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Article: Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
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TitleLeptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
 
AuthorsWoo, PCY1
Wong, SSY1
Teng, JLL1
Leung, KW1
Ngan, AHY1
Zhao, DQ1
Tse, H1
Lau, SKP1
Yuen, KY1
 
Issue Date2010
 
PublisherZheijiang University Press. The Journal's web site is located at http://www.zju.edu.cn/jzus/
 
CitationJournal Of Zhejiang University: Science B, 2010, v. 11 n. 6, p. 391-401 [How to Cite?]
DOI: http://dx.doi.org/10.1631/jzus.B1000056
 
AbstractA straight, non-sporulating, Gram-variable bacillus (HKU24 T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24 T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO 2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24 T was non-motile and catalase-negative, but positive for alkaline phosphatase, ß-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24 T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24 T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010.
 
ISSN1673-1581
2013 Impact Factor: 1.293
2013 SCImago Journal Rankings: 0.457
 
DOIhttp://dx.doi.org/10.1631/jzus.B1000056
 
ISI Accession Number IDWOS:000278156000001
Funding AgencyGrant Number
Hong Kong Special Administrative Region of China
Research Grant Council
University Development Fund
Outstanding Young Researcher Award
Committee for Research and Conference Grant
University of Hong Kong, China
Funding Information:

Project partly supported by the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for Department of Health of the Hong Kong Special Administrative Region of China, the Research Grant Council Grant, the University Development Fund, the Outstanding Young Researcher Award, and the Committee for Research and Conference Grant, The University of Hong Kong, China

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWoo, PCY
 
dc.contributor.authorWong, SSY
 
dc.contributor.authorTeng, JLL
 
dc.contributor.authorLeung, KW
 
dc.contributor.authorNgan, AHY
 
dc.contributor.authorZhao, DQ
 
dc.contributor.authorTse, H
 
dc.contributor.authorLau, SKP
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2012-08-08T08:51:32Z
 
dc.date.available2012-08-08T08:51:32Z
 
dc.date.issued2010
 
dc.description.abstractA straight, non-sporulating, Gram-variable bacillus (HKU24 T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24 T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO 2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24 T was non-motile and catalase-negative, but positive for alkaline phosphatase, ß-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24 T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24 T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Zhejiang University: Science B, 2010, v. 11 n. 6, p. 391-401 [How to Cite?]
DOI: http://dx.doi.org/10.1631/jzus.B1000056
 
dc.identifier.citeulike7376255
 
dc.identifier.doihttp://dx.doi.org/10.1631/jzus.B1000056
 
dc.identifier.epage401
 
dc.identifier.isiWOS:000278156000001
Funding AgencyGrant Number
Hong Kong Special Administrative Region of China
Research Grant Council
University Development Fund
Outstanding Young Researcher Award
Committee for Research and Conference Grant
University of Hong Kong, China
Funding Information:

Project partly supported by the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for Department of Health of the Hong Kong Special Administrative Region of China, the Research Grant Council Grant, the University Development Fund, the Outstanding Young Researcher Award, and the Committee for Research and Conference Grant, The University of Hong Kong, China

 
dc.identifier.issn1673-1581
2013 Impact Factor: 1.293
2013 SCImago Journal Rankings: 0.457
 
dc.identifier.issue6
 
dc.identifier.pmid20506569
 
dc.identifier.scopuseid_2-s2.0-77954682796
 
dc.identifier.spage391
 
dc.identifier.urihttp://hdl.handle.net/10722/157592
 
dc.identifier.volume11
 
dc.languageeng
 
dc.publisherZheijiang University Press. The Journal's web site is located at http://www.zju.edu.cn/jzus/
 
dc.publisher.placeChina
 
dc.relation.ispartofJournal of Zhejiang University: Science B
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAged
 
dc.subject.meshBase Sequence
 
dc.subject.meshDna, Bacterial - Genetics
 
dc.subject.meshFemale
 
dc.subject.meshHumans
 
dc.subject.meshLeptotrichia - Classification - Genetics - Isolation & Purification
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshMouth - Microbiology
 
dc.subject.meshSpecies Specificity
 
dc.titleLeptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong