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Article: Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
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TitleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
 
AuthorsHo, PL1
Lo, WU1
Wong, RCW2
Yeung, MK1
Chow, KH1
Que, TL2
Tong, AHY1
Bao, JYJ1
Lok, S1
Wong, SSY1
 
KeywordsAntimicrobial drug resistance
CTX-M-14 β-lactamase
Enterobacteriaceae
Plasmids
Restriction fragment length
 
Issue Date2011
 
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
CitationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dkr010
 
AbstractObjectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
 
ISSN0305-7453
2012 Impact Factor: 5.338
2012 SCImago Journal Rankings: 1.814
 
DOIhttp://dx.doi.org/10.1093/jac/dkr010
 
ISI Accession Number IDWOS:000288551300011
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHo, PL
 
dc.contributor.authorLo, WU
 
dc.contributor.authorWong, RCW
 
dc.contributor.authorYeung, MK
 
dc.contributor.authorChow, KH
 
dc.contributor.authorQue, TL
 
dc.contributor.authorTong, AHY
 
dc.contributor.authorBao, JYJ
 
dc.contributor.authorLok, S
 
dc.contributor.authorWong, SSY
 
dc.date.accessioned2012-08-08T08:51:45Z
 
dc.date.available2012-08-08T08:51:45Z
 
dc.date.issued2011
 
dc.description.abstractObjectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dkr010
 
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dkr010
 
dc.identifier.eissn1460-2091
 
dc.identifier.epage756
 
dc.identifier.hkuros209817
 
dc.identifier.isiWOS:000288551300011
 
dc.identifier.issn0305-7453
2012 Impact Factor: 5.338
2012 SCImago Journal Rankings: 1.814
 
dc.identifier.issue4
 
dc.identifier.pmid21393220
 
dc.identifier.scopuseid_2-s2.0-79952779097
 
dc.identifier.spage752
 
dc.identifier.urihttp://hdl.handle.net/10722/157623
 
dc.identifier.volume66
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdult
 
dc.subject.meshChild
 
dc.subject.meshChild, Preschool
 
dc.subject.meshConjugation, Genetic
 
dc.subject.meshCystitis - Microbiology
 
dc.subject.meshDna, Bacterial - Chemistry - Genetics
 
dc.subject.meshEscherichia Coli - Enzymology - Genetics - Isolation & Purification
 
dc.subject.meshEscherichia Coli Infections - Microbiology
 
dc.subject.meshFemale
 
dc.subject.meshGenotype
 
dc.subject.meshHong Kong
 
dc.subject.meshHumans
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshPlasmids
 
dc.subject.meshPolymerase Chain Reaction
 
dc.subject.meshPolymorphism, Restriction Fragment Length
 
dc.subject.meshSequence Analysis, Dna
 
dc.subject.meshBeta-Lactamases - Genetics
 
dc.subjectAntimicrobial drug resistance
 
dc.subjectCTX-M-14 β-lactamase
 
dc.subjectEnterobacteriaceae
 
dc.subjectPlasmids
 
dc.subjectRestriction fragment length
 
dc.titleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
 
dc.typeArticle
 
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<description.abstract>Objectives: We characterized plasmids encoding CTX-M-14 &#946;-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-I&#947; (n=4), F1B (n=2), FII and I1-I&#947; (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. &#169; The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Tuen Mun Hospital