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Article: Molecular characterization of clinical isolates of Mycobacterium tuberculosis and their association with phenotypic virulence in human macrophages

TitleMolecular characterization of clinical isolates of Mycobacterium tuberculosis and their association with phenotypic virulence in human macrophages
Authors
Issue Date2007
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
Citation
Clinical And Vaccine Immunology, 2007, v. 14 n. 10, p. 1279-1284 How to Cite?
AbstractAmong 125 clinical isolates of Mycobacterium tuberculosis collected in Hong Kong and Shanghai, China, between 2002 and 2004, IS6110 typing revealed that 71 strains (57%) belonged to the Beijing family. The intracellular growth of the strains in human peripheral blood monocyte-derived macrophages was measured ex vivo on days 0, 3, 6, and 10. Among all tested strains, three hypervirulent strains showed significant increases in intracellular growth after 10 days of incubation. With an initial bacterial load of 10 4 CFU, most of the clinical isolates and H37Ra (an avirulent strain) exhibited no intracellular survival on day 10, while the three hypervirulent strains together with H37Rv (a virulent strain) showed on average a two- to fourfold rise in CFU count. These three hypervirulent strains belonging to a non-Beijing family were isolated from patients suffering from tuberculosis meningitis. Cytokines secreted by gamma interferon-activated macrophages were measured daily after challenge with selected strains of M. tuberculosis. The levels of tumor necrosis factor alpha were elevated after 24 h of infection among all strains, but the levels were significantly lower among the three hypervirulent strains, whereas interleukin 10 (IL-10) and IL-12 were not detected. Results were concordant with the differential expression of the corresponding cytokine genes in activated macrophages, as monitored by real-time PCR. Our findings highlighted that these three hypervirulent strains may possess an innate mechanism for escaping host immunity, which accounts for their characteristic virulence in patients presenting with a more severe form of disease. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157494
ISSN
2015 Impact Factor: 2.277
2015 SCImago Journal Rankings: 1.329
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, KCen_US
dc.contributor.authorLeong, WMen_US
dc.contributor.authorLaw, HKWen_US
dc.contributor.authorIp, KFen_US
dc.contributor.authorLam, JTHen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorHo, PLen_US
dc.contributor.authorTse, WSen_US
dc.contributor.authorWeng, XHen_US
dc.contributor.authorZhang, WHen_US
dc.contributor.authorChen, Sen_US
dc.contributor.authorYam, WCen_US
dc.date.accessioned2012-08-08T08:50:33Z-
dc.date.available2012-08-08T08:50:33Z-
dc.date.issued2007en_US
dc.identifier.citationClinical And Vaccine Immunology, 2007, v. 14 n. 10, p. 1279-1284en_US
dc.identifier.issn1556-6811en_US
dc.identifier.urihttp://hdl.handle.net/10722/157494-
dc.description.abstractAmong 125 clinical isolates of Mycobacterium tuberculosis collected in Hong Kong and Shanghai, China, between 2002 and 2004, IS6110 typing revealed that 71 strains (57%) belonged to the Beijing family. The intracellular growth of the strains in human peripheral blood monocyte-derived macrophages was measured ex vivo on days 0, 3, 6, and 10. Among all tested strains, three hypervirulent strains showed significant increases in intracellular growth after 10 days of incubation. With an initial bacterial load of 10 4 CFU, most of the clinical isolates and H37Ra (an avirulent strain) exhibited no intracellular survival on day 10, while the three hypervirulent strains together with H37Rv (a virulent strain) showed on average a two- to fourfold rise in CFU count. These three hypervirulent strains belonging to a non-Beijing family were isolated from patients suffering from tuberculosis meningitis. Cytokines secreted by gamma interferon-activated macrophages were measured daily after challenge with selected strains of M. tuberculosis. The levels of tumor necrosis factor alpha were elevated after 24 h of infection among all strains, but the levels were significantly lower among the three hypervirulent strains, whereas interleukin 10 (IL-10) and IL-12 were not detected. Results were concordant with the differential expression of the corresponding cytokine genes in activated macrophages, as monitored by real-time PCR. Our findings highlighted that these three hypervirulent strains may possess an innate mechanism for escaping host immunity, which accounts for their characteristic virulence in patients presenting with a more severe form of disease. Copyright © 2007, American Society for Microbiology. All Rights Reserved.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/en_US
dc.relation.ispartofClinical and Vaccine Immunologyen_US
dc.rightsClinical and Vaccine Immunology. Copyright © American Society for Microbiology.-
dc.subject.meshCytokines - Biosynthesis - Genetics - Secretionen_US
dc.subject.meshGene Expression Regulation, Bacterial - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMacrophages - Immunology - Microbiology - Secretionen_US
dc.subject.meshMycobacterium Tuberculosis - Genetics - Immunology - Isolation & Purification - Pathogenicityen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshVirulenceen_US
dc.titleMolecular characterization of clinical isolates of Mycobacterium tuberculosis and their association with phenotypic virulence in human macrophagesen_US
dc.typeArticleen_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_US
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.identifier.authorityHo, PL=rp00406en_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/CVI.00190-07en_US
dc.identifier.pmid17715326-
dc.identifier.scopuseid_2-s2.0-35549008076en_US
dc.identifier.hkuros137503-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35549008076&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume14en_US
dc.identifier.issue10en_US
dc.identifier.spage1279en_US
dc.identifier.epage1284en_US
dc.identifier.isiWOS:000250164800005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, KC=7404759813en_US
dc.identifier.scopusauthoridLeong, WM=22941186900en_US
dc.identifier.scopusauthoridLaw, HKW=7101939394en_US
dc.identifier.scopusauthoridIp, KF=22940676400en_US
dc.identifier.scopusauthoridLam, JTH=22941763000en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridTse, WS=35992535500en_US
dc.identifier.scopusauthoridWeng, XH=7102593970en_US
dc.identifier.scopusauthoridZhang, WH=15046133300en_US
dc.identifier.scopusauthoridChen, S=15831031400en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US

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