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Article: Antigen capture ELISA for specific detection of influenza A virus NS1 protein

TitleAntigen capture ELISA for specific detection of influenza A virus NS1 protein
Authors
KeywordsElisa
Influenza A Virus
Monoclonal Antibody
Nonstructural Protein
Issue Date2010
Citation
Chinese Journal Of Microbiology And Immunology, 2010, v. 30 n. 6, p. 566-569 How to Cite?
AbstractObjective: To develop a capture enzyme-linked immunosorbent assay (ELISA) for influenza A virus nonstructural protein NS1 using two anti-NS1 monoclonal antibodies (McAb) that map to distinct sites on the protein and to detect the NS1 in the culture supernatant and cell lysis supernatant of influenza virus. Methods: Among the McAb against influenza A virus NS1, antibody pairs were analyzed to choose the optimal coating McAb and detecting McAb by determinating the detection sensitivity and specificity. Results: The capture ELISA efficiently detected as little as 240 pg/ml of recombinant NS1 protein and exhibited no cross-reactivity for influenza B virus, parainfluenza virus NS1. Conclusion: A sensitive and specific NS1-based capture ELISA for influenza A virus NS1 was successfully established, which could be an important tool for diagnosis of influenza A virus infection.
Persistent Identifierhttp://hdl.handle.net/10722/157684
ISSN
2015 SCImago Journal Rankings: 0.108
References

 

DC FieldValueLanguage
dc.contributor.authorDing, XXen_US
dc.contributor.authorTsoi, HWen_US
dc.contributor.authorQiu, LWen_US
dc.contributor.authorWang, YDen_US
dc.contributor.authorYuan, GYen_US
dc.contributor.authorChe, XYen_US
dc.date.accessioned2012-08-08T08:52:14Z-
dc.date.available2012-08-08T08:52:14Z-
dc.date.issued2010en_US
dc.identifier.citationChinese Journal Of Microbiology And Immunology, 2010, v. 30 n. 6, p. 566-569en_US
dc.identifier.issn0254-5101en_US
dc.identifier.urihttp://hdl.handle.net/10722/157684-
dc.description.abstractObjective: To develop a capture enzyme-linked immunosorbent assay (ELISA) for influenza A virus nonstructural protein NS1 using two anti-NS1 monoclonal antibodies (McAb) that map to distinct sites on the protein and to detect the NS1 in the culture supernatant and cell lysis supernatant of influenza virus. Methods: Among the McAb against influenza A virus NS1, antibody pairs were analyzed to choose the optimal coating McAb and detecting McAb by determinating the detection sensitivity and specificity. Results: The capture ELISA efficiently detected as little as 240 pg/ml of recombinant NS1 protein and exhibited no cross-reactivity for influenza B virus, parainfluenza virus NS1. Conclusion: A sensitive and specific NS1-based capture ELISA for influenza A virus NS1 was successfully established, which could be an important tool for diagnosis of influenza A virus infection.en_US
dc.languageengen_US
dc.relation.ispartofChinese Journal of Microbiology and Immunologyen_US
dc.subjectElisaen_US
dc.subjectInfluenza A Virusen_US
dc.subjectMonoclonal Antibodyen_US
dc.subjectNonstructural Proteinen_US
dc.titleAntigen capture ELISA for specific detection of influenza A virus NS1 proteinen_US
dc.typeArticleen_US
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_US
dc.identifier.authorityTsoi, HW=rp00439en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3760/cma.j.issn.0254-5101.2010.06.018en_US
dc.identifier.scopuseid_2-s2.0-84863248156-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84858174253&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume30en_US
dc.identifier.issue6en_US
dc.identifier.spage566en_US
dc.identifier.epage569en_US
dc.identifier.scopusauthoridDing, XX=35765103800en_US
dc.identifier.scopusauthoridTsoi, HW=6603822102en_US
dc.identifier.scopusauthoridQiu, LW=55086589200en_US
dc.identifier.scopusauthoridWang, YD=55088532900en_US
dc.identifier.scopusauthoridYuan, GY=55091197100en_US
dc.identifier.scopusauthoridChe, XY=7005743182en_US

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