File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Design, construction, and characterization of a dual-promoter multigenic DNA vaccine directed against an HIV-1 subtype C/B′ recombinant

TitleDesign, construction, and characterization of a dual-promoter multigenic DNA vaccine directed against an HIV-1 subtype C/B′ recombinant
Authors
Issue Date2008
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jaids.com
Citation
Journal of Acquired Immune Deficiency Syndromes, 2008, v. 47 n. 4, p. 403-411 How to Cite?
AbstractAn effective vaccine against HIV-1 is generally considered the best hope for controlling the raging AIDS pandemic. As a part of our AIDS vaccine development effort, we constructed a dual-promoter plasmid capable of high-level expression of 2 independent transgenes. HIV-1 gag, pol, env, nef, and tat from a primary subtype C/B′ CCR5-tropic HIV-1 were "codon" optimized, modified to eliminate known functional activity, and assembled using an overlapping polymerase chain reaction into 2 plasmids: ADVAX-I (containing env and gag) and ADVAX-II (containing pol and nef-tat). These 2 dual-promoter candidate vaccines showed levels of HIV-1 gene expression comparable to those observed with single-gene plasmids in vitro. Importantly, immunization of mice with these vaccine constructs resulted in dose-dependent multigenic CD4 and CD8 T-cell responses equivalent to those provided by vaccination with single-gene plasmids. With input from the US Food and Drug Administration, ADVAX-I and ADVAX-II have since been combined as a single candidate DNA vaccine, ADVAX. A phase 1 clinical trial of this product has been successfully completed, and its use in prime-boost studies is now underway. © 2008 Lippincott Williams & Wilkins, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/157508
ISSN
2015 Impact Factor: 3.806
2015 SCImago Journal Rankings: 2.434

 

DC FieldValueLanguage
dc.contributor.authorHuang, Yen_US
dc.contributor.authorChen, Zen_US
dc.contributor.authorZhang, Wen_US
dc.contributor.authorGurner, Den_US
dc.contributor.authorSong, Yen_US
dc.contributor.authorGardiner, DFen_US
dc.contributor.authorHo, DDen_US
dc.date.accessioned2012-08-08T08:50:41Z-
dc.date.available2012-08-08T08:50:41Z-
dc.date.issued2008en_US
dc.identifier.citationJournal of Acquired Immune Deficiency Syndromes, 2008, v. 47 n. 4, p. 403-411en_US
dc.identifier.issn1525-4135en_US
dc.identifier.urihttp://hdl.handle.net/10722/157508-
dc.description.abstractAn effective vaccine against HIV-1 is generally considered the best hope for controlling the raging AIDS pandemic. As a part of our AIDS vaccine development effort, we constructed a dual-promoter plasmid capable of high-level expression of 2 independent transgenes. HIV-1 gag, pol, env, nef, and tat from a primary subtype C/B′ CCR5-tropic HIV-1 were "codon" optimized, modified to eliminate known functional activity, and assembled using an overlapping polymerase chain reaction into 2 plasmids: ADVAX-I (containing env and gag) and ADVAX-II (containing pol and nef-tat). These 2 dual-promoter candidate vaccines showed levels of HIV-1 gene expression comparable to those observed with single-gene plasmids in vitro. Importantly, immunization of mice with these vaccine constructs resulted in dose-dependent multigenic CD4 and CD8 T-cell responses equivalent to those provided by vaccination with single-gene plasmids. With input from the US Food and Drug Administration, ADVAX-I and ADVAX-II have since been combined as a single candidate DNA vaccine, ADVAX. A phase 1 clinical trial of this product has been successfully completed, and its use in prime-boost studies is now underway. © 2008 Lippincott Williams & Wilkins, Inc.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jaids.comen_US
dc.relation.ispartofJournal of Acquired Immune Deficiency Syndromesen_US
dc.rightsJournal of Acquired Immune Deficiency Syndromes. Copyright © Lippincott Williams & Wilkins.-
dc.subject.meshAids Vaccines - Administration & Dosage - Genetics - Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCd4-Positive T-Lymphocytes - Cytology - Immunologyen_US
dc.subject.meshCd8-Positive T-Lymphocytes - Cytology - Immunologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshGene Products, ENV - Genetics - Immunology - Metabolismen_US
dc.subject.meshGene Products, GAG - Genetics - Immunology - Metabolismen_US
dc.subject.meshGene Products, NEF - Genetics - Immunology - Metabolismen_US
dc.subject.meshGene Products, POL - Genetics - Immunology - Metabolismen_US
dc.subject.meshGene Products, TAT - Genetics - Immunology - Metabolismen_US
dc.subject.meshHIV Antibodies - Blooden_US
dc.subject.meshHIV-1 - Genetics - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunization - Methodsen_US
dc.subject.meshInterferon-Gamma - Secretionen_US
dc.subject.meshMiceen_US
dc.subject.meshPlasmids - Geneticsen_US
dc.subject.meshPromoter Regions, Genetic - Geneticsen_US
dc.subject.meshRetroviridae Proteins - Genetics - Immunology - Metabolismen_US
dc.subject.meshSpleen - Cytology - Immunology - Metabolismen_US
dc.subject.meshTransfectionen_US
dc.subject.meshVaccines, DNA - Administration & Dosage - Genetics - Immunologyen_US
dc.titleDesign, construction, and characterization of a dual-promoter multigenic DNA vaccine directed against an HIV-1 subtype C/B′ recombinanten_US
dc.typeArticleen_US
dc.identifier.emailChen, Z: zchenai@hkucc.hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/QAI.0b013e3181651b9den_US
dc.identifier.pmid18209683-
dc.identifier.scopuseid_2-s2.0-40549120282en_US
dc.identifier.hkuros147737-
dc.identifier.volume47en_US
dc.identifier.issue4en_US
dc.identifier.spage403en_US
dc.identifier.epage411en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHuang, Y=7501575029en_US
dc.identifier.scopusauthoridChen, Z=35271180800en_US
dc.identifier.scopusauthoridZhang, W=7409430773en_US
dc.identifier.scopusauthoridGurner, D=23491754700en_US
dc.identifier.scopusauthoridSong, Y=10141178900en_US
dc.identifier.scopusauthoridGardiner, DF=7101800882en_US
dc.identifier.scopusauthoridHo, DD=7402971998en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats