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Article: Differential susceptibility of different cell lines to swine-origin influenza A H1N1, seasonal human influenza A H1N1, and avian influenza A H5N1 viruses

TitleDifferential susceptibility of different cell lines to swine-origin influenza A H1N1, seasonal human influenza A H1N1, and avian influenza A H5N1 viruses
Authors
KeywordsCell tropism
H5N1
Influenza H1N1
Swine-origin
Viral load
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2009, v. 46 n. 4, p. 325-330 How to Cite?
Abstract
Background: The novel swine-origin influenza A H1N1 virus (S-OIV) causes the current pandemic. Its tissue tropism and replication in different cell lines are not well understood. Objective: Compare the growth characteristics of cell lines infected by S-OIV, seasonal influenza A H1N1 (sH1N1) and avian influenza A H5N1 (H5N1) viruses and the effect of temperature on viral replication. Study design: Cytopathic effect (CPE), antigen expression by immunofluorescence (IF) and viral load profile by quantitative RT-PCR in 17 cell lines infected by S-OIV, sH1N1 and H5N1 were examined. Comparison of their replication efficiency in chick embryo was performed. The effect of temperature on viral replication in Madin-Darby canine kidney (MDCK) cells was determined by TCID 50 at 33 °C, 37 °C and 39 °C for 5 consecutive days. Results: S-OIV replicated in cell lines derived from different tissues or organs and host species with comparable viral load to sH1N1. Among 13 human cell lines tested, Caco-2 has the highest viral load for S-OIV. S-OIV showed a low viral load with no CPE or antigen expression in pig kidney cell PK-15, H5N1 demonstrated the most diverse cell tropism by CPE and antigen expression, and the highest viral replication efficiency in both cell lines and allantoic fluid. All three viruses demonstrated best growth at 37 °C in MDCK cells. Conclusion: Cell line growth characteristics of S-OIV, sH1N1 and H5N1 appear to correlate clinically and pathologically with involved anatomical sites and severity. Low replication of S-OIV in PK-15 suggests that this virus is more adapted to human than swine. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157568
ISSN
2013 Impact Factor: 3.466
2013 SCImago Journal Rankings: 1.812
ISI Accession Number ID
Funding AgencyGrant Number
Providence Foundation Limited
University Grant Council
Hong Kong SAR Government
Funding Information:

The authors would like to acknowledge Prof Albert DME Osterhaus from Department of Virology, Erasmus Medical Center, Rotterdam, the Netherlands; the Centers for Disease Control and Prevention, the United States; Prof Fred Leung from Department of Zoology, Prof Pik-To Cheung from Department of Pediatrics, Prof MH Ng who is a past Head of Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, HKSAR; Dr Wilina Lim from Virology Division of Public Health Laboratory Centre, Department of Health, HKSAR, and Dr David Ho from Aaron Diamond AIDS Research Centre, Rockefeller University, for their support in establishment of the cell lines. We would also like to acknowledge Miss Agnes Y. F. Fung and Mr Patrick Lam Y. P. for the technical support. The study is funded by the Providence Foundation Limited in memory of the late Dr Lui Hac Minh, the University Grant Council, and the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Hong Kong SAR Government.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, IWSen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorTo, KWKen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorChan, JFWen_HK
dc.contributor.authorCheng, VCCen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2012-08-08T08:51:20Z-
dc.date.available2012-08-08T08:51:20Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Clinical Virology, 2009, v. 46 n. 4, p. 325-330en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/157568-
dc.description.abstractBackground: The novel swine-origin influenza A H1N1 virus (S-OIV) causes the current pandemic. Its tissue tropism and replication in different cell lines are not well understood. Objective: Compare the growth characteristics of cell lines infected by S-OIV, seasonal influenza A H1N1 (sH1N1) and avian influenza A H5N1 (H5N1) viruses and the effect of temperature on viral replication. Study design: Cytopathic effect (CPE), antigen expression by immunofluorescence (IF) and viral load profile by quantitative RT-PCR in 17 cell lines infected by S-OIV, sH1N1 and H5N1 were examined. Comparison of their replication efficiency in chick embryo was performed. The effect of temperature on viral replication in Madin-Darby canine kidney (MDCK) cells was determined by TCID 50 at 33 °C, 37 °C and 39 °C for 5 consecutive days. Results: S-OIV replicated in cell lines derived from different tissues or organs and host species with comparable viral load to sH1N1. Among 13 human cell lines tested, Caco-2 has the highest viral load for S-OIV. S-OIV showed a low viral load with no CPE or antigen expression in pig kidney cell PK-15, H5N1 demonstrated the most diverse cell tropism by CPE and antigen expression, and the highest viral replication efficiency in both cell lines and allantoic fluid. All three viruses demonstrated best growth at 37 °C in MDCK cells. Conclusion: Cell line growth characteristics of S-OIV, sH1N1 and H5N1 appear to correlate clinically and pathologically with involved anatomical sites and severity. Low replication of S-OIV in PK-15 suggests that this virus is more adapted to human than swine. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.subjectCell tropismen_HK
dc.subjectH5N1en_HK
dc.subjectInfluenza H1N1en_HK
dc.subjectSwine-originen_HK
dc.subjectViral loaden_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBirds - Virologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshChick Embryoen_US
dc.subject.meshDogsen_US
dc.subject.meshHumansen_US
dc.subject.meshInfluenza A Virus, H1n1 Subtype - Physiologyen_US
dc.subject.meshInfluenza A Virus, H5n1 Subtype - Physiologyen_US
dc.subject.meshInfluenza In Birds - Virologyen_US
dc.subject.meshInfluenza, Human - Virologyen_US
dc.subject.meshSwine - Virologyen_US
dc.subject.meshSwine Diseases - Virologyen_US
dc.subject.meshTemperatureen_US
dc.subject.meshViral Loaden_US
dc.subject.meshVirus Replicationen_US
dc.titleDifferential susceptibility of different cell lines to swine-origin influenza A H1N1, seasonal human influenza A H1N1, and avian influenza A H5N1 virusesen_HK
dc.typeArticleen_HK
dc.identifier.emailTo, KWK: kelvinto@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY: samsonsy@hkucc.hku.hken_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW: hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailChan, JFW: jfwchan@hku.hken_HK
dc.identifier.emailZheng, BJ: bzheng@hkucc.hku.hken_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityTo, KWK=rp01384en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityChan, JFW=rp01736en_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jcv.2009.09.013en_HK
dc.identifier.pmid19801200en_HK
dc.identifier.scopuseid_2-s2.0-71549137759en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71549137759&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue4en_HK
dc.identifier.spage325en_HK
dc.identifier.epage330en_HK
dc.identifier.isiWOS:000272460900006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, IWS=24464179500en_HK
dc.identifier.scopusauthoridChan, KH=35338760600en_HK
dc.identifier.scopusauthoridTo, KWK=14323807300en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridChan, JFW=24278817900en_HK
dc.identifier.scopusauthoridCheng, VCC=23670479400en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike5914365-

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