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- Scopus: eid_2-s2.0-64949096179
- PMID: 19252830
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Article: Competitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41.
Title | Competitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41. |
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Authors | |
Issue Date | 2009 |
Citation | Methods In Molecular Biology (Clifton, N.J.), 2009, v. 525, p. 175-186, xv How to Cite? |
Abstract | HIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against gp140 (covalently linked gp120 and the extracellular portion of gp41) has resulted mostly in selection of anti-gp120 antibodies. Native gp41 in the absence of gp120 is unstable. The use of gp41 fragments as antigens has resulted in selection of antibodies with only relatively modest neutralizing activity. To enhance selection of antibodies specific for gp41 in the context of the whole Env we developed a methodology termed competitive antigen panning (CAP). Using CAP, we identified a panel of gp41-specific human monoclonal antibodies from an HIV-1 immune library derived from long-term nonprogressors. These antibodies recognize conformational epitopes in gp41 and exhibited, to various extents, neutralization activity in assays based on spreading infection in peripheral blood mononuclear cells. The CAP methodology is generally applicable for selection of antibodies specific for any epitope that is not a dominant epitope in the antigen. It is superior to a traditional pre-depletion method in avoiding potential loss of target-specific antibodies. |
Persistent Identifier | http://hdl.handle.net/10722/157544 |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, MY | en_US |
dc.contributor.author | Dimitrov, DS | en_US |
dc.date.accessioned | 2012-08-08T08:51:07Z | - |
dc.date.available | 2012-08-08T08:51:07Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Methods In Molecular Biology (Clifton, N.J.), 2009, v. 525, p. 175-186, xv | en_US |
dc.identifier.issn | 1064-3745 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/157544 | - |
dc.description.abstract | HIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against gp140 (covalently linked gp120 and the extracellular portion of gp41) has resulted mostly in selection of anti-gp120 antibodies. Native gp41 in the absence of gp120 is unstable. The use of gp41 fragments as antigens has resulted in selection of antibodies with only relatively modest neutralizing activity. To enhance selection of antibodies specific for gp41 in the context of the whole Env we developed a methodology termed competitive antigen panning (CAP). Using CAP, we identified a panel of gp41-specific human monoclonal antibodies from an HIV-1 immune library derived from long-term nonprogressors. These antibodies recognize conformational epitopes in gp41 and exhibited, to various extents, neutralization activity in assays based on spreading infection in peripheral blood mononuclear cells. The CAP methodology is generally applicable for selection of antibodies specific for any epitope that is not a dominant epitope in the antigen. It is superior to a traditional pre-depletion method in avoiding potential loss of target-specific antibodies. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Methods in molecular biology (Clifton, N.J.) | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Immunology | en_US |
dc.subject.mesh | Antibodies, Viral - Immunology | en_US |
dc.subject.mesh | Antibody Specificity - Immunology | en_US |
dc.subject.mesh | Antigens, Viral - Immunology | en_US |
dc.subject.mesh | Biotinylation | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | en_US |
dc.subject.mesh | Hiv Envelope Protein Gp41 - Immunology | en_US |
dc.subject.mesh | Hiv-1 - Immunology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunoglobulin Fab Fragments - Immunology - Isolation & Purification | en_US |
dc.subject.mesh | Molecular Biology - Methods | en_US |
dc.subject.mesh | Neutralization Tests | en_US |
dc.subject.mesh | Peptide Library | en_US |
dc.subject.mesh | Protein Binding | en_US |
dc.subject.mesh | Recombinant Proteins - Immunology | en_US |
dc.subject.mesh | Solubility | en_US |
dc.title | Competitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Zhang, MY:zhangmy@hku.hk | en_US |
dc.identifier.authority | Zhang, MY=rp01409 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 19252830 | - |
dc.identifier.scopus | eid_2-s2.0-64949096179 | en_US |
dc.identifier.volume | 525 | en_US |
dc.identifier.spage | 175 | en_US |
dc.identifier.epage | 186, xv | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Zhang, MY=35316639300 | en_US |
dc.identifier.scopusauthorid | Dimitrov, DS=7202564539 | en_US |
dc.identifier.issnl | 1064-3745 | - |