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Article: Detection of human rhinovirus C in fecal samples of children with gastroenteritis

TitleDetection of human rhinovirus C in fecal samples of children with gastroenteritis
Authors
KeywordsChildren
Fecal
Gastroenteritis
Human rhinovirus C
Pediatric
Stool
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2012, v. 53 n. 4, p. 290-296 How to Cite?
AbstractBackground: Despite recent discovery of the novel human rhinovirus species, HRV-C, little is known about the association of HRV-C in diseases other than respiratory tract infections. Objectives: To investigate the presence of HRV-C in fecal samples of children with gastroenteritis. Study design: 734 fecal samples from hospitalized children with gastroenteritis were subject to picornavirus detection by RT-PCR of the conserved 5'-NCR. Positive samples were subject to VP4 and 3D pol gene analysis for species determination. The clinical and molecular epidemiology of HRV-C and other picornaviruses was analyzed. Results: Picornaviruses were detected in 113 (15.4%) of 734 fecal samples from children with gastroenteritis by RT-PCR of 5'-NCR, with 58 containing potential HRVs and 55 containing other enteroviruses. PCR of the VP4 and 3D pol regions was positive in 21 and 19 samples respectively (both regions positive in 8 samples). Sequencing analysis showed the presence of HRV-C in four samples, and diverse picornaviruses including HRV-A (n=2), HEV-A (n=2), HEV-B (n=2), HEV-C (n=21) and HPeV (n=2) in other samples, with co-detection of HRV-C and HPeV in one sample. Of the four children with HRV-C detected in fecal samples, three presented with diarrhea in the absence of respiratory symptoms, while one also had acute bronchiolitis. The four HRV-C strains from fecal samples belonged to the existing clade of diverse HRV-C genotypes, indistinguishable from previous respiratory strains. Conclusions: HRV-C can be detected in fecal samples of children with gastroenteritis, in the absence of respiratory symptoms. This study also represented the first to detect HPeV in our population. © 2012 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/157683
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council, University Grant CouncilHKU 768709M
University of Hong Kong
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work is partly supported by the Research Grant Council Grant (HKU 768709M), University Grant Council; Strategic Research Theme Fund, and University Development Fund, The University of Hong Kong; and Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYip, CCYen_HK
dc.contributor.authorLung, DCen_HK
dc.contributor.authorLee, Pen_HK
dc.contributor.authorQue, TLen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2012-08-08T08:52:14Z-
dc.date.available2012-08-08T08:52:14Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Clinical Virology, 2012, v. 53 n. 4, p. 290-296en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/157683-
dc.description.abstractBackground: Despite recent discovery of the novel human rhinovirus species, HRV-C, little is known about the association of HRV-C in diseases other than respiratory tract infections. Objectives: To investigate the presence of HRV-C in fecal samples of children with gastroenteritis. Study design: 734 fecal samples from hospitalized children with gastroenteritis were subject to picornavirus detection by RT-PCR of the conserved 5'-NCR. Positive samples were subject to VP4 and 3D pol gene analysis for species determination. The clinical and molecular epidemiology of HRV-C and other picornaviruses was analyzed. Results: Picornaviruses were detected in 113 (15.4%) of 734 fecal samples from children with gastroenteritis by RT-PCR of 5'-NCR, with 58 containing potential HRVs and 55 containing other enteroviruses. PCR of the VP4 and 3D pol regions was positive in 21 and 19 samples respectively (both regions positive in 8 samples). Sequencing analysis showed the presence of HRV-C in four samples, and diverse picornaviruses including HRV-A (n=2), HEV-A (n=2), HEV-B (n=2), HEV-C (n=21) and HPeV (n=2) in other samples, with co-detection of HRV-C and HPeV in one sample. Of the four children with HRV-C detected in fecal samples, three presented with diarrhea in the absence of respiratory symptoms, while one also had acute bronchiolitis. The four HRV-C strains from fecal samples belonged to the existing clade of diverse HRV-C genotypes, indistinguishable from previous respiratory strains. Conclusions: HRV-C can be detected in fecal samples of children with gastroenteritis, in the absence of respiratory symptoms. This study also represented the first to detect HPeV in our population. © 2012 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.subjectChildrenen_HK
dc.subjectFecalen_HK
dc.subjectGastroenteritisen_HK
dc.subjectHuman rhinovirus Cen_HK
dc.subjectPediatricen_HK
dc.subjectStoolen_HK
dc.subject.meshFeces - virology-
dc.subject.meshGastroenteritis - epidemiology - virology-
dc.subject.meshPicornaviridae Infections - epidemiology - virology-
dc.subject.meshRNA, Viral - genetics-
dc.subject.meshRhinovirus - classification - genetics - isolation and purification-
dc.titleDetection of human rhinovirus C in fecal samples of children with gastroenteritisen_HK
dc.typeArticleen_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailYip, CCY: yipcyril@hku.hken_HK
dc.identifier.emailLau, YL: lauylung@hku.hken_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYip, CCY=rp01721en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jcv.2012.01.008en_HK
dc.identifier.pmid22317907-
dc.identifier.scopuseid_2-s2.0-84858152397en_HK
dc.identifier.hkuros204315-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84858152397&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue4en_HK
dc.identifier.spage290en_HK
dc.identifier.epage296en_HK
dc.identifier.isiWOS:000301358300004-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectThe role of a novel rhinovirus species, rhinovirus C, in respiratory illness and its molecular epidemiology-
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYip, CCY=14016999800en_HK
dc.identifier.scopusauthoridLung, DC=36902110000en_HK
dc.identifier.scopusauthoridLee, P=36189338700en_HK
dc.identifier.scopusauthoridQue, TL=7003786628en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike10326166-

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