File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Epidemiology and clonality of multidrug-resistant Acinetobacter baumannii from a healthcare region in Hong Kong.

TitleEpidemiology and clonality of multidrug-resistant Acinetobacter baumannii from a healthcare region in Hong Kong.
Authors
KeywordsAcinetobacter Baumannii
Molecular Epidemiology
Multilocus Sequence Typing
Risk Factors
Issue Date2010
Citation
The Journal Of Hospital Infection, 2010, v. 74 n. 4, p. 358-364 How to Cite?
AbstractWe assessed the risk factors and molecular epidemiology of multidrug-resistant Acinetobacter baumannii (MDR-AB) in Hong Kong. The patients were treated in five hospitals in a healthcare region during 2005-2006. We performed genomic identification by amplified rRNA gene restriction analysis (ARDRA) and investigated the existence of metallo-beta-lactamases and the clonality of representative MDR-AB strains by phenotypic and molecular methods. Forty-five subjects with MDR-AB were compared with 135 controls (patients with no MDR-AB). In the logistic regression, chronic wound (odds ratio: 29.5, 95% confidence interval: 8.1-107.2; P<0.001) was the only factor independently associated with MDR-AB colonisation or infection. ARDRA identified all 45 MDR-AB as genomic species 2TU. Pulsed-field gel electrophoresis clustered all except two isolates into two clonal types, designated HKU1 and HKU2 with 24 and 19 isolates, respectively. The main features of HKU1 strains were ST26, adeB type XII, positivity for bla(OxA-23-like) and bla(OxA-51-like) genes and high level resistance to carbapenems. Most HKU1 strains retained susceptibility to gentamicin, cotrimoxazole and minocycline. By contrast, HKU2 strains exhibited ST22, adeB type II, and were usually positive only for the bla(OxA-51-like) gene and resistant to gentamicin, cotrimoxazole and minocycline. Both clones were found to have disseminated widely. In conclusion, clonal expansion is playing major roles in the increase of MDR-AB in these hospitals in Hong Kong. The findings highlight the need to enhance infection control measures. Copyright (c) 2009 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157587
ISSN
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Hong Kong SAR Government
Funding Information:

This work was supported by grants from the University Development Fund Project-Research Centre of Emerging Infectious Diseases of the University of Hong Kong and the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Hong Kong SAR Government.

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_US
dc.contributor.authorHo, AYen_US
dc.contributor.authorChow, KHen_US
dc.contributor.authorLai, ELen_US
dc.contributor.authorChing, Pen_US
dc.contributor.authorSeto, WHen_US
dc.date.accessioned2012-08-08T08:51:29Z-
dc.date.available2012-08-08T08:51:29Z-
dc.date.issued2010en_US
dc.identifier.citationThe Journal Of Hospital Infection, 2010, v. 74 n. 4, p. 358-364en_US
dc.identifier.issn1532-2939en_US
dc.identifier.urihttp://hdl.handle.net/10722/157587-
dc.description.abstractWe assessed the risk factors and molecular epidemiology of multidrug-resistant Acinetobacter baumannii (MDR-AB) in Hong Kong. The patients were treated in five hospitals in a healthcare region during 2005-2006. We performed genomic identification by amplified rRNA gene restriction analysis (ARDRA) and investigated the existence of metallo-beta-lactamases and the clonality of representative MDR-AB strains by phenotypic and molecular methods. Forty-five subjects with MDR-AB were compared with 135 controls (patients with no MDR-AB). In the logistic regression, chronic wound (odds ratio: 29.5, 95% confidence interval: 8.1-107.2; P<0.001) was the only factor independently associated with MDR-AB colonisation or infection. ARDRA identified all 45 MDR-AB as genomic species 2TU. Pulsed-field gel electrophoresis clustered all except two isolates into two clonal types, designated HKU1 and HKU2 with 24 and 19 isolates, respectively. The main features of HKU1 strains were ST26, adeB type XII, positivity for bla(OxA-23-like) and bla(OxA-51-like) genes and high level resistance to carbapenems. Most HKU1 strains retained susceptibility to gentamicin, cotrimoxazole and minocycline. By contrast, HKU2 strains exhibited ST22, adeB type II, and were usually positive only for the bla(OxA-51-like) gene and resistant to gentamicin, cotrimoxazole and minocycline. Both clones were found to have disseminated widely. In conclusion, clonal expansion is playing major roles in the increase of MDR-AB in these hospitals in Hong Kong. The findings highlight the need to enhance infection control measures. Copyright (c) 2009 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.relation.ispartofThe Journal of hospital infectionen_US
dc.subjectAcinetobacter Baumannii-
dc.subjectMolecular Epidemiology-
dc.subjectMultilocus Sequence Typing-
dc.subjectRisk Factors-
dc.subject.meshAcinetobacter Infections - Epidemiology - Microbiologyen_US
dc.subject.meshAcinetobacter Baumannii - Classification - Drug Effects - Isolation & Purificationen_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAnti-Bacterial Agents - Pharmacologyen_US
dc.subject.meshBacterial Typing Techniquesen_US
dc.subject.meshCluster Analysisen_US
dc.subject.meshCross Infection - Epidemiology - Microbiologyen_US
dc.subject.meshDna, Bacterial - Chemistry - Geneticsen_US
dc.subject.meshDrug Resistance, Multiple, Bacterialen_US
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden_US
dc.subject.meshFemaleen_US
dc.subject.meshHealth Facilitiesen_US
dc.subject.meshHong Kong - Epidemiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshRisk Factorsen_US
dc.subject.meshWound Infection - Epidemiology - Microbiologyen_US
dc.titleEpidemiology and clonality of multidrug-resistant Acinetobacter baumannii from a healthcare region in Hong Kong.en_US
dc.typeArticleen_US
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_US
dc.identifier.emailChow, KH:khchowb@hku.hken_US
dc.identifier.authorityHo, PL=rp00406en_US
dc.identifier.authorityChow, KH=rp00370en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jhin.2009.10.015-
dc.identifier.pmid20153548en_US
dc.identifier.scopuseid_2-s2.0-77952580158en_US
dc.identifier.volume74en_US
dc.identifier.issue4en_US
dc.identifier.spage358en_US
dc.identifier.epage364en_US
dc.identifier.isiWOS:000276456400008-
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridHo, AY=7402675209en_US
dc.identifier.scopusauthoridChow, KH=7202180736en_US
dc.identifier.scopusauthoridLai, EL=8238477100en_US
dc.identifier.scopusauthoridChing, P=7005847968en_US
dc.identifier.scopusauthoridSeto, WH=7005799377en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats