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Article: Analysis of H5N1 avian influenza infections from wild bird surveillance in Hong Kong from January 2006 to October 2007
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TitleAnalysis of H5N1 avian influenza infections from wild bird surveillance in Hong Kong from January 2006 to October 2007
 
AuthorsEllis, TM3 1
Dyrting, KC3
Wong, CW3
Chadwick, B3
Chan, C3
Chiang, M3
Li, C3
Li, P3
Smith, GJD2
Guan, Y2
Malik Peiris, JS2
 
Issue Date2009
 
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03079457.asp
 
CitationAvian Pathology, 2009, v. 38 n. 2, p. 107-119 [How to Cite?]
DOI: http://dx.doi.org/10.1080/03079450902751855
 
AbstractIntensive surveillance of dead wild birds for H5N1 avian influenza infection is conducted in Hong Kong. Between January 2006 and October 2007 pooled cloacal and tracheal swabs from 17692 dead wild birds (from 16 different orders including 82 genera) were tested and 33 H5N1 highly pathogenic avian influenza viruses were isolated. No H5N1 infection has occurred in poultry farms since January 2003, or in live poultry markets in Hong Kong since November 2003 until a recent detection of H5N1 virus by surveillance of live poultry markets in June 2008. The gross and histopathology in the various H5N1-infected avian species is described, along with the performance of the virus isolation and polymerase chain reaction (PCR) tests used. This evaluation also included determination of virus titres and detection limits for the H5 haemagglutinin gene (H5)and matrix gene real-time reverse-transcription PCR tests in cloacal and tracheal swabs from 12 wild birds. The viruses isolated belonged to Clades 2.3.2 and 2.3.4, and within Clade 2.3.4 some clustering was evident based on H5 haemagglutinin gene haemagglutinating sequencing. There were no differences in the pathology findings between these subgroupings and the various diagnostic tests gave similar results for these viruses, except for a loss in sensitivity of the H5 real-time reverse-transcription PCR for several viruses in one cluster from birds submitted in February 2007. The use of multiple test methods was important in maintaining the diagnostic sensitivity for detecting avian influenza viruses with high genetic variability. © 2009 Houghton Trust Ltd.
 
ISSN0307-9457
2012 Impact Factor: 1.729
2012 SCImago Journal Rankings: 0.834
 
DOIhttp://dx.doi.org/10.1080/03079450902751855
 
ISI Accession Number IDWOS:000264529100003
Funding AgencyGrant Number
Avian Virology, Histology and Molecular Biology laboratories at Tai Lung Veterinary Laboratory
Centre for Emerging Infectious Diseases at the Department of Microbiology, The University of Hong Kong
Dr Thomas Sit, Assistant Director Inspection and Quarantine, AFCD
Research Grants CouncilHKU 7512/06M
Hong Kong SAR Government
Area Excellence Scheme of the University Grants CommitteeAoE/M-12/6
National Institutes of Health (National Institute of Allergy and Infectious Diseases [NIAID])HHSN266200700005C
NIAIDHHSN266200700005C
Funding Information:

The authors acknowledge the excellent technical support provided by the staff of the Avian Virology, Histology and Molecular Biology laboratories at Tai Lung Veterinary Laboratory and the staff of the Centre for Emerging Infectious Diseases at the Department of Microbiology, The University of Hong Kong. They thank all staff from the Agriculture Fisheries and Conservation Department and the Leisure and Cultural Services Department of the Hong Kong SAR Government for collection and submission of dead bird carcasses, and particularly thank Mr W. H. Lee, Ornithologist from the Conservation Branch, AFCD for assistance with identification of wild bird species. The authors thank Dr David Suarez and Dr Erica Spackman of South East Poultry Research Laboratory, USDA, Athens, Georgia, USA and Dr Hans Heine of the Australian Animal Health Laboratory, CSIRO, Geelong, Australia for the RRT-PCR primer and probe sequences; Dr Peter Hooper of AAHL-CSIRO for the influenza A nucleoprotein monoclonal antibody and Dr Nancy Cox of Centres for Disease Control, Atlanta, Georgia, USA for the H5-HA-specific monoclonal antibodies. The support of Dr Thomas Sit, Assistant Director Inspection and Quarantine, AFCD for this work is gratefully acknowledged. This study was in part supported by the Research Grants Council (HKU 7512/06M) of the Hong Kong SAR Government, the Area Excellence Scheme of the University Grants Committee (grant AoE/M-12/6) and the National Institutes of Health (National Institute of Allergy and Infectious Diseases [NIAID] contract HHSN266200700005C). G.J.D.S. is supported by a career development award under NIAID contract HHSN266200700005C. The views expressed in this paper are those of the authors only, and may not represent the opinion of the Agriculture, Fisheries and Conservation Department.

