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Article: Feline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats
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TitleFeline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats
 
AuthorsWoo, PCY2
Lau, SKP2
Wong, BHL2
Fan, RYY2
Wong, AYP2
Zhang, AJX2
Wu, Y2
Choi, GKY2
Li, KSM2
Hui, J3
Wang, M1
Zheng, BJ2
Chan, KH2
Yuen, KY2
 
Issue Date2012
 
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
 
CitationProceedings of The National Academy of Sciences of The United States of America, 2012, v. 109 n. 14, p. 5435-5440 [How to Cite?]
DOI: http://dx.doi.org/10.1073/pnas.1119972109
 
AbstractWe describe the discovery and isolation of a paramyxovirus, feline morbillivirus (FmoPV), from domestic cat (Felis catus). FmoPV RNA was detected in 56 (12.3%) of 457 stray cats (53 urine, four rectal swabs, and one blood sample) by RT-PCR. Complete genome sequencing of three FmoPV strains showed genome sizes of 16,050 bases, the largest among morbilliviruses, because of unusually long 5′ trailer sequences of 400 nt. FmoPV possesses identical gene contents (3′-N-P/V/C-M-F-H-L-5′) and is phylogenetically clustered with other morbilliviruses. IgG against FmoPV N protein was positive in 49 sera (76.7%) of 56 RT-PCR-positive cats, but 78 (19.4%) of 401 RT-PCR-negative cats (P < 0.0001) byWestern blot. FmoPV was isolated from CRFK feline kidney cells, causing cytopathic effects with cell rounding, detachment, lysis, and syncytia formation. FmoPV could also replicate in subsequent passages in primate Vero E6 cells. Infected cell lines exhibited finely granular and diffuse cytoplasmic fluorescence on immunostaining for FmoPV N protein. Electron microscopy showed enveloped virus with typical "herringbone" appearance of helical N in paramyxoviruses. Histological examination of necropsy tissues in two FmoPV-positive cats revealed interstitial inflammatory infiltrate and tubular degeneration/necrosis in kidneys, with decreased cauxin expression in degenerated tubular epithelial cells, compatible with tubulointerstitial nephritis (TIN). Immunohistochemical staining revealed FmoPV N protein-positive renal tubular cells and mononuclear cells in lymph nodes. A case-control study showed the presence of TIN in seven of 12 cats with FmoPV infection, but only two of 15 cats without FmoPV infection (P < 0.05), suggesting an association between FmoPV and TIN.
 
ISSN0027-8424
2012 Impact Factor: 9.737
2012 SCImago Journal Rankings: 5.473
 
DOIhttp://dx.doi.org/10.1073/pnas.1119972109
 
PubMed Central IDPMC3325679
 
ISI Accession Number IDWOS:000302294700066
Funding AgencyGrant Number
Research Grants CouncilHKU 783611M
University of Hong Kong
Providence Foundation Limited
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health
Funding Information:

We thank Director Alan Chi-Kong Wong, Siu-Fai Leung, Thomas Hon-Chung Sit, and Howard Kai-Hay Wong [Agriculture Fisheries and Conservation Department (AFCD)]; Veterinary Officers of the AFCD Animal Management Centres for assistance and collection of samples; Dr. Chi-Kwan Yip, Dr. Cassius Chan, and Dr. King-Shun Lo for animal necropsy; Prof. Yamashita (Iwate University) for providing the anti-cat cauxin antibody; and Prof. K. C. Chan for the MAC387 antibody. We thank Mrs. Carol Yu, Prof. Richard Yu, Mr. Hui Hoy, and Mr. Hui Ming for support in genomic sequencing platform, and Ms. Eunice Lam for her donation for emerging infectious disease research. This work was supported in part by Research Grants Council Grant HKU 783611M; the Strategic Research Theme Fund and University Development Fund of the University of Hong Kong; the Providence Foundation Limited in memory of the late Dr. Lui Hac Minh; and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health.

 
ReferencesReferences in Scopus
 
GrantsMolecular evolution and interspecies jumping in picornaviruses
 
DC FieldValue
dc.contributor.authorWoo, PCY
 
dc.contributor.authorLau, SKP
 
dc.contributor.authorWong, BHL
 
dc.contributor.authorFan, RYY
 
dc.contributor.authorWong, AYP
 
dc.contributor.authorZhang, AJX
 
dc.contributor.authorWu, Y
 
dc.contributor.authorChoi, GKY
 
dc.contributor.authorLi, KSM
 
dc.contributor.authorHui, J
 
dc.contributor.authorWang, M
 
dc.contributor.authorZheng, BJ
 
dc.contributor.authorChan, KH
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2012-08-08T08:52:15Z
 
