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Article: Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong

TitleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
Authors
KeywordsAntimicrobial drug resistance
CTX-M-14 β-lactamase
Enterobacteriaceae
Plasmids
Restriction fragment length
Issue Date2011
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756 How to Cite?
Abstract
Objectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157623
ISSN
2013 Impact Factor: 5.439
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. Tuen Mun Hospital
DC FieldValueLanguage
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLo, WUen_HK
dc.contributor.authorWong, RCWen_HK
dc.contributor.authorYeung, MKen_HK
dc.contributor.authorChow, KHen_HK
dc.contributor.authorQue, TLen_HK
dc.contributor.authorTong, AHYen_HK
dc.contributor.authorBao, JYJen_HK
dc.contributor.authorLok, Sen_HK
dc.contributor.authorWong, SSYen_HK
dc.date.accessioned2012-08-08T08:51:45Z-
dc.date.available2012-08-08T08:51:45Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756en_HK
dc.identifier.issn0305-7453en_HK
dc.identifier.urihttp://hdl.handle.net/10722/157623-
dc.description.abstractObjectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_HK
dc.subjectAntimicrobial drug resistanceen_HK
dc.subjectCTX-M-14 β-lactamaseen_HK
dc.subjectEnterobacteriaceaeen_HK
dc.subjectPlasmidsen_HK
dc.subjectRestriction fragment lengthen_HK
dc.subject.meshAdulten_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshConjugation, Geneticen_US
dc.subject.meshCystitis - Microbiologyen_US
dc.subject.meshDna, Bacterial - Chemistry - Geneticsen_US
dc.subject.meshEscherichia Coli - Enzymology - Genetics - Isolation & Purificationen_US
dc.subject.meshEscherichia Coli Infections - Microbiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshBeta-Lactamases - Geneticsen_US
dc.titleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailChow, KH: khchowb@hku.hken_HK
dc.identifier.emailLok, S: silok@genome.hku.hken_HK
dc.identifier.emailWong, SSY: samsonsy@hkucc.hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1093/jac/dkr010en_HK
dc.identifier.pmid21393220en_HK
dc.identifier.scopuseid_2-s2.0-79952779097en_HK
dc.identifier.hkuros209817-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952779097&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue4en_HK
dc.identifier.spage752en_HK
dc.identifier.epage756en_HK
dc.identifier.eissn1460-2091-
dc.identifier.isiWOS:000288551300011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLo, WU=35558916700en_HK
dc.identifier.scopusauthoridWong, RCW=8612000100en_HK
dc.identifier.scopusauthoridYeung, MK=37040000300en_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridQue, TL=7003786628en_HK
dc.identifier.scopusauthoridTong, AHY=7103351716en_HK
dc.identifier.scopusauthoridBao, JYJ=45960893700en_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK

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