 
ReferencesReferences in Scopus
 
GrantsThe role of migratory birds in the transmission of H5N1 Influenza virus in eastern Asia
 
DC FieldValue
dc.contributor.authorEllis, TM
 
dc.contributor.authorDyrting, KC
 
dc.contributor.authorWong, CW
 
dc.contributor.authorChadwick, B
 
dc.contributor.authorChan, C
 
dc.contributor.authorChiang, M
 
dc.contributor.authorLi, C
 
dc.contributor.authorLi, P
 
dc.contributor.authorSmith, GJD
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorMalik Peiris, JS
 
dc.date.accessioned2012-08-08T08:51:08Z
 
dc.date.available2012-08-08T08:51:08Z
 
dc.date.issued2009
 
dc.description.abstractIntensive surveillance of dead wild birds for H5N1 avian influenza infection is conducted in Hong Kong. Between January 2006 and October 2007 pooled cloacal and tracheal swabs from 17692 dead wild birds (from 16 different orders including 82 genera) were tested and 33 H5N1 highly pathogenic avian influenza viruses were isolated. No H5N1 infection has occurred in poultry farms since January 2003, or in live poultry markets in Hong Kong since November 2003 until a recent detection of H5N1 virus by surveillance of live poultry markets in June 2008. The gross and histopathology in the various H5N1-infected avian species is described, along with the performance of the virus isolation and polymerase chain reaction (PCR) tests used. This evaluation also included determination of virus titres and detection limits for the H5 haemagglutinin gene (H5)and matrix gene real-time reverse-transcription PCR tests in cloacal and tracheal swabs from 12 wild birds. The viruses isolated belonged to Clades 2.3.2 and 2.3.4, and within Clade 2.3.4 some clustering was evident based on H5 haemagglutinin gene haemagglutinating sequencing. There were no differences in the pathology findings between these subgroupings and the various diagnostic tests gave similar results for these viruses, except for a loss in sensitivity of the H5 real-time reverse-transcription PCR for several viruses in one cluster from birds submitted in February 2007. The use of multiple test methods was important in maintaining the diagnostic sensitivity for detecting avian influenza viruses with high genetic variability. © 2009 Houghton Trust Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationAvian Pathology, 2009, v. 38 n. 2, p. 107-119 [How to Cite?]
DOI: http://dx.doi.org/10.1080/03079450902751855
 
dc.identifier.citeulike4233618
 
dc.identifier.doihttp://dx.doi.org/10.1080/03079450902751855
 
dc.identifier.epage119
 
dc.identifier.isiWOS:000264529100003
Funding AgencyGrant Number
Avian Virology, Histology and Molecular Biology laboratories at Tai Lung Veterinary Laboratory
Centre for Emerging Infectious Diseases at the Department of Microbiology, The University of Hong Kong
Dr Thomas Sit, Assistant Director Inspection and Quarantine, AFCD
Research Grants CouncilHKU 7512/06M
Hong Kong SAR Government
Area Excellence Scheme of the University Grants CommitteeAoE/M-12/6
National Institutes of Health (National Institute of Allergy and Infectious Diseases [NIAID])HHSN266200700005C
NIAIDHHSN266200700005C
Funding Information:

The authors acknowledge the excellent technical support provided by the staff of the Avian Virology, Histology and Molecular Biology laboratories at Tai Lung Veterinary Laboratory and the staff of the Centre for Emerging Infectious Diseases at the Department of Microbiology, The University of Hong Kong. They thank all staff from the Agriculture Fisheries and Conservation Department and the Leisure and Cultural Services Department of the Hong Kong SAR Government for collection and submission of dead bird carcasses, and particularly thank Mr W. H. Lee, Ornithologist from the Conservation Branch, AFCD for assistance with identification of wild bird species. The authors thank Dr David Suarez and Dr Erica Spackman of South East Poultry Research Laboratory, USDA, Athens, Georgia, USA and Dr Hans Heine of the Australian Animal Health Laboratory, CSIRO, Geelong, Australia for the RRT-PCR primer and probe sequences; Dr Peter Hooper of AAHL-CSIRO for the influenza A nucleoprotein monoclonal antibody and Dr Nancy Cox of Centres for Disease Control, Atlanta, Georgia, USA for the H5-HA-specific monoclonal antibodies. The support of Dr Thomas Sit, Assistant Director Inspection and Quarantine, AFCD for this work is gratefully acknowledged. This study was in part supported by the Research Grants Council (HKU 7512/06M) of the Hong Kong SAR Government, the Area Excellence Scheme of the University Grants Committee (grant AoE/M-12/6) and the National Institutes of Health (National Institute of Allergy and Infectious Diseases [NIAID] contract HHSN266200700005C). G.J.D.S. is supported by a career development award under NIAID contract HHSN266200700005C. The views expressed in this paper are those of the authors only, and may not represent the opinion of the Agriculture, Fisheries and Conservation Department.

 
dc.identifier.issn0307-9457
2012 Impact Factor: 1.729
2012 SCImago Journal Rankings: 0.834
 
dc.identifier.issue2
 
dc.identifier.scopuseid_2-s2.0-65649094077
 
dc.identifier.spage107
 
dc.identifier.urihttp://hdl.handle.net/10722/157546
 
dc.identifier.volume38
 
dc.languageeng
 
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03079457.asp
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofAvian Pathology
 
dc.relation.projectThe role of migratory birds in the transmission of H5N1 Influenza virus in eastern Asia
 
dc.relation.referencesReferences in Scopus
 
dc.titleAnalysis of H5N1 avian influenza infections from wild bird surveillance in Hong Kong from January 2006 to October 2007
 
dc.typeArticle
 
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<contributor.author>Chiang, M</contributor.author>
<contributor.author>Li, C</contributor.author>
<contributor.author>Li, P</contributor.author>
<contributor.author>Smith, GJD</contributor.author>
<contributor.author>Guan, Y</contributor.author>
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Author Affiliations
  1. Murdoch University
  2. The University of Hong Kong
  3. Tai Lung Veterinary Laboratory