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Article: Competitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41.

TitleCompetitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41.
Authors
Issue Date2009
Citation
Methods In Molecular Biology (Clifton, N.J.), 2009, v. 525, p. 175-186, xv How to Cite?
AbstractHIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against gp140 (covalently linked gp120 and the extracellular portion of gp41) has resulted mostly in selection of anti-gp120 antibodies. Native gp41 in the absence of gp120 is unstable. The use of gp41 fragments as antigens has resulted in selection of antibodies with only relatively modest neutralizing activity. To enhance selection of antibodies specific for gp41 in the context of the whole Env we developed a methodology termed competitive antigen panning (CAP). Using CAP, we identified a panel of gp41-specific human monoclonal antibodies from an HIV-1 immune library derived from long-term nonprogressors. These antibodies recognize conformational epitopes in gp41 and exhibited, to various extents, neutralization activity in assays based on spreading infection in peripheral blood mononuclear cells. The CAP methodology is generally applicable for selection of antibodies specific for any epitope that is not a dominant epitope in the antigen. It is superior to a traditional pre-depletion method in avoiding potential loss of target-specific antibodies.
Persistent Identifierhttp://hdl.handle.net/10722/157544
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorZhang, MYen_US
dc.contributor.authorDimitrov, DSen_US
dc.date.accessioned2012-08-08T08:51:07Z-
dc.date.available2012-08-08T08:51:07Z-
dc.date.issued2009en_US
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2009, v. 525, p. 175-186, xven_US
dc.identifier.issn1064-3745en_US
dc.identifier.urihttp://hdl.handle.net/10722/157544-
dc.description.abstractHIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against gp140 (covalently linked gp120 and the extracellular portion of gp41) has resulted mostly in selection of anti-gp120 antibodies. Native gp41 in the absence of gp120 is unstable. The use of gp41 fragments as antigens has resulted in selection of antibodies with only relatively modest neutralizing activity. To enhance selection of antibodies specific for gp41 in the context of the whole Env we developed a methodology termed competitive antigen panning (CAP). Using CAP, we identified a panel of gp41-specific human monoclonal antibodies from an HIV-1 immune library derived from long-term nonprogressors. These antibodies recognize conformational epitopes in gp41 and exhibited, to various extents, neutralization activity in assays based on spreading infection in peripheral blood mononuclear cells. The CAP methodology is generally applicable for selection of antibodies specific for any epitope that is not a dominant epitope in the antigen. It is superior to a traditional pre-depletion method in avoiding potential loss of target-specific antibodies.en_US
dc.languageengen_US
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshAntibodies, Viral - Immunologyen_US
dc.subject.meshAntibody Specificity - Immunologyen_US
dc.subject.meshAntigens, Viral - Immunologyen_US
dc.subject.meshBiotinylationen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHiv Envelope Protein Gp41 - Immunologyen_US
dc.subject.meshHiv-1 - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Fab Fragments - Immunology - Isolation & Purificationen_US
dc.subject.meshMolecular Biology - Methodsen_US
dc.subject.meshNeutralization Testsen_US
dc.subject.meshPeptide Libraryen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRecombinant Proteins - Immunologyen_US
dc.subject.meshSolubilityen_US
dc.titleCompetitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41.en_US
dc.typeArticleen_US
dc.identifier.emailZhang, MY:zhangmy@hku.hken_US
dc.identifier.authorityZhang, MY=rp01409en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid19252830-
dc.identifier.scopuseid_2-s2.0-64949096179en_US
dc.identifier.volume525en_US
dc.identifier.spage175en_US
dc.identifier.epage186, xven_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhang, MY=35316639300en_US
dc.identifier.scopusauthoridDimitrov, DS=7202564539en_US

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