Article: Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir

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TitleLeptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
AuthorsWoo, PCY1
Wong, SSY1
Teng, JLL1
Leung, KW1
Ngan, AHY1
Zhao, DQ1
Tse, H1
Lau, SKP1
Yuen, KY1
Issue Date2010
PublisherZheijiang University Press. The Journal's web site is located at http://www.zju.edu.cn/jzus/
CitationJournal Of Zhejiang University: Science B, 2010, v. 11 n. 6, p. 391-401 [How to Cite?]
DOI: http://dx.doi.org/10.1631/jzus.B1000056
AbstractA straight, non-sporulating, Gram-variable bacillus (HKU24 T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24 T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO 2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24 T was non-motile and catalase-negative, but positive for alkaline phosphatase, ß-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24 T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24 T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010.
ISSN1673-1581
2011 Impact Factor: 1.099
2011 SCImago Journal Rankings: 0.152
DOIhttp://dx.doi.org/10.1631/jzus.B1000056
ISI Accession Number IDWOS:000278156000001
Funding AgencyGrant Number
Hong Kong Special Administrative Region of China
Research Grant Council
University Development Fund
Outstanding Young Researcher Award
Committee for Research and Conference Grant
University of Hong Kong, China
Funding Information:

Project partly supported by the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for Department of Health of the Hong Kong Special Administrative Region of China, the Research Grant Council Grant, the University Development Fund, the Outstanding Young Researcher Award, and the Committee for Research and Conference Grant, The University of Hong Kong, China

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWoo, PCY
dc.contributor.authorWong, SSY
dc.contributor.authorTeng, JLL
dc.contributor.authorLeung, KW
dc.contributor.authorNgan, AHY
dc.contributor.authorZhao, DQ
dc.contributor.authorTse, H
dc.contributor.authorLau, SKP
dc.contributor.authorYuen, KY
dc.date.accessioned2012-08-08T08:51:32Z
dc.date.available2012-08-08T08:51:32Z
dc.date.issued2010
dc.description.abstractA straight, non-sporulating, Gram-variable bacillus (HKU24 T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24 T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO 2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24 T was non-motile and catalase-negative, but positive for alkaline phosphatase, ß-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24 T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24 T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Zhejiang University: Science B, 2010, v. 11 n. 6, p. 391-401 [How to Cite?]
DOI: http://dx.doi.org/10.1631/jzus.B1000056
dc.identifier.citeulike7376255
dc.identifier.doihttp://dx.doi.org/10.1631/jzus.B1000056
dc.identifier.epage401
dc.identifier.isiWOS:000278156000001
Funding AgencyGrant Number
Hong Kong Special Administrative Region of China
Research Grant Council
University Development Fund
Outstanding Young Researcher Award
Committee for Research and Conference Grant
University of Hong Kong, China
Funding Information:

Project partly supported by the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for Department of Health of the Hong Kong Special Administrative Region of China, the Research Grant Council Grant, the University Development Fund, the Outstanding Young Researcher Award, and the Committee for Research and Conference Grant, The University of Hong Kong, China

dc.identifier.issn1673-1581
2011 Impact Factor: 1.099
2011 SCImago Journal Rankings: 0.152
dc.identifier.issue6
dc.identifier.pmid20506569
dc.identifier.scopuseid_2-s2.0-77954682796
dc.identifier.spage391
dc.identifier.urihttp://hdl.handle.net/10722/157592
dc.identifier.volume11
dc.languageeng
dc.publisherZheijiang University Press. The Journal's web site is located at http://www.zju.edu.cn/jzus/
dc.publisher.placeChina
dc.relation.ispartofJournal of Zhejiang University: Science B
dc.relation.referencesReferences in Scopus
dc.subject.meshAged
dc.subject.meshBase Sequence
dc.subject.meshDna, Bacterial - Genetics
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshLeptotrichia - Classification - Genetics - Isolation & Purification
dc.subject.meshMolecular Sequence Data
dc.subject.meshMouth - Microbiology
dc.subject.meshSpecies Specificity
dc.titleLeptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong