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Article: Antigenic profile of avian H5N1 viruses in Asia from 2002 to 2007
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TitleAntigenic profile of avian H5N1 viruses in Asia from 2002 to 2007
 
AuthorsWu, WL
Chen, Y1
Wang, P2
Song, W2
Lau, SY2
Rayner, JM2
Smith, GJ2
Webster, RG4
Peiris, JSM2
Lin, T2 1
Xia, N1
Guan, Y3 2
Chen, H3 2
 
Issue Date2008
 
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
CitationJournal of Virology, 2008, v. 82 n. 4, p. 1798-1807 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.02256-07
 
AbstractAntigenic profiles of post-2002 H5N1 viruses representing major genetic clades and various geographic sources were investigated using a panel of 17 monoclonal antibodies raised from five H5N1 strains. Four antigenic groups from seven clades of H5N1 virus were distinguished and characterized based on their cross-reactivity to the monoclonal antibodies in hemagglutination inhibition and cell-based neutralization assays. Genetic polymorphisms associated with the variation of antigenicity of H5N1 strains were identified and further verified in antigenic analysis with recombinant H5N1 viruses carrying specific mutations in the hemagglutinin protein. Modification of some of these genetic variations produced marked improvement to the immunogenicity and cross-reactivity of H5N1 strains in assays utilizing monoclonal antibodies and ferret antisera raised against clade 1 and 2 H5N1 viruses, suggesting that these sites represent antigenically significant amino acids. These results provide a comprehensive antigenic profile for H5N1 virus strains circulating in recent years and will facilitate the recognition of emerging antigenic variants of H5N1 virus and aid in the selection of vaccine strains. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
 
DescriptionOpen Access Journal
 
ISSN0022-538X
2013 Impact Factor: 4.648
 
DOIhttp://dx.doi.org/10.1128/JVI.02256-07
 
PubMed Central IDPMC2258718
 
ISI Accession Number IDWOS:000252909300016
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWu, WL
 
dc.contributor.authorChen, Y
 
dc.contributor.authorWang, P
 
dc.contributor.authorSong, W
 
dc.contributor.authorLau, SY
 
dc.contributor.authorRayner, JM
 
dc.contributor.authorSmith, GJ
 
dc.contributor.authorWebster, RG
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorLin, T
 
dc.contributor.authorXia, N
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorChen, H
 
dc.date.accessioned2012-08-08T08:50:39Z
 
dc.date.available2012-08-08T08:50:39Z
 
dc.date.issued2008
 
dc.description.abstractAntigenic profiles of post-2002 H5N1 viruses representing major genetic clades and various geographic sources were investigated using a panel of 17 monoclonal antibodies raised from five H5N1 strains. Four antigenic groups from seven clades of H5N1 virus were distinguished and characterized based on their cross-reactivity to the monoclonal antibodies in hemagglutination inhibition and cell-based neutralization assays. Genetic polymorphisms associated with the variation of antigenicity of H5N1 strains were identified and further verified in antigenic analysis with recombinant H5N1 viruses carrying specific mutations in the hemagglutinin protein. Modification of some of these genetic variations produced marked improvement to the immunogenicity and cross-reactivity of H5N1 strains in assays utilizing monoclonal antibodies and ferret antisera raised against clade 1 and 2 H5N1 viruses, suggesting that these sites represent antigenically significant amino acids. These results provide a comprehensive antigenic profile for H5N1 virus strains circulating in recent years and will facilitate the recognition of emerging antigenic variants of H5N1 virus and aid in the selection of vaccine strains. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.descriptionOpen Access Journal
 
dc.identifier.citationJournal of Virology, 2008, v. 82 n. 4, p. 1798-1807 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.02256-07
 
dc.identifier.citeulike2320259
 
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.02256-07
 
dc.identifier.eissn1098-5514
 
dc.identifier.epage1807
 
dc.identifier.hkuros146122
 
dc.identifier.isiWOS:000252909300016
 
dc.identifier.issn0022-538X
2013 Impact Factor: 4.648
 
dc.identifier.issue4
 
dc.identifier.pmcidPMC2258718
 
dc.identifier.pmid18077726
 
dc.identifier.scopuseid_2-s2.0-38949120565
 
dc.identifier.spage1798
 
dc.identifier.urihttp://hdl.handle.net/10722/157504
 
dc.identifier.volume82
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Virology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.
 
dc.subject.meshAmino Acid Sequence
 
dc.subject.meshAnimals
 
dc.subject.meshAntibodies, Monoclonal - Immunology
 
dc.subject.meshAntigenic Variation
 
dc.subject.meshAntigens, Viral - Classification - Genetics - Immunology
 
dc.subject.meshAsia - Epidemiology
 
dc.subject.meshBirds
 
dc.subject.meshCross Reactions
 
dc.subject.meshHemagglutinins, Viral - Classification - Genetics - Immunology
 
dc.subject.meshInfluenza A Virus, H5N1 Subtype - Genetics - Immunology
 
dc.subject.meshInfluenza Vaccines - Genetics - Immunology
 
dc.subject.meshInfluenza In Birds - Epidemiology - Virology
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshNeutralization Tests
 
dc.subject.meshPhylogeny
 
dc.subject.meshPolymorphism, Genetic
 
dc.subject.meshProtein Conformation
 
dc.titleAntigenic profile of avian H5N1 viruses in Asia from 2002 to 2007
 
dc.typeArticle
 
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Author Affiliations
  1. Xiamen University
  2. The University of Hong Kong
  3. Shantou University
  4. St. Jude Children Research Hospital