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Article: Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination

TitleRecent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination
Authors
Issue Date2008
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2008, v. 375 n. 2, p. 315-320 How to Cite?
AbstractIn AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.
Persistent Identifierhttp://hdl.handle.net/10722/157519
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPolonis, VRen_US
dc.contributor.authorBrown, BKen_US
dc.contributor.authorBorges, ARen_US
dc.contributor.authorZollaPazner, Sen_US
dc.contributor.authorDimitrov, DSen_US
dc.contributor.authorZhang, MYen_US
dc.contributor.authorBarnett, SWen_US
dc.contributor.authorRuprecht, RMen_US
dc.contributor.authorScarlatti, Gen_US
dc.contributor.authorFenyö, EMen_US
dc.contributor.authorMontefiori, DCen_US
dc.contributor.authorMccutchan, FEen_US
dc.contributor.authorMichael, NLen_US
dc.date.accessioned2012-08-08T08:50:51Z-
dc.date.available2012-08-08T08:50:51Z-
dc.date.issued2008en_US
dc.identifier.citationVirology, 2008, v. 375 n. 2, p. 315-320en_US
dc.identifier.issn0042-6822en_US
dc.identifier.urihttp://hdl.handle.net/10722/157519-
dc.description.abstractIn AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_US
dc.relation.ispartofVirologyen_US
dc.subject.meshAids Vaccines - Immunologyen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshHiv Antibodies - Blood - Immunologyen_US
dc.subject.meshHiv Infections - Blood - Diagnosis - Immunologyen_US
dc.subject.meshHiv-1 - Immunologyen_US
dc.subject.meshHela Cells - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes, Mononuclearen_US
dc.subject.meshLuciferases - Genetics - Metabolismen_US
dc.subject.meshNeutralization Tests - Methodsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshVaccinationen_US
dc.titleRecent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccinationen_US
dc.typeArticleen_US
dc.identifier.emailZhang, MY:zhangmy@hku.hken_US
dc.identifier.authorityZhang, MY=rp01409en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.virol.2008.02.007en_US
dc.identifier.pmid18367229-
dc.identifier.scopuseid_2-s2.0-43249120426en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-43249120426&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume375en_US
dc.identifier.issue2en_US
dc.identifier.spage315en_US
dc.identifier.epage320en_US
dc.identifier.isiWOS:000256106200001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPolonis, VR=6603744791en_US
dc.identifier.scopusauthoridBrown, BK=35748343300en_US
dc.identifier.scopusauthoridBorges, AR=23979626400en_US
dc.identifier.scopusauthoridZollaPazner, S=7006077941en_US
dc.identifier.scopusauthoridDimitrov, DS=7202564539en_US
dc.identifier.scopusauthoridZhang, MY=35316639300en_US
dc.identifier.scopusauthoridBarnett, SW=7202725086en_US
dc.identifier.scopusauthoridRuprecht, RM=7005394971en_US
dc.identifier.scopusauthoridScarlatti, G=7003852850en_US
dc.identifier.scopusauthoridFenyö, EM=7006609785en_US
dc.identifier.scopusauthoridMontefiori, DC=7005651585en_US
dc.identifier.scopusauthoridMcCutchan, FE=7004583326en_US
dc.identifier.scopusauthoridMichael, NL=7006822219en_US

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