File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Extensive dissemination of CTX-M-producing Escherichia coli with multidrug resistance to 'critically important' antibiotics among food animals in Hong Kong, 2008-10

TitleExtensive dissemination of CTX-M-producing Escherichia coli with multidrug resistance to 'critically important' antibiotics among food animals in Hong Kong, 2008-10
Authors
KeywordsColonization
Extended-spectrum β-lactamases
Molecular epidemiology
Issue Date2011
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 765-768 How to Cite?
AbstractObjectives: To assess the occurrence of faecal carriage of Escherichia coli with resistance to 'critically important' antibiotics in various animals. Methods: Rectal or cloacal swabs were obtained weekly from cattle, pigs, chickens, cats, dogs and wild rodents over a 2 year period. Plain and antibiotic-containing medium was used for bacterial isolation. Selected isolates were characterized by molecular methods. Results: In total, 2106 faecal specimens from 398 cats, 460 chickens, 368 dogs, 210 cattle, 214 pigs and 456 rodents were cultured. The faecal carriage rate of extended-spectrum β-lactamase (ESBL)-producing E. coli was highest in pigs (63.6%, 136/214) and lowest in rodents (4.2%, 19/456). The faecal ESBL-producing E. coli carriage rate for food-producing animals (53.6%, 474/884) was significantly higher than that for cats/dogs (14.0%, 107/766; P,0.01) and wild rodents (4.2%, 19/456; P,0.01). ESBL-producing isolates from food animals often (33%-81%) had multidrug (≥4) resistance to amikacin, chloramphenicol, ciprofloxacin, co-trimoxazole, gentamicin, nalidixic acid, netilmicin, nitrofurantoin and tetracycline. Most (91.2%) of the ESBL-producing isolates had CTX-M-type enzymes. A total of 10 alleles (3, 13, 14, 15, 24, 27, 28, 55, 65 and 98) from two CTX-M families (M1 and M9) were found. PFGE showed that the CTX-M-producing isolates were genetically diverse. Conclusions: This study shows that food animals are a major reservoir of E. coli with multidrug resistance to many antibiotics that are ranked as critically important in human medicine. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157624
ISSN
2015 Impact Factor: 4.919
2015 SCImago Journal Rankings: 2.157
ISI Accession Number ID
Funding AgencyGrant Number
Health, Welfare and Food Bureau of the Government of the HKSAR
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Government of the HKSAR, and the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_HK
dc.contributor.authorChow, KHen_HK
dc.contributor.authorLai, ELen_HK
dc.contributor.authorLo, WUen_HK
dc.contributor.authorYeung, MKen_HK
dc.contributor.authorChan, Jen_HK
dc.contributor.authorChan, PYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2012-08-08T08:51:46Z-
dc.date.available2012-08-08T08:51:46Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 765-768en_HK
dc.identifier.issn0305-7453en_HK
dc.identifier.urihttp://hdl.handle.net/10722/157624-
dc.description.abstractObjectives: To assess the occurrence of faecal carriage of Escherichia coli with resistance to 'critically important' antibiotics in various animals. Methods: Rectal or cloacal swabs were obtained weekly from cattle, pigs, chickens, cats, dogs and wild rodents over a 2 year period. Plain and antibiotic-containing medium was used for bacterial isolation. Selected isolates were characterized by molecular methods. Results: In total, 2106 faecal specimens from 398 cats, 460 chickens, 368 dogs, 210 cattle, 214 pigs and 456 rodents were cultured. The faecal carriage rate of extended-spectrum β-lactamase (ESBL)-producing E. coli was highest in pigs (63.6%, 136/214) and lowest in rodents (4.2%, 19/456). The faecal ESBL-producing E. coli carriage rate for food-producing animals (53.6%, 474/884) was significantly higher than that for cats/dogs (14.0%, 107/766; P,0.01) and wild rodents (4.2%, 19/456; P,0.01). ESBL-producing isolates from food animals often (33%-81%) had multidrug (≥4) resistance to amikacin, chloramphenicol, ciprofloxacin, co-trimoxazole, gentamicin, nalidixic acid, netilmicin, nitrofurantoin and tetracycline. Most (91.2%) of the ESBL-producing isolates had CTX-M-type enzymes. A total of 10 alleles (3, 13, 14, 15, 24, 27, 28, 55, 65 and 98) from two CTX-M families (M1 and M9) were found. PFGE showed that the CTX-M-producing isolates were genetically diverse. Conclusions: This study shows that food animals are a major reservoir of E. coli with multidrug resistance to many antibiotics that are ranked as critically important in human medicine. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_HK
dc.subjectColonizationen_HK
dc.subjectExtended-spectrum β-lactamasesen_HK
dc.subjectMolecular epidemiologyen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Domestic - Microbiologyen_US
dc.subject.meshAnti-Bacterial Agents - Pharmacologyen_US
dc.subject.meshCarrier State - Microbiologyen_US
dc.subject.meshCloaca - Microbiologyen_US
dc.subject.meshDrug Resistance, Multiple, Bacterialen_US
dc.subject.meshEscherichia Coli - Drug Effects - Enzymology - Isolation & Purificationen_US
dc.subject.meshEscherichia Coli Infections - Microbiologyen_US
dc.subject.meshHong Kongen_US
dc.subject.meshRectum - Microbiologyen_US
dc.subject.meshRodentia - Microbiologyen_US
dc.subject.meshBeta-Lactamases - Biosynthesisen_US
dc.titleExtensive dissemination of CTX-M-producing Escherichia coli with multidrug resistance to 'critically important' antibiotics among food animals in Hong Kong, 2008-10en_HK
dc.typeArticleen_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailChow, KH: khchowb@hku.hken_HK
dc.identifier.emailChan, J: jfwchan@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityChan, J=rp01736en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1093/jac/dkq539en_HK
dc.identifier.pmid21393133-
dc.identifier.scopuseid_2-s2.0-79952783031en_HK
dc.identifier.hkuros209814-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952783031&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue4en_HK
dc.identifier.spage765en_HK
dc.identifier.epage768en_HK
dc.identifier.eissn1460-2091-
dc.identifier.isiWOS:000288551300013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridLai, EL=8238477100en_HK
dc.identifier.scopusauthoridLo, WU=35558916700en_HK
dc.identifier.scopusauthoridYeung, MK=37040000300en_HK
dc.identifier.scopusauthoridChan, J=24278817900en_HK
dc.identifier.scopusauthoridChan, PY=36509642500en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike9087357-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats