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Article: Identification of a novel feline picornavirus from the domestic cat

TitleIdentification of a novel feline picornavirus from the domestic cat
Authors
Issue Date2012
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2012, v. 86 n. 1, p. 395-405 How to Cite?
AbstractWhile picornaviruses are known to infect different animals, their existence in the domestic cat was unknown. We describe the discovery of a novel feline picornavirus (FePV) from stray cats in Hong Kong. From samples from 662 cats, FePV was detected in fecal samples from 14 cats and urine samples from 2 cats by reverse transcription-PCR (RT-PCR). Analysis of five FePV genomes revealed a distinct phylogenetic position and genomic features, with low sequence homologies to known picornaviruses especially in leader and 2A proteins. Among the viruses that belong to the closely related bat picornavirus groups 1 to 3 and the genus Sapelovirus, G+C content and sequence analysis of P1, P2, and P3 regions showed that FePV is most closely related to bat picornavirus group 3. However, FePV possessed other distinct features, including a putative type IV internal ribosome entry site/segment (IRES) instead of type I IRES in bat picornavirus group 3, protein cleavage sites, and H-D-C catalytic triad in 3C pro different from those in sapeloviruses and bat picornaviruses, and the shortest leader protein among known picornaviruses. These results suggest that FePV may belong to a new genus in the family Picornaviridae. Western blot analysis using recombinant FePV VP1 polypeptide showed a high seroprevalence of 33.6% for IgG among the plasma samples from 232 cats tested. IgM was also detected in three cats positive for FePV in fecal samples, supporting recent infection in these cats. Further studies are important to understand the pathogenicity, epidemiology, and genetic evolution of FePV in these common pet animals. © 2012, American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/157673
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
HKSAR Department of Agriculture, Fisheries, and Conservation [AFCD]
University Grant CouncilHKU 783611 M
Committee for Research and Conference
Strategic Research Theme Fund
University Development Fund
University of Hong Kong
HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau
Providence Foundation Limited in memory of the late Lui Hac Minh
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

We thank Alan Chi-Kong Wong, Siu-Fai Leung, Thomas Hon-Chung Sit, and Howard Kai-Hay Wong (HKSAR Department of Agriculture, Fisheries, and Conservation [AFCD]) for facilitation and support and veterinary officers of the AFCD Animal Management Centres for assistance and collection of samples. We are grateful to the generous support of Carol Yu, Richard Yu, Hui Hoy, and Hui Ming in the genomic sequencing platform.

References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYip, CCYen_HK
dc.contributor.authorChoi, GKYen_HK
dc.contributor.authorWu, Yen_HK
dc.contributor.authorBai, Ren_HK
dc.contributor.authorFan, RYYen_HK
dc.contributor.authorLai, KKYen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2012-08-08T08:52:09Z-
dc.date.available2012-08-08T08:52:09Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Virology, 2012, v. 86 n. 1, p. 395-405en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/157673-
dc.description.abstractWhile picornaviruses are known to infect different animals, their existence in the domestic cat was unknown. We describe the discovery of a novel feline picornavirus (FePV) from stray cats in Hong Kong. From samples from 662 cats, FePV was detected in fecal samples from 14 cats and urine samples from 2 cats by reverse transcription-PCR (RT-PCR). Analysis of five FePV genomes revealed a distinct phylogenetic position and genomic features, with low sequence homologies to known picornaviruses especially in leader and 2A proteins. Among the viruses that belong to the closely related bat picornavirus groups 1 to 3 and the genus Sapelovirus, G+C content and sequence analysis of P1, P2, and P3 regions showed that FePV is most closely related to bat picornavirus group 3. However, FePV possessed other distinct features, including a putative type IV internal ribosome entry site/segment (IRES) instead of type I IRES in bat picornavirus group 3, protein cleavage sites, and H-D-C catalytic triad in 3C pro different from those in sapeloviruses and bat picornaviruses, and the shortest leader protein among known picornaviruses. These results suggest that FePV may belong to a new genus in the family Picornaviridae. Western blot analysis using recombinant FePV VP1 polypeptide showed a high seroprevalence of 33.6% for IgG among the plasma samples from 232 cats tested. IgM was also detected in three cats positive for FePV in fecal samples, supporting recent infection in these cats. Further studies are important to understand the pathogenicity, epidemiology, and genetic evolution of FePV in these common pet animals. © 2012, American Society for Microbiology.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.-
dc.rightsCopyright © American Society for Microbiology, [Journal of Virology, 2011, v. 86 n. 1, p. 395-405]-
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCat Diseases - virologyen_US
dc.subject.meshCatsen_US
dc.subject.meshPicornaviridae - chemistry - classification - genetics - isolation and purificationen_US
dc.subject.meshPicornaviridae Infections - veterinary - virologyen_US
dc.titleIdentification of a novel feline picornavirus from the domestic caten_HK
dc.typeArticleen_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailYip, CCY: yipcyril@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYip, CCY=rp01721en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/JVI.06253-11en_HK
dc.identifier.pmid22031936-
dc.identifier.pmcidPMC3255865-
dc.identifier.scopuseid_2-s2.0-84862908825en_HK
dc.identifier.hkuros204210-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862908825&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume86en_HK
dc.identifier.issue1en_HK
dc.identifier.spage395en_HK
dc.identifier.epage405en_HK
dc.identifier.eissn1098-5514-
dc.identifier.isiWOS:000298347700038-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, PCY=55177222700en_HK
dc.identifier.scopusauthoridYip, CCY=14016999800en_HK
dc.identifier.scopusauthoridChoi, GKY=35423450200en_HK
dc.identifier.scopusauthoridWu, Y=54898602600en_HK
dc.identifier.scopusauthoridBai, R=54896689400en_HK
dc.identifier.scopusauthoridFan, RYY=15519269300en_HK
dc.identifier.scopusauthoridLai, KKY=36708103200en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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