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Article: Feline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats

TitleFeline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats
Authors
Issue Date2012
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings of The National Academy of Sciences of The United States of America, 2012, v. 109 n. 14, p. 5435-5440 How to Cite?
Abstract
We describe the discovery and isolation of a paramyxovirus, feline morbillivirus (FmoPV), from domestic cat (Felis catus). FmoPV RNA was detected in 56 (12.3%) of 457 stray cats (53 urine, four rectal swabs, and one blood sample) by RT-PCR. Complete genome sequencing of three FmoPV strains showed genome sizes of 16,050 bases, the largest among morbilliviruses, because of unusually long 5′ trailer sequences of 400 nt. FmoPV possesses identical gene contents (3′-N-P/V/C-M-F-H-L-5′) and is phylogenetically clustered with other morbilliviruses. IgG against FmoPV N protein was positive in 49 sera (76.7%) of 56 RT-PCR-positive cats, but 78 (19.4%) of 401 RT-PCR-negative cats (P < 0.0001) byWestern blot. FmoPV was isolated from CRFK feline kidney cells, causing cytopathic effects with cell rounding, detachment, lysis, and syncytia formation. FmoPV could also replicate in subsequent passages in primate Vero E6 cells. Infected cell lines exhibited finely granular and diffuse cytoplasmic fluorescence on immunostaining for FmoPV N protein. Electron microscopy showed enveloped virus with typical "herringbone" appearance of helical N in paramyxoviruses. Histological examination of necropsy tissues in two FmoPV-positive cats revealed interstitial inflammatory infiltrate and tubular degeneration/necrosis in kidneys, with decreased cauxin expression in degenerated tubular epithelial cells, compatible with tubulointerstitial nephritis (TIN). Immunohistochemical staining revealed FmoPV N protein-positive renal tubular cells and mononuclear cells in lymph nodes. A case-control study showed the presence of TIN in seven of 12 cats with FmoPV infection, but only two of 15 cats without FmoPV infection (P < 0.05), suggesting an association between FmoPV and TIN.
Persistent Identifierhttp://hdl.handle.net/10722/157686
ISSN
2013 Impact Factor: 9.809
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants CouncilHKU 783611M
University of Hong Kong
Providence Foundation Limited
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health
Funding Information:

We thank Director Alan Chi-Kong Wong, Siu-Fai Leung, Thomas Hon-Chung Sit, and Howard Kai-Hay Wong [Agriculture Fisheries and Conservation Department (AFCD)]; Veterinary Officers of the AFCD Animal Management Centres for assistance and collection of samples; Dr. Chi-Kwan Yip, Dr. Cassius Chan, and Dr. King-Shun Lo for animal necropsy; Prof. Yamashita (Iwate University) for providing the anti-cat cauxin antibody; and Prof. K. C. Chan for the MAC387 antibody. We thank Mrs. Carol Yu, Prof. Richard Yu, Mr. Hui Hoy, and Mr. Hui Ming for support in genomic sequencing platform, and Ms. Eunice Lam for her donation for emerging infectious disease research. This work was supported in part by Research Grants Council Grant HKU 783611M; the Strategic Research Theme Fund and University Development Fund of the University of Hong Kong; the Providence Foundation Limited in memory of the late Dr. Lui Hac Minh; and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health.

References
Grants

 

Author Affiliations
  1. Guangdong Center for Disease Control and Prevention
  2. The University of Hong Kong
  3. PathLab. Medical Laboratories
DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorLau, SKPen_US
dc.contributor.authorWong, BHLen_US
dc.contributor.authorFan, RYYen_US
dc.contributor.authorWong, AYPen_US
dc.contributor.authorZhang, AJXen_US
dc.contributor.authorWu, Yen_US
dc.contributor.authorChoi, GKYen_US
dc.contributor.authorLi, KSMen_US
dc.contributor.authorHui, Jen_US
dc.contributor.authorWang, Men_US
dc.contributor.authorZheng, BJen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:52:15Z-
dc.date.available2012-08-08T08:52:15Z-
dc.date.issued2012en_US
dc.identifier.citationProceedings of The National Academy of Sciences of The United States of America, 2012, v. 109 n. 14, p. 5435-5440en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/157686-
dc.description.abstractWe describe the discovery and isolation of a paramyxovirus, feline morbillivirus (FmoPV), from domestic cat (Felis catus). FmoPV RNA was detected in 56 (12.3%) of 457 stray cats (53 urine, four rectal swabs, and one blood sample) by RT-PCR. Complete genome sequencing of three FmoPV strains showed genome sizes of 16,050 bases, the largest among morbilliviruses, because of unusually long 5′ trailer sequences of 400 nt. FmoPV possesses identical gene contents (3′-N-P/V/C-M-F-H-L-5′) and is phylogenetically clustered with other morbilliviruses. IgG against FmoPV N protein was positive in 49 sera (76.7%) of 56 RT-PCR-positive cats, but 78 (19.4%) of 401 RT-PCR-negative cats (P < 0.0001) byWestern blot. FmoPV was isolated from CRFK feline kidney cells, causing cytopathic effects with cell rounding, detachment, lysis, and syncytia formation. FmoPV could also replicate in subsequent passages in primate Vero E6 cells. Infected cell lines exhibited finely granular and diffuse cytoplasmic fluorescence on immunostaining for FmoPV N protein. Electron microscopy showed enveloped virus with typical "herringbone" appearance of helical N in paramyxoviruses. Histological examination of necropsy tissues in two FmoPV-positive cats revealed interstitial inflammatory infiltrate and tubular degeneration/necrosis in kidneys, with decreased cauxin expression in degenerated tubular epithelial cells, compatible with tubulointerstitial nephritis (TIN). Immunohistochemical staining revealed FmoPV N protein-positive renal tubular cells and mononuclear cells in lymph nodes. A case-control study showed the presence of TIN in seven of 12 cats with FmoPV infection, but only two of 15 cats without FmoPV infection (P < 0.05), suggesting an association between FmoPV and TIN.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Domesticen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCatsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMorbillivirus - Pathogenicityen_US
dc.subject.meshNephritis, Interstitial - Virologyen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.titleFeline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic catsen_US
dc.typeArticleen_US
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1073/pnas.1119972109en_US
dc.identifier.pmid22431644en_US
dc.identifier.pmcidPMC3325679-
dc.identifier.scopuseid_2-s2.0-84859478260en_US
dc.identifier.hkuros204318-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84859478260&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume109en_US
dc.identifier.issue14en_US
dc.identifier.spage5435en_US
dc.identifier.epage5440en_US
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000302294700066-
dc.publisher.placeUnited Statesen_US
dc.relation.projectMolecular evolution and interspecies jumping in picornaviruses-
dc.identifier.scopusauthoridWoo, PCY=55177222700en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US
dc.identifier.scopusauthoridWong, BHL=7402023413en_US
dc.identifier.scopusauthoridFan, RYY=15519269300en_US
dc.identifier.scopusauthoridWong, AYP=53464494100en_US
dc.identifier.scopusauthoridZhang, AJX=37008823400en_US
dc.identifier.scopusauthoridWu, Y=54898602600en_US
dc.identifier.scopusauthoridChoi, GKY=35423450200en_US
dc.identifier.scopusauthoridLi, KSM=24759122500en_US
dc.identifier.scopusauthoridHui, J=50961254900en_US
dc.identifier.scopusauthoridWang, M=55018738000en_US
dc.identifier.scopusauthoridZheng, BJ=54935584800en_US
dc.identifier.scopusauthoridChan, KH=7406034307en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.customcontrol.immutablejt 130405-

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