK01-like plasmids were found to have spread to the major clones (ST68, ST405, and ST131) and multiple singleton isolates of all 4 phylogenetic groups. © 2012 Elsevier Inc.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobioen_US
dc.relation.ispartofDiagnostic Microbiology and Infectious Diseaseen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Diagnostic Microbiology and Infectious Disease. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Diagnostic Microbiology and Infectious Disease, 2012, v. 73 n. 2, p. 182-186. DOI: 10.1016/j.diagmicrobio.2012.03.009-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAntimicrobial Drug Resistanceen_US
dc.subjectCTX-M-14 Beta-Lactamaseen_US
dc.subjectEnterobacteriaceaeen_US
dc.subjectPlasmidsen_US
dc.subjectRestriction Fragment Length Polymorphismen_US
dc.titlePredominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008en_US
dc.typeArticleen_US
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_US
dc.identifier.emailLo, WU: stephlo@hku.hk-
dc.identifier.emailTse, H: herman@graduate.hku.hk-
dc.identifier.emailLai, ELY: elylai@hku.hk-
dc.identifier.emailChow, KH: khchowb@hku.hk-
dc.identifier.emailTo, KKW: kelvinto@hkucc.hku.hk-
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hk-
dc.identifier.authorityHo, PL=rp00406en_US
dc.description.naturepostprinten_US
dc.identifier.doi10.1016/j.diagmicrobio.2012.03.009en_US
dc.identifier.pmid22521053-
dc.identifier.scopuseid_2-s2.0-84861183904en_US
dc.identifier.hkuros204614-
dc.identifier.hkuros225983-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861183904&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume73en_US
dc.identifier.issue2en_US
dc.identifier.spage182en_US
dc.identifier.epage186en_US
dc.identifier.isiWOS:000305102100014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridYeung, MK=37040000300en_US
dc.identifier.scopusauthoridLo, WU=35558916700en_US
dc.identifier.scopusauthoridTse, H=55183843500en_US
dc.identifier.scopusauthoridLi, Z=55039481400en_US
dc.identifier.scopusauthoridLai, EL=8238477100en_US
dc.identifier.scopusauthoridChow, KH=55188615700en_US
dc.identifier.scopusauthoridTo, KK=55024912100en_US
dc.identifier.scopusauthoridYam, WC=55038322100en_US
dc.identifier.citeulike10604397-
dc.customcontrol.immutablesml 130529-

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