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Article: Clinical and virological factors associated with viremia in pandemic influenza A/H1N1/2009 virus infection

TitleClinical and virological factors associated with viremia in pandemic influenza A/H1N1/2009 virus infection
Authors
Issue Date2011
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2011, v. 6 n. 9, article no. e22534 How to Cite?
AbstractBACKGROUND: Positive detection of viral RNA in blood and other non-respiratory specimens occurs in severe human influenza A/H5N1 viral infection but is not known to occur commonly in seasonal human influenza infection. Recently, viral RNA was detected in the blood of patients suffering from severe pandemic influenza A/H1N1/2009 viral infection, although the significance of viremia had not been previously studied. Our study aims to explore the clinical and virological factors associated with pandemic influenza A/H1N1/2009 viremia and to determine its clinical significance. METHODOLOGY/PRINCIPAL FINDINGS: Clinical data of patients admitted to hospitals in Hong Kong between May 2009 and April 2010 and tested positive for pandemic influenza A/H1N1/2009 was collected. Viral RNA was detected by reverse-transcription polymerase chain reactions (RT-PCR) targeting the matrix (M) and HA genes of pandemic influenza A/H1N1/2009 virus from the following specimens: nasopharyngeal aspirate (NPA), endotracheal aspirate (ETA), blood, stool and rectal swab. Stool and/ or rectal swab was obtained only if the patient complained of any gastrointestinal symptoms. A total of 139 patients were included in the study, with viral RNA being detected in the blood of 14 patients by RT-PCR. The occurrence of viremia was strongly associated with a severe clinical presentation and a higher mortality rate, although the latter association was not statistically significant. D222G/N quasispecies were observed in 90% of the blood samples. CONCLUSION: Presence of pandemic influenza A/H1N1/2009 viremia is an indicator of disease severity and strongly associated with D222G/N mutation in the viral hemagglutinin protein.
Persistent Identifierhttp://hdl.handle.net/10722/157646
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Providence Foundation Limited
University Grants Council
Food and Health Bureau of the Hong Kong SAR Government
Funding Information:

We gratefully acknowledge funding support from the Providence Foundation Limited in memory of the late Dr Lui Hac Minh, the University Grants Council, and the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Hong Kong SAR Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorTse, Hen_US
dc.contributor.authorTo, KKWen_US
dc.contributor.authorWen, Xen_US
dc.contributor.authorChen, Hen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorTsoi, HWen_US
dc.contributor.authorLi, IWSen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:51:55Z-
dc.date.available2012-08-08T08:51:55Z-
dc.date.issued2011en_US
dc.identifier.citationPLoS One, 2011, v. 6 n. 9, article no. e22534en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10722/157646-
dc.description.abstractBACKGROUND: Positive detection of viral RNA in blood and other non-respiratory specimens occurs in severe human influenza A/H5N1 viral infection but is not known to occur commonly in seasonal human influenza infection. Recently, viral RNA was detected in the blood of patients suffering from severe pandemic influenza A/H1N1/2009 viral infection, although the significance of viremia had not been previously studied. Our study aims to explore the clinical and virological factors associated with pandemic influenza A/H1N1/2009 viremia and to determine its clinical significance. METHODOLOGY/PRINCIPAL FINDINGS: Clinical data of patients admitted to hospitals in Hong Kong between May 2009 and April 2010 and tested positive for pandemic influenza A/H1N1/2009 was collected. Viral RNA was detected by reverse-transcription polymerase chain reactions (RT-PCR) targeting the matrix (M) and HA genes of pandemic influenza A/H1N1/2009 virus from the following specimens: nasopharyngeal aspirate (NPA), endotracheal aspirate (ETA), blood, stool and rectal swab. Stool and/ or rectal swab was obtained only if the patient complained of any gastrointestinal symptoms. A total of 139 patients were included in the study, with viral RNA being detected in the blood of 14 patients by RT-PCR. The occurrence of viremia was strongly associated with a severe clinical presentation and a higher mortality rate, although the latter association was not statistically significant. D222G/N quasispecies were observed in 90% of the blood samples. CONCLUSION: Presence of pandemic influenza A/H1N1/2009 viremia is an indicator of disease severity and strongly associated with D222G/N mutation in the viral hemagglutinin protein.en_US
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_US
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshHemagglutinins, Viral - metabolism-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - metabolism-
dc.subject.meshInfluenza, Human - virology-
dc.subject.meshPolymorphism, Genetic-
dc.subject.meshViremia - diagnosis - epidemiology-
dc.titleClinical and virological factors associated with viremia in pandemic influenza A/H1N1/2009 virus infectionen_US
dc.typeArticleen_US
dc.identifier.emailTse, H: herman@graduate.hku.hken_US
dc.identifier.emailTo, KKW: kelvinto@hkucc.hku.hken_US
dc.identifier.emailChen, H: hlchen@hku.hken_US
dc.identifier.emailTsoi, HW: hwtsoi@hkucc.hku.hken_US
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_US
dc.identifier.authorityTse, H=rp00519en_US
dc.identifier.authorityTo, KKW=rp01384en_US
dc.identifier.authorityChen, H=rp00383en_US
dc.identifier.authorityTsoi, HW=rp00439en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1371/journal.pone.0022534en_US
dc.identifier.pmid21980333-
dc.identifier.pmcidPMC3181248-
dc.identifier.scopuseid_2-s2.0-80053206038en_US
dc.identifier.hkuros208518-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053206038&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6en_US
dc.identifier.issue9, article no. e22534en_US
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000295933700001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridLi, IWS=24464179500en_US
dc.identifier.scopusauthoridTsoi, HW=6603822102en_US
dc.identifier.scopusauthoridChan, KH=7406034307en_US
dc.identifier.scopusauthoridChen, H=26643315400en_US
dc.identifier.scopusauthoridWen, X=36060825600en_US
dc.identifier.scopusauthoridTo, KKW=14323807300en_US
dc.identifier.scopusauthoridTse, H=7006070596en_US

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