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Article: Sequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies.

TitleSequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies.
Authors
Issue Date2009
Citation
Methods In Molecular Biology (Clifton, N.J.), 2009, v. 562, p. 143-154 How to Cite?
AbstractMany phage display techniques drive selection toward the isolation of highly specific antibodies. However, the identification of monoclonal antibodies that are cross-reactive has implications for the development of diagnostics, therapeutics, and vaccines against pathogens or cancer cells that are able to rapidly generate variants and escape mutants. To identify human monoclonal antibodies with high activity against HIV and broad-spectrum activity, we developed a technique termed sequential antigen panning. This methodology could be used to isolated recombinant antibodies against any antigen that shares epitopes with other antigens.
Persistent Identifierhttp://hdl.handle.net/10722/157559
ISBN
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorZhang, MYen_US
dc.contributor.authorDimitrov, DSen_US
dc.date.accessioned2012-08-08T08:51:14Z-
dc.date.available2012-08-08T08:51:14Z-
dc.date.issued2009en_US
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2009, v. 562, p. 143-154en_US
dc.identifier.isbn9781603273015-
dc.identifier.issn1064-3745en_US
dc.identifier.urihttp://hdl.handle.net/10722/157559-
dc.description.abstractMany phage display techniques drive selection toward the isolation of highly specific antibodies. However, the identification of monoclonal antibodies that are cross-reactive has implications for the development of diagnostics, therapeutics, and vaccines against pathogens or cancer cells that are able to rapidly generate variants and escape mutants. To identify human monoclonal antibodies with high activity against HIV and broad-spectrum activity, we developed a technique termed sequential antigen panning. This methodology could be used to isolated recombinant antibodies against any antigen that shares epitopes with other antigens.en_US
dc.languageengen_US
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_US
dc.subject.meshAntibodies, Monoclonal - Genetics - Immunology - Isolation & Purificationen_US
dc.subject.meshCross Reactions - Immunologyen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methodsen_US
dc.subject.meshHiv Antibodies - Immunologyen_US
dc.subject.meshHiv Envelope Protein Gp120 - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Fab Fragments - Immunologyen_US
dc.subject.meshNeutralization Testsen_US
dc.subject.meshPeptide Libraryen_US
dc.subject.meshEnv Gene Products, Human Immunodeficiency Virus - Immunologyen_US
dc.titleSequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies.en_US
dc.typeArticleen_US
dc.identifier.emailZhang, MY:zhangmy@hku.hken_US
dc.identifier.authorityZhang, MY=rp01409en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/978-1-60327-302-2_11en_US
dc.identifier.pmid19554293-
dc.identifier.scopuseid_2-s2.0-70350075758en_US
dc.identifier.hkuros170457-
dc.identifier.volume562en_US
dc.identifier.spage143en_US
dc.identifier.epage154en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhang, MY=35316639300en_US
dc.identifier.scopusauthoridDimitrov, DS=7202564539en_US

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