File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Detection of asymptomatic antigenemia in pigs infected by porcine reproductive and respiratory syndrome virus (PRRSV) by a novel capture immunoassay with monoclonal antibodies against the nucleocapsid protein of PRRSV

TitleDetection of asymptomatic antigenemia in pigs infected by porcine reproductive and respiratory syndrome virus (PRRSV) by a novel capture immunoassay with monoclonal antibodies against the nucleocapsid protein of PRRSV
Authors
Issue Date2009
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
Citation
Clinical And Vaccine Immunology, 2009, v. 16 n. 12, p. 1822-1828 How to Cite?
AbstractRoutine surveillance for porcine reproductive and respiratory syndrome virus (PRRSV) infections is crucial for the epidemiological control of this disease. Antibody tests are widely used but cannot differentiate between vaccination and reinfection. We developed a PRRSV antigen capture enzyme-linked immunosorbent assay (ELISA) using well-characterized monoclonal antibodies (MAbs) raised against the nucleocapsid (N) protein of North American and European PRRSV. This antigen assay detected purified N protein from both genotypes at levels as low as 0.4 and 0.8 ng, respectively. The specificity and sensitivity of the N antigen assay were evaluated with ground lung tissues from 8 PRRSV-infected and 16 healthy swine, and culture supernatants from six PRRSV isolates as well as other swine viruses were confirmed by reverse transcriptase PCR (RTPCR). Antigen assays were positive in all eight infected tissues and with six different PRRSV isolates, with no false positives among healthy tissues and other swine viruses (i.e., pseudorabies and foot and mouth disease viruses). A number of sera, field collected from 466 vaccinated and asymptomatic pigs in Guangdong, China, between 2008 and 2009, tested positive by the N antigen assay (12.45%), RT-PCR (15.02%), and a commercial test for antibodies against PRRSV (78.97%). Of the 466 sera, 47 were positive by both antigen and RT-PCR tests, 11 by antigen test only, and 23 by RT-PCR only; the two assays had an overall agreement of 92.7%, indicating a significant percentage of active PRRSV in asymptomatic pigs despite previous immunization. These findings suggest that the antigen assay is a valuable field tool for the epidemiological control of PRRSV that can be used for rapid screening, particularly in asymptomatic animals. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157574
ISSN
2015 Impact Factor: 2.277
2015 SCImago Journal Rankings: 1.329
ISI Accession Number ID
Funding AgencyGrant Number
National Outstanding Young Scientist Foundation of China30725031
National Science and Technology Major Project of China2009ZX10004-306
Funding Information:

This work was supported by grant 30725031 of the National Outstanding Young Scientist Foundation of China and grant 2009ZX10004-306 of National Science and Technology Major Project of China.

References

 

DC FieldValueLanguage
dc.contributor.authorCai, JPen_US
dc.contributor.authorWang, YDen_US
dc.contributor.authorTse, Hen_US
dc.contributor.authorXiang, Hen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorChe, XYen_US
dc.date.accessioned2012-08-08T08:51:23Z-
dc.date.available2012-08-08T08:51:23Z-
dc.date.issued2009en_US
dc.identifier.citationClinical And Vaccine Immunology, 2009, v. 16 n. 12, p. 1822-1828en_US
dc.identifier.issn1556-6811en_US
dc.identifier.urihttp://hdl.handle.net/10722/157574-
dc.description.abstractRoutine surveillance for porcine reproductive and respiratory syndrome virus (PRRSV) infections is crucial for the epidemiological control of this disease. Antibody tests are widely used but cannot differentiate between vaccination and reinfection. We developed a PRRSV antigen capture enzyme-linked immunosorbent assay (ELISA) using well-characterized monoclonal antibodies (MAbs) raised against the nucleocapsid (N) protein of North American and European PRRSV. This antigen assay detected purified N protein from both genotypes at levels as low as 0.4 and 0.8 ng, respectively. The specificity and sensitivity of the N antigen assay were evaluated with ground lung tissues from 8 PRRSV-infected and 16 healthy swine, and culture supernatants from six PRRSV isolates as well as other swine viruses were confirmed by reverse transcriptase PCR (RTPCR). Antigen assays were positive in all eight infected tissues and with six different PRRSV isolates, with no false positives among healthy tissues and other swine viruses (i.e., pseudorabies and foot and mouth disease viruses). A number of sera, field collected from 466 vaccinated and asymptomatic pigs in Guangdong, China, between 2008 and 2009, tested positive by the N antigen assay (12.45%), RT-PCR (15.02%), and a commercial test for antibodies against PRRSV (78.97%). Of the 466 sera, 47 were positive by both antigen and RT-PCR tests, 11 by antigen test only, and 23 by RT-PCR only; the two assays had an overall agreement of 92.7%, indicating a significant percentage of active PRRSV in asymptomatic pigs despite previous immunization. These findings suggest that the antigen assay is a valuable field tool for the epidemiological control of PRRSV that can be used for rapid screening, particularly in asymptomatic animals. Copyright © 2009, American Society for Microbiology. All Rights Reserved.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/en_US
dc.relation.ispartofClinical and Vaccine Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshAntigens, Viral - Analysis - Immunologyen_US
dc.subject.meshChina - Epidemiologyen_US
dc.subject.meshEnvironmental Monitoring - Methodsen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshLung - Immunology - Pathology - Virologyen_US
dc.subject.meshNucleocapsid Proteins - Analysis - Immunologyen_US
dc.subject.meshPorcine Reproductive And Respiratory Syndrome - Diagnosis - Epidemiology - Immunologyen_US
dc.subject.meshPorcine Respiratory And Reproductive Syndrome Virus - Immunology - Isolation & Purificationen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSwineen_US
dc.titleDetection of asymptomatic antigenemia in pigs infected by porcine reproductive and respiratory syndrome virus (PRRSV) by a novel capture immunoassay with monoclonal antibodies against the nucleocapsid protein of PRRSVen_US
dc.typeArticleen_US
dc.identifier.emailTse, H:herman@graduate.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityTse, H=rp00519en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/CVI.00244-09en_US
dc.identifier.pmid19828768-
dc.identifier.scopuseid_2-s2.0-73449137222en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73449137222&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume16en_US
dc.identifier.issue12en_US
dc.identifier.spage1822en_US
dc.identifier.epage1828en_US
dc.identifier.isiWOS:000272157200015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCai, JP=35557916700en_US
dc.identifier.scopusauthoridWang, YD=9638471800en_US
dc.identifier.scopusauthoridTse, H=7006070596en_US
dc.identifier.scopusauthoridXiang, H=35559854400en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridChe, XY=7005743182en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats