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Article: Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir

TitleLeptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
Authors
Issue Date2010
PublisherZheijiang University Press. The Journal's web site is located at http://www.zju.edu.cn/jzus/
Citation
Journal Of Zhejiang University: Science B, 2010, v. 11 n. 6, p. 391-401 How to Cite?
AbstractA straight, non-sporulating, Gram-variable bacillus (HKU24 T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24 T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO 2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24 T was non-motile and catalase-negative, but positive for alkaline phosphatase, ß-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24 T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24 T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010.
Persistent Identifierhttp://hdl.handle.net/10722/157592
ISSN
2014 Impact Factor: 1.278
2014 SCImago Journal Rankings: 0.429
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Special Administrative Region of China
Research Grant Council
University Development Fund
Outstanding Young Researcher Award
Committee for Research and Conference Grant
University of Hong Kong, China
Funding Information:

Project partly supported by the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for Department of Health of the Hong Kong Special Administrative Region of China, the Research Grant Council Grant, the University Development Fund, the Outstanding Young Researcher Award, and the Committee for Research and Conference Grant, The University of Hong Kong, China

References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorWong, SSYen_US
dc.contributor.authorTeng, JLLen_US
dc.contributor.authorLeung, KWen_US
dc.contributor.authorNgan, AHYen_US
dc.contributor.authorZhao, DQen_US
dc.contributor.authorTse, Hen_US
dc.contributor.authorLau, SKPen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:51:32Z-
dc.date.available2012-08-08T08:51:32Z-
dc.date.issued2010en_US
dc.identifier.citationJournal Of Zhejiang University: Science B, 2010, v. 11 n. 6, p. 391-401en_US
dc.identifier.issn1673-1581en_US
dc.identifier.urihttp://hdl.handle.net/10722/157592-
dc.description.abstractA straight, non-sporulating, Gram-variable bacillus (HKU24 T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24 T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO 2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24 T was non-motile and catalase-negative, but positive for alkaline phosphatase, ß-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24 T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24 T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010.en_US
dc.languageengen_US
dc.publisherZheijiang University Press. The Journal's web site is located at http://www.zju.edu.cn/jzus/en_US
dc.relation.ispartofJournal of Zhejiang University: Science Ben_US
dc.subject.meshAgeden_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDna, Bacterial - Geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLeptotrichia - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMouth - Microbiologyen_US
dc.subject.meshSpecies Specificityen_US
dc.titleLeptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoiren_US
dc.typeArticleen_US
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_US
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_US
dc.identifier.emailTeng, JLL:llteng@hku.hken_US
dc.identifier.emailTse, H:herman@graduate.hku.hken_US
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityWong, SSY=rp00395en_US
dc.identifier.authorityTeng, JLL=rp00277en_US
dc.identifier.authorityTse, H=rp00519en_US
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1631/jzus.B1000056en_US
dc.identifier.pmid20506569-
dc.identifier.scopuseid_2-s2.0-77954682796en_US
dc.identifier.hkuros171985-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954682796&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume11en_US
dc.identifier.issue6en_US
dc.identifier.spage391en_US
dc.identifier.epage401en_US
dc.identifier.isiWOS:000278156000001-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridWong, SSY=13310021400en_US
dc.identifier.scopusauthoridTeng, JLL=7202560229en_US
dc.identifier.scopusauthoridLeung, KW=7401860831en_US
dc.identifier.scopusauthoridNgan, AHY=14037517900en_US
dc.identifier.scopusauthoridZhao, DQ=7403489840en_US
dc.identifier.scopusauthoridTse, H=7006070596en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.citeulike7376255-

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