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Article: Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: Evidence for the processing of a viral-cellular double-stranded RNA hybrid

TitlePyrosequencing of small non-coding RNAs in HIV-1 infected cells: Evidence for the processing of a viral-cellular double-stranded RNA hybrid
Authors
Issue Date2009
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2009, v. 37 n. 19, p. 6575-6586 How to Cite?
AbstractSmall non-coding RNAs of 18-25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3′ end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1. © The Author(s) 2009. Published by Oxford University Press.
Persistent Identifierhttp://hdl.handle.net/10722/157563
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID
Funding AgencyGrant Number
NIAID
NCI
IATAP
Funding Information:

NIAID; NCI; IATAP program from the office of the director, National Institutes of Health. Funding for open access charge: National Institutes of Health intramural funds.

References

 

DC FieldValueLanguage
dc.contributor.authorYeung, MLen_US
dc.contributor.authorBennasser, Yen_US
dc.contributor.authorWatashi, Ken_US
dc.contributor.authorLe, SYen_US
dc.contributor.authorHouzet, Len_US
dc.contributor.authorJeang, KTen_US
dc.date.accessioned2012-08-08T08:51:16Z-
dc.date.available2012-08-08T08:51:16Z-
dc.date.issued2009en_US
dc.identifier.citationNucleic Acids Research, 2009, v. 37 n. 19, p. 6575-6586en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/157563-
dc.description.abstractSmall non-coding RNAs of 18-25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3′ end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1. © The Author(s) 2009. Published by Oxford University Press.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshCell Lineen_US
dc.subject.meshHiv-1 - Genetics - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshRna, Double-Stranded - Metabolismen_US
dc.subject.meshRna, Transfer, Lys - Metabolismen_US
dc.subject.meshRna, Untranslated - Chemistry - Metabolismen_US
dc.subject.meshRna, Viral - Metabolismen_US
dc.subject.meshSequence Analysis, Rnaen_US
dc.subject.meshT-Lymphocytes - Virologyen_US
dc.subject.meshVirus Replicationen_US
dc.titlePyrosequencing of small non-coding RNAs in HIV-1 infected cells: Evidence for the processing of a viral-cellular double-stranded RNA hybriden_US
dc.typeArticleen_US
dc.identifier.emailYeung, ML:pmlyeung@hku.hken_US
dc.identifier.authorityYeung, ML=rp01402en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/gkp707en_US
dc.identifier.pmid19729508-
dc.identifier.scopuseid_2-s2.0-70449604918en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70449604918&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume37en_US
dc.identifier.issue19en_US
dc.identifier.spage6575en_US
dc.identifier.epage6586en_US
dc.identifier.isiWOS:000271389900024-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYeung, ML=8350940900en_US
dc.identifier.scopusauthoridBennasser, Y=8335747500en_US
dc.identifier.scopusauthoridWatashi, K=6603024689en_US
dc.identifier.scopusauthoridLe, SY=7006184376en_US
dc.identifier.scopusauthoridHouzet, L=7801516884en_US
dc.identifier.scopusauthoridJeang, KT=7004824803en_US
dc.identifier.citeulike8269590-

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