Article: Feline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats

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TitleFeline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats
AuthorsWoo, PCY
Lau, SKP3
Wong, BHL3
Fan, RYY3
Wong, AYP3
Zhang, AJX
Wu, Y3
Choi, GKY3
Li, KSM3
Hui, J2
Wang, M
Zheng, BJ
Chan, KH
Yuen, KY3
Issue Date2012
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
CitationProceedings of The National Academy of Sciences of The United States of America, 2012, v. 109 n. 14, p. 5435-5440 [How to Cite?]
DOI: http://dx.doi.org/10.1073/pnas.1119972109
AbstractWe describe the discovery and isolation of a paramyxovirus, feline morbillivirus (FmoPV), from domestic cat (Felis catus). FmoPV RNA was detected in 56 (12.3%) of 457 stray cats (53 urine, four rectal swabs, and one blood sample) by RT-PCR. Complete genome sequencing of three FmoPV strains showed genome sizes of 16,050 bases, the largest among morbilliviruses, because of unusually long 5′ trailer sequences of 400 nt. FmoPV possesses identical gene contents (3′-N-P/V/C-M-F-H-L-5′) and is phylogenetically clustered with other morbilliviruses. IgG against FmoPV N protein was positive in 49 sera (76.7%) of 56 RT-PCR-positive cats, but 78 (19.4%) of 401 RT-PCR-negative cats (P < 0.0001) byWestern blot. FmoPV was isolated from CRFK feline kidney cells, causing cytopathic effects with cell rounding, detachment, lysis, and syncytia formation. FmoPV could also replicate in subsequent passages in primate Vero E6 cells. Infected cell lines exhibited finely granular and diffuse cytoplasmic fluorescence on immunostaining for FmoPV N protein. Electron microscopy showed enveloped virus with typical "herringbone" appearance of helical N in paramyxoviruses. Histological examination of necropsy tissues in two FmoPV-positive cats revealed interstitial inflammatory infiltrate and tubular degeneration/necrosis in kidneys, with decreased cauxin expression in degenerated tubular epithelial cells, compatible with tubulointerstitial nephritis (TIN). Immunohistochemical staining revealed FmoPV N protein-positive renal tubular cells and mononuclear cells in lymph nodes. A case-control study showed the presence of TIN in seven of 12 cats with FmoPV infection, but only two of 15 cats without FmoPV infection (P < 0.05), suggesting an association between FmoPV and TIN.
ISSN0027-8424
2011 Impact Factor: 9.681
2011 SCImago Journal Rankings: 1.754
DOIhttp://dx.doi.org/10.1073/pnas.1119972109
ISI Accession Number IDWOS:000302294700066
Funding AgencyGrant Number
Research Grants CouncilHKU 783611M
University of Hong Kong
Providence Foundation Limited
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health
Funding Information:

We thank Director Alan Chi-Kong Wong, Siu-Fai Leung, Thomas Hon-Chung Sit, and Howard Kai-Hay Wong [Agriculture Fisheries and Conservation Department (AFCD)]; Veterinary Officers of the AFCD Animal Management Centres for assistance and collection of samples; Dr. Chi-Kwan Yip, Dr. Cassius Chan, and Dr. King-Shun Lo for animal necropsy; Prof. Yamashita (Iwate University) for providing the anti-cat cauxin antibody; and Prof. K. C. Chan for the MAC387 antibody. We thank Mrs. Carol Yu, Prof. Richard Yu, Mr. Hui Hoy, and Mr. Hui Ming for support in genomic sequencing platform, and Ms. Eunice Lam for her donation for emerging infectious disease research. This work was supported in part by Research Grants Council Grant HKU 783611M; the Strategic Research Theme Fund and University Development Fund of the University of Hong Kong; the Providence Foundation Limited in memory of the late Dr. Lui Hac Minh; and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health.

