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Article: Genetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources
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TitleGenetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources
 
AuthorsHo, PL1
Wong, RC2
Lo, SW1
Chow, KH1
Wong, SS1
Que, TL2
 
Issue Date2010
 
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
 
CitationJournal Of Medical Microbiology, 2010, v. 59 n. 6, p. 702-707 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.015032-0
 
AbstractA bacterial collection (n=249) obtained in Hong Kong from 2002 to 2004 was used to investigate the molecular epidemiology of aminoglycoside resistance among Escherichia coli isolates from humans and food-producing animals. Of these, 89 isolates were gentamicin-sensitive (human n=60, animal n=29) and 160 isolates were gentamicin-resistant (human n=107, animal n=53). Overall, 84.1% (90/107) and 75.5% (40/53) of the gentamicin-resistant isolates from human and animal sources, respectively, were found to possess the aacC2 gene. The aacC2 gene for 20 isolates (10 each for human and animal isolates) was sequenced. Two alleles were found that were equally distributed in human and animal isolates. PFGE showed that the gentamicin-resistant isolates exhibited diverse patterns with little clonality. In some isolates, the aacC2 gene was encoded on large transferable plasmids of multiple incompatibility groups (IncF, IncI1 and IncN). An IncFII plasmid of 140 kb in size was shared by one human and three animal isolates. In summary, this study showed that human and animal isolates share the same pool of resistance genes. © 2010 SGM.
 
ISSN0022-2615
2012 Impact Factor: 2.297
2012 SCImago Journal Rankings: 0.886
 
DOIhttp://dx.doi.org/10.1099/jmm.0.015032-0
 
ISI Accession Number IDWOS:000278702000012
Funding AgencyGrant Number
Research Grant CouncilHKU 7513/06M
University Development Fund
University of I long Kong
Research Fund for the Control of Infectious Diseases (RFCID) of the Health
Welfare and Food Bureau of the I-long Kong SAR Government
Funding Information:

This work was supported by research grants from the Research Grant Council (HKU 7513/06M) and the University Development Fund Project - Research Centre of Emerging Infectious Diseases, the University of I long Kong, and the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the I-long Kong SAR Government We thank the University of Hong Kong for the Outstanding Research Postgraduate Student award to River C Wong

 
ReferencesReferences in Scopus
 
GrantsThe epidemiological relationships between CTX-M producing Escherichia coli from animal and human sources
 
DC FieldValue
dc.contributor.authorHo, PL
 
dc.contributor.authorWong, RC
 
dc.contributor.authorLo, SW
 
dc.contributor.authorChow, KH
 
dc.contributor.authorWong, SS
 
dc.contributor.authorQue, TL
 
dc.date.accessioned2012-08-08T08:51:30Z
 
dc.date.available2012-08-08T08:51:30Z
 
dc.date.issued2010
 
dc.description.abstractA bacterial collection (n=249) obtained in Hong Kong from 2002 to 2004 was used to investigate the molecular epidemiology of aminoglycoside resistance among Escherichia coli isolates from humans and food-producing animals. Of these, 89 isolates were gentamicin-sensitive (human n=60, animal n=29) and 160 isolates were gentamicin-resistant (human n=107, animal n=53). Overall, 84.1% (90/107) and 75.5% (40/53) of the gentamicin-resistant isolates from human and animal sources, respectively, were found to possess the aacC2 gene. The aacC2 gene for 20 isolates (10 each for human and animal isolates) was sequenced. Two alleles were found that were equally distributed in human and animal isolates. PFGE showed that the gentamicin-resistant isolates exhibited diverse patterns with little clonality. In some isolates, the aacC2 gene was encoded on large transferable plasmids of multiple incompatibility groups (IncF, IncI1 and IncN). An IncFII plasmid of 140 kb in size was shared by one human and three animal isolates. In summary, this study showed that human and animal isolates share the same pool of resistance genes. © 2010 SGM.
 
dc.description.natureLink_to_OA_fulltext
 
dc.identifier.citationJournal Of Medical Microbiology, 2010, v. 59 n. 6, p. 702-707 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.015032-0
 
dc.identifier.doihttp://dx.doi.org/10.1099/jmm.0.015032-0
 
dc.identifier.epage707
 
dc.identifier.hkuros219373
 
dc.identifier.isiWOS:000278702000012
Funding AgencyGrant Number
Research Grant CouncilHKU 7513/06M
University Development Fund
University of I long Kong
Research Fund for the Control of Infectious Diseases (RFCID) of the Health
Welfare and Food Bureau of the I-long Kong SAR Government
Funding Information:

This work was supported by research grants from the Research Grant Council (HKU 7513/06M) and the University Development Fund Project - Research Centre of Emerging Infectious Diseases, the University of I long Kong, and the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the I-long Kong SAR Government We thank the University of Hong Kong for the Outstanding Research Postgraduate Student award to River C Wong

 
dc.identifier.issn0022-2615
2012 Impact Factor: 2.297
2012 SCImago Journal Rankings: 0.886
 
dc.identifier.issue6
 
dc.identifier.scopuseid_2-s2.0-77952997850
 
dc.identifier.spage702
 
dc.identifier.urihttp://hdl.handle.net/10722/157589
 
dc.identifier.volume59
 
dc.languageeng
 
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Medical Microbiology
 
dc.relation.projectThe epidemiological relationships between CTX-M producing Escherichia coli from animal and human sources
 
dc.relation.referencesReferences in Scopus
 
dc.titleGenetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Tuen Mun Hospital