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- Publisher Website: 10.1007/978-1-60327-302-2_11
- Scopus: eid_2-s2.0-70350075758
- PMID: 19554293
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Article: Sequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies.
Title | Sequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies. |
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Authors | |
Issue Date | 2009 |
Citation | Methods In Molecular Biology (Clifton, N.J.), 2009, v. 562, p. 143-154 How to Cite? |
Abstract | Many phage display techniques drive selection toward the isolation of highly specific antibodies. However, the identification of monoclonal antibodies that are cross-reactive has implications for the development of diagnostics, therapeutics, and vaccines against pathogens or cancer cells that are able to rapidly generate variants and escape mutants. To identify human monoclonal antibodies with high activity against HIV and broad-spectrum activity, we developed a technique termed sequential antigen panning. This methodology could be used to isolated recombinant antibodies against any antigen that shares epitopes with other antigens. |
Persistent Identifier | http://hdl.handle.net/10722/157559 |
ISBN | |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, MY | en_US |
dc.contributor.author | Dimitrov, DS | en_US |
dc.date.accessioned | 2012-08-08T08:51:14Z | - |
dc.date.available | 2012-08-08T08:51:14Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Methods In Molecular Biology (Clifton, N.J.), 2009, v. 562, p. 143-154 | en_US |
dc.identifier.isbn | 9781603273015 | - |
dc.identifier.issn | 1064-3745 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/157559 | - |
dc.description.abstract | Many phage display techniques drive selection toward the isolation of highly specific antibodies. However, the identification of monoclonal antibodies that are cross-reactive has implications for the development of diagnostics, therapeutics, and vaccines against pathogens or cancer cells that are able to rapidly generate variants and escape mutants. To identify human monoclonal antibodies with high activity against HIV and broad-spectrum activity, we developed a technique termed sequential antigen panning. This methodology could be used to isolated recombinant antibodies against any antigen that shares epitopes with other antigens. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Methods in molecular biology (Clifton, N.J.) | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Genetics - Immunology - Isolation & Purification | en_US |
dc.subject.mesh | Cross Reactions - Immunology | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay - Methods | en_US |
dc.subject.mesh | Hiv Antibodies - Immunology | en_US |
dc.subject.mesh | Hiv Envelope Protein Gp120 - Immunology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunoglobulin Fab Fragments - Immunology | en_US |
dc.subject.mesh | Neutralization Tests | en_US |
dc.subject.mesh | Peptide Library | en_US |
dc.subject.mesh | Env Gene Products, Human Immunodeficiency Virus - Immunology | en_US |
dc.title | Sequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Zhang, MY:zhangmy@hku.hk | en_US |
dc.identifier.authority | Zhang, MY=rp01409 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/978-1-60327-302-2_11 | en_US |
dc.identifier.pmid | 19554293 | - |
dc.identifier.scopus | eid_2-s2.0-70350075758 | en_US |
dc.identifier.hkuros | 170457 | - |
dc.identifier.volume | 562 | en_US |
dc.identifier.spage | 143 | en_US |
dc.identifier.epage | 154 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Zhang, MY=35316639300 | en_US |
dc.identifier.scopusauthorid | Dimitrov, DS=7202564539 | en_US |
dc.identifier.issnl | 1064-3745 | - |