PubMed Central IDPMC3325679
ReferencesReferences in Scopus
GrantsMolecular evolution and interspecies jumping in picornaviruses
DC Field
Value
dc.contributor.authorWoo, PCY
dc.contributor.authorLau, SKP
dc.contributor.authorWong, BHL
dc.contributor.authorFan, RYY
dc.contributor.authorWong, AYP
dc.contributor.authorZhang, AJX
dc.contributor.authorWu, Y
dc.contributor.authorChoi, GKY
dc.contributor.authorLi, KSM
dc.contributor.authorHui, J
dc.contributor.authorWang, M
dc.contributor.authorZheng, BJ
dc.contributor.authorChan, KH
dc.contributor.authorYuen, KY
dc.date.accessioned2012-08-08T08:52:15Z
dc.date.available2012-08-08T08:52:15Z
dc.date.issued2012
dc.description.abstractWe describe the discovery and isolation of a paramyxovirus, feline morbillivirus (FmoPV), from domestic cat (Felis catus). FmoPV RNA was detected in 56 (12.3%) of 457 stray cats (53 urine, four rectal swabs, and one blood sample) by RT-PCR. Complete genome sequencing of three FmoPV strains showed genome sizes of 16,050 bases, the largest among morbilliviruses, because of unusually long 5′ trailer sequences of 400 nt. FmoPV possesses identical gene contents (3′-N-P/V/C-M-F-H-L-5′) and is phylogenetically clustered with other morbilliviruses. IgG against FmoPV N protein was positive in 49 sera (76.7%) of 56 RT-PCR-positive cats, but 78 (19.4%) of 401 RT-PCR-negative cats (P < 0.0001) byWestern blot. FmoPV was isolated from CRFK feline kidney cells, causing cytopathic effects with cell rounding, detachment, lysis, and syncytia formation. FmoPV could also replicate in subsequent passages in primate Vero E6 cells. Infected cell lines exhibited finely granular and diffuse cytoplasmic fluorescence on immunostaining for FmoPV N protein. Electron microscopy showed enveloped virus with typical "herringbone" appearance of helical N in paramyxoviruses. Histological examination of necropsy tissues in two FmoPV-positive cats revealed interstitial inflammatory infiltrate and tubular degeneration/necrosis in kidneys, with decreased cauxin expression in degenerated tubular epithelial cells, compatible with tubulointerstitial nephritis (TIN). Immunohistochemical staining revealed FmoPV N protein-positive renal tubular cells and mononuclear cells in lymph nodes. A case-control study showed the presence of TIN in seven of 12 cats with FmoPV infection, but only two of 15 cats without FmoPV infection (P < 0.05), suggesting an association between FmoPV and TIN.
dc.description.grantMolecular evolution and interspecies jumping in picornaviruses
dc.description.grantcode105985
dc.description.natureLink_to_OA_fulltext
dc.identifier.citationProceedings of The National Academy of Sciences of The United States of America, 2012, v. 109 n. 14, p. 5435-5440 [How to Cite?]
DOI: http://dx.doi.org/10.1073/pnas.1119972109
dc.identifier.doihttp://dx.doi.org/10.1073/pnas.1119972109
dc.identifier.epage5440
dc.identifier.hkuros204318
dc.identifier.isiWOS:000302294700066
Funding AgencyGrant Number
Research Grants CouncilHKU 783611M
University of Hong Kong
Providence Foundation Limited
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health
Funding Information:

We thank Director Alan Chi-Kong Wong, Siu-Fai Leung, Thomas Hon-Chung Sit, and Howard Kai-Hay Wong [Agriculture Fisheries and Conservation Department (AFCD)]; Veterinary Officers of the AFCD Animal Management Centres for assistance and collection of samples; Dr. Chi-Kwan Yip, Dr. Cassius Chan, and Dr. King-Shun Lo for animal necropsy; Prof. Yamashita (Iwate University) for providing the anti-cat cauxin antibody; and Prof. K. C. Chan for the MAC387 antibody. We thank Mrs. Carol Yu, Prof. Richard Yu, Mr. Hui Hoy, and Mr. Hui Ming for support in genomic sequencing platform, and Ms. Eunice Lam for her donation for emerging infectious disease research. This work was supported in part by Research Grants Council Grant HKU 783611M; the Strategic Research Theme Fund and University Development Fund of the University of Hong Kong; the Providence Foundation Limited in memory of the late Dr. Lui Hac Minh; and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health.

dc.identifier.issn0027-8424
2011 Impact Factor: 9.681
2011 SCImago Journal Rankings: 1.754
dc.identifier.issue14
dc.identifier.pmcidPMC3325679
dc.identifier.pmid22431644
dc.identifier.scopuseid_2-s2.0-84859478260
dc.identifier.spage5435
dc.identifier.urihttp://hdl.handle.net/10722/157686
dc.identifier.volume109
dc.languageeng
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
dc.publisher.placeUnited States
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshAnimals, Domestic
dc.subject.meshBlotting, Western
dc.subject.meshCats
dc.subject.meshCell Line
dc.subject.meshImmunohistochemistry
dc.subject.meshMicroscopy, Electron
dc.subject.meshMorbillivirus - Pathogenicity
dc.subject.meshNephritis, Interstitial - Virology
dc.subject.meshPhylogeny
dc.subject.meshPolymerase Chain Reaction
dc.titleFeline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats
dc.typeArticle
Author Affiliations
  1. Guangdong Center for Disease Control and Prevention
  2. PathLab. Medical Laboratories
  3. The University of Hong Kong