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Article: Fine antigenic variation within H5N1 influenza virus hemagglutinin's antigenic sites defined by yeast cell surface display

TitleFine antigenic variation within H5N1 influenza virus hemagglutinin's antigenic sites defined by yeast cell surface display
Authors
Issue Date2009
PublisherWiley - VCH Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal of Immunology, 2009, v. 39 n. 12, p. 3498-3510 How to Cite?
AbstractFifteen strains of mAb specific for HA of the A/Hong Kong/482/97 (H5N1) influenza virus were generated. The HA antigenic sites of the human A/Hong Kong/482/97 (H5N1) influenza virus were defined by using yeast cell surface-displaying system and anti-H5 HA mAb. Evolution analysis of H5 HA identified residues that exhibit diversifying selection in the antigenic sites and demonstrated surprising differences between residue variation of H5 HA and H3 HA. A conserved neutralizing epitope in the H5 HA protein recognized by mAb H5M9 was found using viruses isolated from 1997-2006. Seven single amino acid substitutions were introduced into the HA antigenic sites, respectively, and the alteration of antigenicity was assessed. The structure obtained by homology-modeling and molecular dynamic methods showed that a subtle substitution at residue 124 propagates throughout its nearby loop (152-159). We discuss how the structural changes caused by point mutation might explain the altered antigenicity of the HA protein. The results demonstrate the existence of immunodominant positions in the H5 HA protein, alteration of these residues might improve the immunogenicity of vaccine strains. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/157571
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References
Errata

 

DC FieldValueLanguage
dc.contributor.authorLi, Jen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorLiang, Yen_US
dc.contributor.authorNi, Ben_US
dc.contributor.authorWan, Yen_US
dc.contributor.authorLiao, Zen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorFu, Xen_US
dc.contributor.authorShang, Xen_US
dc.contributor.authorWang, Sen_US
dc.contributor.authorYi, Den_US
dc.contributor.authorGuo, Ben_US
dc.contributor.authorDi, Ben_US
dc.contributor.authorWang, Men_US
dc.contributor.authorChe, Xen_US
dc.contributor.authorWu, Yen_US
dc.date.accessioned2012-08-08T08:51:22Z-
dc.date.available2012-08-08T08:51:22Z-
dc.date.issued2009en_US
dc.identifier.citationEuropean Journal of Immunology, 2009, v. 39 n. 12, p. 3498-3510en_US
dc.identifier.issn0014-2980en_US
dc.identifier.urihttp://hdl.handle.net/10722/157571-
dc.description.abstractFifteen strains of mAb specific for HA of the A/Hong Kong/482/97 (H5N1) influenza virus were generated. The HA antigenic sites of the human A/Hong Kong/482/97 (H5N1) influenza virus were defined by using yeast cell surface-displaying system and anti-H5 HA mAb. Evolution analysis of H5 HA identified residues that exhibit diversifying selection in the antigenic sites and demonstrated surprising differences between residue variation of H5 HA and H3 HA. A conserved neutralizing epitope in the H5 HA protein recognized by mAb H5M9 was found using viruses isolated from 1997-2006. Seven single amino acid substitutions were introduced into the HA antigenic sites, respectively, and the alteration of antigenicity was assessed. The structure obtained by homology-modeling and molecular dynamic methods showed that a subtle substitution at residue 124 propagates throughout its nearby loop (152-159). We discuss how the structural changes caused by point mutation might explain the altered antigenicity of the HA protein. The results demonstrate the existence of immunodominant positions in the H5 HA protein, alteration of these residues might improve the immunogenicity of vaccine strains. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.languageengen_US
dc.publisherWiley - VCH Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_US
dc.relation.ispartofEuropean Journal of Immunologyen_US
dc.subject.meshAmino Acids - Genetics - Immunologyen_US
dc.subject.meshAntibodies, Monoclonal - Genetics - Immunologyen_US
dc.subject.meshAntigenic Variationen_US
dc.subject.meshCell Membrane - Metabolismen_US
dc.subject.meshCrystallography, X-Rayen_US
dc.subject.meshEpitope Mappingen_US
dc.subject.meshEpitopes - Chemistry - Genetics - Immunologyen_US
dc.subject.meshEvolution, Molecularen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - Chemistry - Genetics - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInfluenza A Virus, H5n1 Subtype - Genetics - Immunologyen_US
dc.subject.meshModels, Molecularen_US
dc.subject.meshMutationen_US
dc.subject.meshProtein Conformationen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshYeasts - Genetics - Metabolismen_US
dc.titleFine antigenic variation within H5N1 influenza virus hemagglutinin's antigenic sites defined by yeast cell surface displayen_US
dc.typeArticleen_US
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1002/eji.200939532en_US
dc.identifier.pmid19798682-
dc.identifier.scopuseid_2-s2.0-73249125589en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73249125589&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume39en_US
dc.identifier.issue12en_US
dc.identifier.spage3498en_US
dc.identifier.epage3510en_US
dc.identifier.isiWOS:000272928300026-
dc.publisher.placeGermanyen_US
dc.relation.erratumdoi:10.1002/eji.201090011-
dc.identifier.scopusauthoridLi, J=26643355000en_US
dc.identifier.scopusauthoridWang, Y=9638471800en_US
dc.identifier.scopusauthoridLiang, Y=7403500034en_US
dc.identifier.scopusauthoridNi, B=7101850460en_US
dc.identifier.scopusauthoridWan, Y=8559722000en_US
dc.identifier.scopusauthoridLiao, Z=7203032864en_US
dc.identifier.scopusauthoridChan, KH=14069945400en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridFu, X=34871994400en_US
dc.identifier.scopusauthoridShang, X=23490717300en_US
dc.identifier.scopusauthoridWang, S=7410336908en_US
dc.identifier.scopusauthoridYi, D=55161129800en_US
dc.identifier.scopusauthoridGuo, B=23988351800en_US
dc.identifier.scopusauthoridDi, B=6602190900en_US
dc.identifier.scopusauthoridWang, M=7406685232en_US
dc.identifier.scopusauthoridChe, X=7005743182en_US
dc.identifier.scopusauthoridWu, Y=7406896774en_US
dc.customcontrol.immutablesml 130530-

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