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Article: First report of disseminated Mycobacterium skin infections in two liver transplant recipients and rapid diagnosis by hsp65 gene sequencing

TitleFirst report of disseminated Mycobacterium skin infections in two liver transplant recipients and rapid diagnosis by hsp65 gene sequencing
Authors
Issue Date2011
PublisherAmerican Society for Microbiology.
Citation
Journal of Clinical Microbiology, 2011, v. 49 n. 11, p. 3733-3737 How to Cite?
AbstractWe present here the first report of disseminated skin Mycobacterium infections in two liver transplant recipients, in which hsp65 gene sequencing was used for rapid species identification. Both patients had hepatitis B virus-related cirrhosis and diabetes mellitus and presented with progressive generalized, nodular skin lesions. In one patient, a 50-year-old woman who had frequent contact with marine fish, an acid-fast bacillus was isolated from skin biopsy tissue after 2 months of culture. While awaiting phenotypic identification results, hsp65 gene sequencing showed that it was most closely related to that of Mycobacterium marinum with 100% nucleotide identity. The patient was treated with oral rifampin, ethambutol, and moxifloxacin. In the other patient, a 59-year-old woman, direct PCR for Mycobacterium using hsp65 gene from skin biopsy tissue was positive, with the sequence most closely related to that of M. haemophilum with 100% nucleotide identity. Based on PCR results, the patient was treated with clarithromycin, ethambutol, moxifloxacin, and amikacin. A strain of M. haemophilum was only isolated after 3 months. Skin lesions of both patients resolved after 1 year of antimycobacterial therapy. Nontuberculous Mycobacterium infections should be considered in liver transplant recipients presenting with chronic, nodular skin lesions. This report highlights the crucial role of hsp65 gene PCR and sequencing on both cultured isolates and direct clinical specimens for rapid diagnosis of slow-growing Mycobacterium infection.
Persistent Identifierhttp://hdl.handle.net/10722/157653
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Committee for Research and Conference
University of Hong Kong
Health, Welfare, and Food Bureau
Funding Information:

This study was partly supported by the Committee for Research and Conference Grant, The University of Hong Kong, and by the HKSAR Research Fund for the Control of Infectious Diseases (Commissioned Study) of the Health, Welfare, and Food Bureau.

References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_US
dc.contributor.authorCurreem, SOTen_US
dc.contributor.authorNgan, AHYen_US
dc.contributor.authorYeung, CKen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorWoo, PCYen_US
dc.date.accessioned2012-08-08T08:51:58Z-
dc.date.available2012-08-08T08:51:58Z-
dc.date.issued2011en_US
dc.identifier.citationJournal of Clinical Microbiology, 2011, v. 49 n. 11, p. 3733-3737en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/157653-
dc.description.abstractWe present here the first report of disseminated skin Mycobacterium infections in two liver transplant recipients, in which hsp65 gene sequencing was used for rapid species identification. Both patients had hepatitis B virus-related cirrhosis and diabetes mellitus and presented with progressive generalized, nodular skin lesions. In one patient, a 50-year-old woman who had frequent contact with marine fish, an acid-fast bacillus was isolated from skin biopsy tissue after 2 months of culture. While awaiting phenotypic identification results, hsp65 gene sequencing showed that it was most closely related to that of Mycobacterium marinum with 100% nucleotide identity. The patient was treated with oral rifampin, ethambutol, and moxifloxacin. In the other patient, a 59-year-old woman, direct PCR for Mycobacterium using hsp65 gene from skin biopsy tissue was positive, with the sequence most closely related to that of M. haemophilum with 100% nucleotide identity. Based on PCR results, the patient was treated with clarithromycin, ethambutol, moxifloxacin, and amikacin. A strain of M. haemophilum was only isolated after 3 months. Skin lesions of both patients resolved after 1 year of antimycobacterial therapy. Nontuberculous Mycobacterium infections should be considered in liver transplant recipients presenting with chronic, nodular skin lesions. This report highlights the crucial role of hsp65 gene PCR and sequencing on both cultured isolates and direct clinical specimens for rapid diagnosis of slow-growing Mycobacterium infection.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology.-
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.-
dc.rightsCopyright © American Society for Microbiology, [Journal of Clinical Microbiology, 2011, v. 49 n. 11, p. 3733-3737]-
dc.subject.meshLiver Transplantationen_US
dc.subject.meshMycobacterium Infections - diagnosis - drug therapy - microbiology - pathologyen_US
dc.subject.meshMycobacterium haemophilum - genetics - isolation and purificationen_US
dc.subject.meshMycobacterium marinum - genetics - isolation and purificationen_US
dc.subject.meshSkin Diseases, Bacterial - diagnosis - microbiology - pathologyen_US
dc.titleFirst report of disseminated Mycobacterium skin infections in two liver transplant recipients and rapid diagnosis by hsp65 gene sequencingen_US
dc.typeArticleen_US
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_US
dc.identifier.emailCurreem, SOT: shirly@hkucc.hku.hken_US
dc.identifier.emailYeung, CK: drcyeung@hku.hken_US
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/JCM.05088-11en_US
dc.identifier.pmid21880973-
dc.identifier.pmcidPMC3209108-
dc.identifier.scopuseid_2-s2.0-80355126268en_US
dc.identifier.hkuros204118-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80355126268&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume49en_US
dc.identifier.issue11en_US
dc.identifier.spage3733en_US
dc.identifier.epage3737en_US
dc.identifier.isiWOS:000296617300003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridYeung, CK=7201354123en_US
dc.identifier.scopusauthoridNgan, AHY=14037517900en_US
dc.identifier.scopusauthoridCurreem, SOT=16416762100en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US

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