Article: Genetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources
| Title | Genetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Authors | Ho, PL2 Wong, RC1 Lo, SW2 Chow, KH2 Wong, SS2 Que, TL1 | ||||||||||||
| Issue Date | 2010 | ||||||||||||
| Publisher | Society for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org | ||||||||||||
| Citation | Journal Of Medical Microbiology, 2010, v. 59 n. 6, p. 702-707 [How to Cite?] DOI: http://dx.doi.org/10.1099/jmm.0.015032-0 | ||||||||||||
| Abstract | A bacterial collection (n=249) obtained in Hong Kong from 2002 to 2004 was used to investigate the molecular epidemiology of aminoglycoside resistance among Escherichia coli isolates from humans and food-producing animals. Of these, 89 isolates were gentamicin-sensitive (human n=60, animal n=29) and 160 isolates were gentamicin-resistant (human n=107, animal n=53). Overall, 84.1% (90/107) and 75.5% (40/53) of the gentamicin-resistant isolates from human and animal sources, respectively, were found to possess the aacC2 gene. The aacC2 gene for 20 isolates (10 each for human and animal isolates) was sequenced. Two alleles were found that were equally distributed in human and animal isolates. PFGE showed that the gentamicin-resistant isolates exhibited diverse patterns with little clonality. In some isolates, the aacC2 gene was encoded on large transferable plasmids of multiple incompatibility groups (IncF, IncI1 and IncN). An IncFII plasmid of 140 kb in size was shared by one human and three animal isolates. In summary, this study showed that human and animal isolates share the same pool of resistance genes. © 2010 SGM. | ||||||||||||
| ISSN | 0022-2615 2011 Impact Factor: 2.502 2011 SCImago Journal Rankings: 0.222 | ||||||||||||
| DOI | http://dx.doi.org/10.1099/jmm.0.015032-0 | ||||||||||||
| ISI Accession Number ID | WOS:000278702000012
Funding Information: This work was supported by research grants from the Research Grant Council (HKU 7513/06M) and the University Development Fund Project - Research Centre of Emerging Infectious Diseases, the University of I long Kong, and the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the I-long Kong SAR Government We thank the University of Hong Kong for the Outstanding Research Postgraduate Student award to River C Wong | ||||||||||||
| References | References in Scopus | ||||||||||||
| Grants | The epidemiological relationships between CTX-M producing Escherichia coli from animal and human sources |
| dc.contributor.author | Ho, PL | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Wong, RC | ||||||||||||
| dc.contributor.author | Lo, SW | ||||||||||||
| dc.contributor.author | Chow, KH | ||||||||||||
| dc.contributor.author | Wong, SS | ||||||||||||
| dc.contributor.author | Que, TL | ||||||||||||
| dc.date.accessioned | 2012-08-08T08:51:30Z | ||||||||||||
| dc.date.available | 2012-08-08T08:51:30Z | ||||||||||||
| dc.date.issued | 2010 | ||||||||||||
| dc.description.abstract | A bacterial collection (n=249) obtained in Hong Kong from 2002 to 2004 was used to investigate the molecular epidemiology of aminoglycoside resistance among Escherichia coli isolates from humans and food-producing animals. Of these, 89 isolates were gentamicin-sensitive (human n=60, animal n=29) and 160 isolates were gentamicin-resistant (human n=107, animal n=53). Overall, 84.1% (90/107) and 75.5% (40/53) of the gentamicin-resistant isolates from human and animal sources, respectively, were found to possess the aacC2 gene. The aacC2 gene for 20 isolates (10 each for human and animal isolates) was sequenced. Two alleles were found that were equally distributed in human and animal isolates. PFGE showed that the gentamicin-resistant isolates exhibited diverse patterns with little clonality. In some isolates, the aacC2 gene was encoded on large transferable plasmids of multiple incompatibility groups (IncF, IncI1 and IncN). An IncFII plasmid of 140 kb in size was shared by one human and three animal isolates. In summary, this study showed that human and animal isolates share the same pool of resistance genes. © 2010 SGM. | ||||||||||||
| dc.description.grant | The epidemiological relationships between CTX-M producing Escherichia coli from animal and human sources | ||||||||||||
| dc.description.grantcode | 82404 | ||||||||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||||||||
| dc.identifier.citation | Journal Of Medical Microbiology, 2010, v. 59 n. 6, p. 702-707 [How to Cite?] DOI: http://dx.doi.org/10.1099/jmm.0.015032-0 | ||||||||||||
| dc.identifier.doi | http://dx.doi.org/10.1099/jmm.0.015032-0 | ||||||||||||
| dc.identifier.epage | 707 | ||||||||||||
| dc.identifier.isi | WOS:000278702000012
Funding Information: This work was supported by research grants from the Research Grant Council (HKU 7513/06M) and the University Development Fund Project - Research Centre of Emerging Infectious Diseases, the University of I long Kong, and the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the I-long Kong SAR Government We thank the University of Hong Kong for the Outstanding Research Postgraduate Student award to River C Wong | ||||||||||||
| dc.identifier.issn | 0022-2615 2011 Impact Factor: 2.502 2011 SCImago Journal Rankings: 0.222 | ||||||||||||
| dc.identifier.issue | 6 | ||||||||||||
| dc.identifier.scopus | eid_2-s2.0-77952997850 | ||||||||||||
| dc.identifier.spage | 702 | ||||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/157589 | ||||||||||||
| dc.identifier.volume | 59 | ||||||||||||
| dc.language | eng | ||||||||||||
| dc.publisher | Society for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org | ||||||||||||
| dc.publisher.place | United Kingdom | ||||||||||||
| dc.relation.ispartof | Journal of Medical Microbiology | ||||||||||||
| dc.relation.references | References in Scopus | ||||||||||||
| dc.title | Genetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources | ||||||||||||
| dc.type | Article |
Author Affiliations
- Tuen Mun Hospital
- The University of Hong Kong