File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: High diversity of polyketide synthase genes and the melanin biosynthesis gene cluster in Penicillium marneffei

TitleHigh diversity of polyketide synthase genes and the melanin biosynthesis gene cluster in Penicillium marneffei
Authors
Issue Date2010
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.febsjournal.org/
Citation
Febs Journal, 2010, v. 277 n. 18, p. 3750-3758 How to Cite?
AbstractDespite the unique phenotypic properties and clinical importance of Penicillium marneffei, the polyketide synthase genes in its genome have never been characterized. Twenty-three putative polyketide synthase genes and two putative polyketide synthase nonribosomal peptide-synthase hybrid genes were identified in the P. marneffei genome, a diversity much higher than found in other pathogenic thermal dimorphic fungi, such as Histoplasma capsulatum (one polyketide synthase gene) and Coccidioides immitis (10 polyketide synthase genes). These genes were evenly distributed on the phylogenetic tree with polyketide synthase genes of Aspergillus and other fungi, indicating that the high diversity was not a result of lineage-specific gene expansion through recent gene duplication. The melanin-biosynthesis gene cluster had gene order and orientations identical to those in the Talaromyces stipitatus (a teleomorph of Penicillium emmonsii) genome. Phylogenetically, all six genes of the melanin-biosynthesis gene cluster in P. marneffei were also most closely related to those in T. stipitatus, with high bootstrap supports. The polyketide synthase gene of the melanin-biosynthesis gene cluster (alb1) in P. marneffei was knocked down, which was accompanied by loss of melanin pigment production and reduced ornamentation in conidia. The survival of mice challenged with the alb1 knockdown mutant was significantly better than those challenged with wild-type P. marneffei (P < 0.005). The sterilizing doses of hydrogen peroxide, leading to a 50% reduction in survival of conidia, were 11 min for wild-type P. marneffei and 6 min for the alb1 knockdown mutant of P. marneffei, implying that the melanin-biosynthesis gene cluster contributed to virulence through decreased susceptibility to killing by hydrogen peroxide. © 2010 FEBS.
Persistent Identifierhttp://hdl.handle.net/10722/157600
ISSN
2015 Impact Factor: 4.237
2015 SCImago Journal Rankings: 2.141
ISI Accession Number ID
Funding AgencyGrant Number
Health, Welfare and Food Bureau of the Hong Kong SAR Government
Funding Information:

This work was partly supported by the Research Fund for the Control of Infectious Diseases (Commissioned study) of the Health, Welfare and Food Bureau of the Hong Kong SAR Government, Research Grant Council Grant, University Development Fund, Committee for Conference and Research Grant, Providence Foundation Limited in memory of the late Dr Lui Hac Minh, HKU Award for CAE Membership, HKU Medical Faculty Award for CAE Membership, Dr Hector T. G. Ma. We thank the Fungal Genetics Stock Center for providing the pSilent-1 vector.

References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorTam, EWTen_US
dc.contributor.authorChong, KTKen_US
dc.contributor.authorCai, JJen_US
dc.contributor.authorTung, ETKen_US
dc.contributor.authorNgan, AHYen_US
dc.contributor.authorLau, SKPen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:51:35Z-
dc.date.available2012-08-08T08:51:35Z-
dc.date.issued2010en_US
dc.identifier.citationFebs Journal, 2010, v. 277 n. 18, p. 3750-3758en_US
dc.identifier.issn1742-464Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/157600-
dc.description.abstractDespite the unique phenotypic properties and clinical importance of Penicillium marneffei, the polyketide synthase genes in its genome have never been characterized. Twenty-three putative polyketide synthase genes and two putative polyketide synthase nonribosomal peptide-synthase hybrid genes were identified in the P. marneffei genome, a diversity much higher than found in other pathogenic thermal dimorphic fungi, such as Histoplasma capsulatum (one polyketide synthase gene) and Coccidioides immitis (10 polyketide synthase genes). These genes were evenly distributed on the phylogenetic tree with polyketide synthase genes of Aspergillus and other fungi, indicating that the high diversity was not a result of lineage-specific gene expansion through recent gene duplication. The melanin-biosynthesis gene cluster had gene order and orientations identical to those in the Talaromyces stipitatus (a teleomorph of Penicillium emmonsii) genome. Phylogenetically, all six genes of the melanin-biosynthesis gene cluster in P. marneffei were also most closely related to those in T. stipitatus, with high bootstrap supports. The polyketide synthase gene of the melanin-biosynthesis gene cluster (alb1) in P. marneffei was knocked down, which was accompanied by loss of melanin pigment production and reduced ornamentation in conidia. The survival of mice challenged with the alb1 knockdown mutant was significantly better than those challenged with wild-type P. marneffei (P < 0.005). The sterilizing doses of hydrogen peroxide, leading to a 50% reduction in survival of conidia, were 11 min for wild-type P. marneffei and 6 min for the alb1 knockdown mutant of P. marneffei, implying that the melanin-biosynthesis gene cluster contributed to virulence through decreased susceptibility to killing by hydrogen peroxide. © 2010 FEBS.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.febsjournal.org/en_US
dc.relation.ispartofFEBS Journalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDisinfectants - Pharmacologyen_US
dc.subject.meshFungal Proteins - Geneticsen_US
dc.subject.meshGene Silencingen_US
dc.subject.meshGenes, Fungalen_US
dc.subject.meshInhibitory Concentration 50en_US
dc.subject.meshMelanins - Biosynthesisen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshMicrobial Viability - Drug Effectsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMultigene Family - Geneticsen_US
dc.subject.meshMycoses - Mortalityen_US
dc.subject.meshPenicillium - Drug Effects - Genetics - Pathogenicity - Radiation Effectsen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPolyketide Synthases - Geneticsen_US
dc.subject.meshSpores, Fungal - Drug Effects - Genetics - Radiation Effects - Ultrastructureen_US
dc.subject.meshSurvival Analysisen_US
dc.subject.meshUltraviolet Raysen_US
dc.titleHigh diversity of polyketide synthase genes and the melanin biosynthesis gene cluster in Penicillium marneffeien_US
dc.typeArticleen_US
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_US
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1742-4658.2010.07776.xen_US
dc.identifier.pmid20718860-
dc.identifier.scopuseid_2-s2.0-77956313512en_US
dc.identifier.hkuros182362-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956313512&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume277en_US
dc.identifier.issue18en_US
dc.identifier.spage3750en_US
dc.identifier.epage3758en_US
dc.identifier.isiWOS:000281555600012-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridTam, EWT=37027319900en_US
dc.identifier.scopusauthoridChong, KTK=7102553965en_US
dc.identifier.scopusauthoridCai, JJ=7403153560en_US
dc.identifier.scopusauthoridTung, ETK=23398349800en_US
dc.identifier.scopusauthoridNgan, AHY=14037517900en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats