File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Detection of human novel influenza A (H1N1) viruses using multi-fluorescent real-time RT-PCR
  • Basic View
  • Metadata View
  • XML View
TitleDetection of human novel influenza A (H1N1) viruses using multi-fluorescent real-time RT-PCR
 
AuthorsDong, H2
Zhang, Y1
Xiong, H2
Yan, A2
Ding, G5
Chen, Y2 4
Xie, L2
Chen, J2
Zhang, G5
Hao, P5
Cong, L1
Lu, Y1
Che, X6
Wang, X7
Li, Y5
Yuen, KY3
Zhao, G8 4 2
Jin, W2 4
 
Issue Date2010
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusres
 
CitationVirus Research, 2010, v. 147 n. 1, p. 85-90 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virusres.2009.10.011
 
AbstractThe novel influenza A (H1N1) virus is now rapidly spreading across the world. Early detection is one of the most effective measures to prevent further transmission of the virus. 4 sets of proprietary primers and probes designed for detection of influenza A viruses (InfA), human and swine H1N1 viruses (SH1), the novel H1N1 viruses (NH1) and RNaseP gene (RP) respectively were pooled together in a single tube for a multi-fluorescent real-time RT-PCR assay. The detection limit was found to be one order more sensitive than that employing the WHO recommended protocol. The NH1 probe was negative for all control samples including human seasonal H1N1 virus, other subtypes of human influenza A viruses (H3, H5, H9), human influenza B virus and nasopharyngeal swabs from patients with noninfluenza respiratory diseases, indicating its high specificity, capable of discriminating the novel influenza A virus from the previously identified H1N1 viruses. For confirmation, the PCR amplified fragment of the hemagglutinin gene was sequenced which could provide enough information to identify the novel H1N1 virus as a distinct cluster among all viruses of subtype H1 through average distance clustering analysis. Although these assays should be useful in the current outbreak for rapid detection and discrimination of the novel H1N1 from swine H1N1 and other human seasonal H1N1 viruses, further design improvement is suggested to match possible sequence variations in the detected region along with the course of the epidemic. © 2009 Elsevier B.V. All rights reserved.
 
ISSN0168-1702
2012 Impact Factor: 2.745
2012 SCImago Journal Rankings: 0.999
 
DOIhttp://dx.doi.org/10.1016/j.virusres.2009.10.011
 
ISI Accession Number IDWOS:000276560300012
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorDong, H
 
dc.contributor.authorZhang, Y
 
dc.contributor.authorXiong, H
 
dc.contributor.authorYan, A
 
dc.contributor.authorDing, G
 
dc.contributor.authorChen, Y
 
dc.contributor.authorXie, L
 
dc.contributor.authorChen, J
 
dc.contributor.authorZhang, G
 
dc.contributor.authorHao, P
 
dc.contributor.authorCong, L
 
dc.contributor.authorLu, Y
 
dc.contributor.authorChe, X
 
dc.contributor.authorWang, X
 
dc.contributor.authorLi, Y
 
dc.contributor.authorYuen, KY
 
dc.contributor.authorZhao, G
 
dc.contributor.authorJin, W
 
dc.date.accessioned2012-08-08T08:51:20Z
 
dc.date.available2012-08-08T08:51:20Z
 
dc.date.issued2010
 
dc.description.abstractThe novel influenza A (H1N1) virus is now rapidly spreading across the world. Early detection is one of the most effective measures to prevent further transmission of the virus. 4 sets of proprietary primers and probes designed for detection of influenza A viruses (InfA), human and swine H1N1 viruses (SH1), the novel H1N1 viruses (NH1) and RNaseP gene (RP) respectively were pooled together in a single tube for a multi-fluorescent real-time RT-PCR assay. The detection limit was found to be one order more sensitive than that employing the WHO recommended protocol. The NH1 probe was negative for all control samples including human seasonal H1N1 virus, other subtypes of human influenza A viruses (H3, H5, H9), human influenza B virus and nasopharyngeal swabs from patients with noninfluenza respiratory diseases, indicating its high specificity, capable of discriminating the novel influenza A virus from the previously identified H1N1 viruses. For confirmation, the PCR amplified fragment of the hemagglutinin gene was sequenced which could provide enough information to identify the novel H1N1 virus as a distinct cluster among all viruses of subtype H1 through average distance clustering analysis. Although these assays should be useful in the current outbreak for rapid detection and discrimination of the novel H1N1 from swine H1N1 and other human seasonal H1N1 viruses, further design improvement is suggested to match possible sequence variations in the detected region along with the course of the epidemic. © 2009 Elsevier B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationVirus Research, 2010, v. 147 n. 1, p. 85-90 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virusres.2009.10.011
 
dc.identifier.citeulike6199132
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.virusres.2009.10.011
 
dc.identifier.epage90
 
dc.identifier.isiWOS:000276560300012
 
dc.identifier.issn0168-1702
2012 Impact Factor: 2.745
2012 SCImago Journal Rankings: 0.999
 
dc.identifier.issue1
 
dc.identifier.pmid19883704
 
dc.identifier.scopuseid_2-s2.0-71349088322
 
dc.identifier.spage85
 
dc.identifier.urihttp://hdl.handle.net/10722/157567
 
dc.identifier.volume147
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusres
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofVirus Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCluster Analysis
 
dc.subject.meshDna Primers - Genetics
 
dc.subject.meshGenotype
 
dc.subject.meshHemagglutinins, Viral - Genetics
 
dc.subject.meshHumans
 
dc.subject.meshInfluenza A Virus, H1n1 Subtype - Classification - Genetics - Isolation & Purification
 
dc.subject.meshInfluenza, Human - Diagnosis - Virology
 
dc.subject.meshRna, Viral - Genetics
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Methods
 
dc.subject.meshSensitivity And Specificity
 
dc.subject.meshSequence Analysis, Dna
 
dc.subject.meshSequence Homology
 
dc.titleDetection of human novel influenza A (H1N1) viruses using multi-fluorescent real-time RT-PCR
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Dong, H</contributor.author>
<contributor.author>Zhang, Y</contributor.author>
<contributor.author>Xiong, H</contributor.author>
<contributor.author>Yan, A</contributor.author>
<contributor.author>Ding, G</contributor.author>
<contributor.author>Chen, Y</contributor.author>
<contributor.author>Xie, L</contributor.author>
<contributor.author>Chen, J</contributor.author>
<contributor.author>Zhang, G</contributor.author>
<contributor.author>Hao, P</contributor.author>
<contributor.author>Cong, L</contributor.author>
<contributor.author>Lu, Y</contributor.author>
<contributor.author>Che, X</contributor.author>
<contributor.author>Wang, X</contributor.author>
<contributor.author>Li, Y</contributor.author>
<contributor.author>Yuen, KY</contributor.author>
<contributor.author>Zhao, G</contributor.author>
<contributor.author>Jin, W</contributor.author>
<date.accessioned>2012-08-08T08:51:20Z</date.accessioned>
<date.available>2012-08-08T08:51:20Z</date.available>
<date.issued>2010</date.issued>
<identifier.citation>Virus Research, 2010, v. 147 n. 1, p. 85-90</identifier.citation>
<identifier.issn>0168-1702</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/157567</identifier.uri>
<description.abstract>The novel influenza A (H1N1) virus is now rapidly spreading across the world. Early detection is one of the most effective measures to prevent further transmission of the virus. 4 sets of proprietary primers and probes designed for detection of influenza A viruses (InfA), human and swine H1N1 viruses (SH1), the novel H1N1 viruses (NH1) and RNaseP gene (RP) respectively were pooled together in a single tube for a multi-fluorescent real-time RT-PCR assay. The detection limit was found to be one order more sensitive than that employing the WHO recommended protocol. The NH1 probe was negative for all control samples including human seasonal H1N1 virus, other subtypes of human influenza A viruses (H3, H5, H9), human influenza B virus and nasopharyngeal swabs from patients with noninfluenza respiratory diseases, indicating its high specificity, capable of discriminating the novel influenza A virus from the previously identified H1N1 viruses. For confirmation, the PCR amplified fragment of the hemagglutinin gene was sequenced which could provide enough information to identify the novel H1N1 virus as a distinct cluster among all viruses of subtype H1 through average distance clustering analysis. Although these assays should be useful in the current outbreak for rapid detection and discrimination of the novel H1N1 from swine H1N1 and other human seasonal H1N1 viruses, further design improvement is suggested to match possible sequence variations in the detected region along with the course of the epidemic. &#169; 2009 Elsevier B.V. All rights reserved.</description.abstract>
<language>eng</language>
<publisher>Elsevier BV. The Journal&apos;s web site is located at http://www.elsevier.com/locate/virusres</publisher>
<relation.ispartof>Virus Research</relation.ispartof>
<subject.mesh>Cluster Analysis</subject.mesh>
<subject.mesh>Dna Primers - Genetics</subject.mesh>
<subject.mesh>Genotype</subject.mesh>
<subject.mesh>Hemagglutinins, Viral - Genetics</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Influenza A Virus, H1n1 Subtype - Classification - Genetics - Isolation &amp; Purification</subject.mesh>
<subject.mesh>Influenza, Human - Diagnosis - Virology</subject.mesh>
<subject.mesh>Rna, Viral - Genetics</subject.mesh>
<subject.mesh>Reverse Transcriptase Polymerase Chain Reaction - Methods</subject.mesh>
<subject.mesh>Sensitivity And Specificity</subject.mesh>
<subject.mesh>Sequence Analysis, Dna</subject.mesh>
<subject.mesh>Sequence Homology</subject.mesh>
<title>Detection of human novel influenza A (H1N1) viruses using multi-fluorescent real-time RT-PCR</title>
<type>Article</type>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1016/j.virusres.2009.10.011</identifier.doi>
<identifier.pmid>19883704</identifier.pmid>
<identifier.scopus>eid_2-s2.0-71349088322</identifier.scopus>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-71349088322&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>147</identifier.volume>
<identifier.issue>1</identifier.issue>
<identifier.spage>85</identifier.spage>
<identifier.epage>90</identifier.epage>
<identifier.isi>WOS:000276560300012</identifier.isi>
<publisher.place>Netherlands</publisher.place>
<identifier.citeulike>6199132</identifier.citeulike>
</item>
Author Affiliations
  1. Zhejiang Provincial Center for Disease Control and Prevention
  2. Chinese National Human Genome Center at Shanghai
  3. The University of Hong Kong
  4. National Engineering Center for Biochip at Shanghai
  5. Shanghai Institute for Biological Sciences Chinese Academy of Sciences
  6. Zhujiang Hospital
  7. South China University of Technology
  8. Chinese University of Hong Kong