Article: Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong

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TitleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
AuthorsHo, PL2
Lo, WU2
Wong, RCW1
Yeung, MK2
Chow, KH2
Que, TL1
Tong, AHY2
Bao, JYJ2
Lok, S2
Wong, SSY2
KeywordsAntimicrobial drug resistance
CTX-M-14 β-lactamase
Enterobacteriaceae
Plasmids
Restriction fragment length
Issue Date2011
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
CitationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dkr010
AbstractObjectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
ISSN0305-7453
2011 Impact Factor: 5.068
2011 SCImago Journal Rankings: 0.422
DOIhttp://dx.doi.org/10.1093/jac/dkr010
ISI Accession Number IDWOS:000288551300011
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHo, PL
dc.contributor.authorLo, WU
dc.contributor.authorWong, RCW
dc.contributor.authorYeung, MK
dc.contributor.authorChow, KH
dc.contributor.authorQue, TL
dc.contributor.authorTong, AHY
dc.contributor.authorBao, JYJ
dc.contributor.authorLok, S
dc.contributor.authorWong, SSY
dc.date.accessioned2012-08-08T08:51:45Z
dc.date.available2012-08-08T08:51:45Z
dc.date.issued2011
dc.description.abstractObjectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dkr010
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dkr010
dc.identifier.epage756
dc.identifier.hkuros209817
dc.identifier.isiWOS:000288551300011
dc.identifier.issn0305-7453
2011 Impact Factor: 5.068
2011 SCImago Journal Rankings: 0.422
dc.identifier.issue4
dc.identifier.pmid21393220
dc.identifier.scopuseid_2-s2.0-79952779097
dc.identifier.spage752
dc.identifier.urihttp://hdl.handle.net/10722/157623
dc.identifier.volume66
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
dc.relation.referencesReferences in Scopus
dc.subject.meshAdult
dc.subject.meshChild
dc.subject.meshChild, Preschool
dc.subject.meshConjugation, Genetic
dc.subject.meshCystitis - Microbiology
dc.subject.meshDna, Bacterial - Chemistry - Genetics
dc.subject.meshEscherichia Coli - Enzymology - Genetics - Isolation & Purification
dc.subject.meshEscherichia Coli Infections - Microbiology
dc.subject.meshFemale
dc.subject.meshGenotype
dc.subject.meshHong Kong
dc.subject.meshHumans
dc.subject.meshMolecular Sequence Data
dc.subject.meshPlasmids
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshPolymorphism, Restriction Fragment Length
dc.subject.meshSequence Analysis, Dna
dc.subject.meshBeta-Lactamases - Genetics
dc.subjectAntimicrobial drug resistance
dc.subjectCTX-M-14 β-lactamase
dc.subjectEnterobacteriaceae
dc.subjectPlasmids
dc.subjectRestriction fragment length
dc.titleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
dc.typeArticle
Author Affiliations
  1. Tuen Mun Hospital
  2. The University of Hong Kong