dc.date.available2012-08-08T08:52:15Z
 
dc.date.issued2012
 
dc.description.abstractWe describe the discovery and isolation of a paramyxovirus, feline morbillivirus (FmoPV), from domestic cat (Felis catus). FmoPV RNA was detected in 56 (12.3%) of 457 stray cats (53 urine, four rectal swabs, and one blood sample) by RT-PCR. Complete genome sequencing of three FmoPV strains showed genome sizes of 16,050 bases, the largest among morbilliviruses, because of unusually long 5′ trailer sequences of 400 nt. FmoPV possesses identical gene contents (3′-N-P/V/C-M-F-H-L-5′) and is phylogenetically clustered with other morbilliviruses. IgG against FmoPV N protein was positive in 49 sera (76.7%) of 56 RT-PCR-positive cats, but 78 (19.4%) of 401 RT-PCR-negative cats (P < 0.0001) byWestern blot. FmoPV was isolated from CRFK feline kidney cells, causing cytopathic effects with cell rounding, detachment, lysis, and syncytia formation. FmoPV could also replicate in subsequent passages in primate Vero E6 cells. Infected cell lines exhibited finely granular and diffuse cytoplasmic fluorescence on immunostaining for FmoPV N protein. Electron microscopy showed enveloped virus with typical "herringbone" appearance of helical N in paramyxoviruses. Histological examination of necropsy tissues in two FmoPV-positive cats revealed interstitial inflammatory infiltrate and tubular degeneration/necrosis in kidneys, with decreased cauxin expression in degenerated tubular epithelial cells, compatible with tubulointerstitial nephritis (TIN). Immunohistochemical staining revealed FmoPV N protein-positive renal tubular cells and mononuclear cells in lymph nodes. A case-control study showed the presence of TIN in seven of 12 cats with FmoPV infection, but only two of 15 cats without FmoPV infection (P < 0.05), suggesting an association between FmoPV and TIN.
 
dc.description.natureLink_to_OA_fulltext
 
dc.identifier.citationProceedings of The National Academy of Sciences of The United States of America, 2012, v. 109 n. 14, p. 5435-5440 [How to Cite?]
DOI: http://dx.doi.org/10.1073/pnas.1119972109
 
dc.identifier.doihttp://dx.doi.org/10.1073/pnas.1119972109
 
dc.identifier.eissn1091-6490
 
dc.identifier.epage5440
 
dc.identifier.hkuros204318
 
dc.identifier.isiWOS:000302294700066
Funding AgencyGrant Number
Research Grants CouncilHKU 783611M
University of Hong Kong
Providence Foundation Limited
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health
Funding Information:

We thank Director Alan Chi-Kong Wong, Siu-Fai Leung, Thomas Hon-Chung Sit, and Howard Kai-Hay Wong [Agriculture Fisheries and Conservation Department (AFCD)]; Veterinary Officers of the AFCD Animal Management Centres for assistance and collection of samples; Dr. Chi-Kwan Yip, Dr. Cassius Chan, and Dr. King-Shun Lo for animal necropsy; Prof. Yamashita (Iwate University) for providing the anti-cat cauxin antibody; and Prof. K. C. Chan for the MAC387 antibody. We thank Mrs. Carol Yu, Prof. Richard Yu, Mr. Hui Hoy, and Mr. Hui Ming for support in genomic sequencing platform, and Ms. Eunice Lam for her donation for emerging infectious disease research. This work was supported in part by Research Grants Council Grant HKU 783611M; the Strategic Research Theme Fund and University Development Fund of the University of Hong Kong; the Providence Foundation Limited in memory of the late Dr. Lui Hac Minh; and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health.

 
dc.identifier.issn0027-8424
2012 Impact Factor: 9.737
2012 SCImago Journal Rankings: 5.473
 
dc.identifier.issue14
 
dc.identifier.pmcidPMC3325679
 
dc.identifier.pmid22431644
 
dc.identifier.scopuseid_2-s2.0-84859478260
 
dc.identifier.spage5435
 
dc.identifier.urihttp://hdl.handle.net/10722/157686
 
dc.identifier.volume109
 
dc.languageeng
 
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
 
dc.relation.projectMolecular evolution and interspecies jumping in picornaviruses
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAnimals, Domestic
 
dc.subject.meshBlotting, Western
 
dc.subject.meshCats
 
dc.subject.meshCell Line
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshMicroscopy, Electron
 
dc.subject.meshMorbillivirus - Pathogenicity
 
dc.subject.meshNephritis, Interstitial - Virology
 
dc.subject.meshPhylogeny
 
dc.subject.meshPolymerase Chain Reaction
 
dc.titleFeline morbillivirus, a previously undescribed paramyxovirus associated with tubulointerstitial nephritis in domestic cats
 
dc.typeArticle
 
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Author Affiliations
  1. Guangdong Center for Disease Control and Prevention
  2. The University of Hong Kong
  3. PathLab. Medical Laboratories