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Article: Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
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TitleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
 
AuthorsHo, PL1 1
Lo, WU1
Wong, RCW2
Yeung, MK1
Chow, KH1
Que, TL2
Tong, AHY1
Bao, JYJ1
Lok, S1
Wong, SSY1 1
 
KeywordsAntimicrobial drug resistance
CTX-M-14 β-lactamase
Enterobacteriaceae
Plasmids
Restriction fragment length
 
Issue Date2011
 
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
CitationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dkr010
 
AbstractObjectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
 
ISSN0305-7453
2012 Impact Factor: 5.338
2012 SCImago Journal Rankings: 1.814
 
DOIhttp://dx.doi.org/10.1093/jac/dkr010
 
ISI Accession Number IDWOS:000288551300011
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHo, PL
 
dc.contributor.authorLo, WU
 
dc.contributor.authorWong, RCW
 
dc.contributor.authorYeung, MK
 
dc.contributor.authorChow, KH
 
dc.contributor.authorQue, TL
 
dc.contributor.authorTong, AHY
 
dc.contributor.authorBao, JYJ
 
dc.contributor.authorLok, S
 
dc.contributor.authorWong, SSY
 
dc.date.accessioned2012-08-08T08:51:45Z
 
dc.date.available2012-08-08T08:51:45Z
 
dc.date.issued2011
 
dc.description.abstractObjectives: We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2011, v. 66 n. 4, p. 752-756 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dkr010
 
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dkr010
 
dc.identifier.eissn1460-2091
 
dc.identifier.epage756
 
dc.identifier.hkuros209817
 
dc.identifier.isiWOS:000288551300011
 
dc.identifier.issn0305-7453
2012 Impact Factor: 5.338
2012 SCImago Journal Rankings: 1.814
 
dc.identifier.issue4
 
dc.identifier.pmid21393220
 
dc.identifier.scopuseid_2-s2.0-79952779097
 
dc.identifier.spage752
 
dc.identifier.urihttp://hdl.handle.net/10722/157623
 
dc.identifier.volume66
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdult
 
dc.subject.meshChild
 
dc.subject.meshChild, Preschool
 
dc.subject.meshConjugation, Genetic
 
dc.subject.meshCystitis - Microbiology
 
dc.subject.meshDna, Bacterial - Chemistry - Genetics
 
dc.subject.meshEscherichia Coli - Enzymology - Genetics - Isolation & Purification
 
dc.subject.meshEscherichia Coli Infections - Microbiology
 
dc.subject.meshFemale
 
dc.subject.meshGenotype
 
dc.subject.meshHong Kong
 
dc.subject.meshHumans
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshPlasmids
 
dc.subject.meshPolymerase Chain Reaction
 
dc.subject.meshPolymorphism, Restriction Fragment Length
 
dc.subject.meshSequence Analysis, Dna
 
dc.subject.meshBeta-Lactamases - Genetics
 
dc.subjectAntimicrobial drug resistance
 
dc.subjectCTX-M-14 β-lactamase
 
dc.subjectEnterobacteriaceae
 
dc.subjectPlasmids
 
dc.subjectRestriction fragment length
 
dc.titleComplete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong
 
dc.typeArticle
 
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<contributor.author>Chow, KH</contributor.author>
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<description.abstract>Objectives: We characterized plasmids encoding CTX-M-14 &#946;-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004. Methods: Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data. Results: The bla CTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-I&#947; (n=4), F1B (n=2), FII and I1-I&#947; (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing bla CTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to bla CTX-M-14 (pKF3-70) and bla CTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles. Conclusions: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community. &#169; The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Tuen Mun Hospital