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Article: Preparation and Identification of a Single-chain Antibody Fragment Against High Pathogenic H5N1 Avian Influenza Virus

TitlePreparation and Identification of a Single-chain Antibody Fragment Against High Pathogenic H5N1 Avian Influenza Virus
Authors
Keywordshemagglution inhibition
high pathogenic avian influenza virus
neutralization test
single-chain fragment
Issue Date2007
Citation
Chinese Journal Of Biotechnology, 2007, v. 23 n. 2, p. 292-296 How to Cite?
AbstractPreviously, an mAb 10F7 was developed against H5N1 hemagglutinin, which was highly specific to 34 different H5N1 strains and showed good neutralizing activity. In the present study, the single-chain fragment of the antibody was cloned into a prokaryotic vector and then expressed in E. coli. The activity of the scFv was tested in hemagglution inhibition and neutralization experiment. Two H5N1 virus strains were inhibited to bind erythrocyte cells by the scFv while the H9 virus was not. Also, five H5N1 virus strains were neutralized during infecting MDCK cells. These results showed an approachable method for developing therapeutic antibody to H5N1 virus. © 2007 Institute of Microbiology, Chinese Academy of Sciences and Chinese Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/157480
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorFANG, Zen_HK
dc.contributor.authorLUO, WXen_HK
dc.contributor.authorWANG, MQen_HK
dc.contributor.authorCHEN, YWen_HK
dc.contributor.authorZHANG, Jen_HK
dc.contributor.authorCHEN, HLen_HK
dc.contributor.authorGUAN, Yen_HK
dc.contributor.authorXIA, NSen_HK
dc.date.accessioned2012-08-08T08:50:22Z-
dc.date.available2012-08-08T08:50:22Z-
dc.date.issued2007en_HK
dc.identifier.citationChinese Journal Of Biotechnology, 2007, v. 23 n. 2, p. 292-296en_HK
dc.identifier.issn1872-2075en_HK
dc.identifier.urihttp://hdl.handle.net/10722/157480-
dc.description.abstractPreviously, an mAb 10F7 was developed against H5N1 hemagglutinin, which was highly specific to 34 different H5N1 strains and showed good neutralizing activity. In the present study, the single-chain fragment of the antibody was cloned into a prokaryotic vector and then expressed in E. coli. The activity of the scFv was tested in hemagglution inhibition and neutralization experiment. Two H5N1 virus strains were inhibited to bind erythrocyte cells by the scFv while the H9 virus was not. Also, five H5N1 virus strains were neutralized during infecting MDCK cells. These results showed an approachable method for developing therapeutic antibody to H5N1 virus. © 2007 Institute of Microbiology, Chinese Academy of Sciences and Chinese Society for Microbiology.en_HK
dc.languageengen_US
dc.relation.ispartofChinese Journal of Biotechnologyen_HK
dc.subjecthemagglution inhibitionen_HK
dc.subjecthigh pathogenic avian influenza virusen_HK
dc.subjectneutralization testen_HK
dc.subjectsingle-chain fragmenten_HK
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Viral - Genetics - Immunology - Metabolismen_US
dc.subject.meshAntibody Specificity - Immunologyen_US
dc.subject.meshBirds - Virologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshInfluenza A Virus, H5n1 Subtype - Immunologyen_US
dc.subject.meshInfluenza In Birds - Virologyen_US
dc.subject.meshNeutralization Testsen_US
dc.subject.meshRecombinant Proteins - Immunology - Metabolismen_US
dc.subject.meshSingle-Chain Antibodies - Genetics - Immunology - Metabolismen_US
dc.titlePreparation and Identification of a Single-chain Antibody Fragment Against High Pathogenic H5N1 Avian Influenza Virusen_HK
dc.typeArticleen_HK
dc.identifier.emailCHEN, HL: hlchen@hku.hken_HK
dc.identifier.emailGUAN, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityCHEN, HL=rp00383en_HK
dc.identifier.authorityGUAN, Y=rp00397en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S1872-2075(07)60026-0en_HK
dc.identifier.pmid17460904-
dc.identifier.scopuseid_2-s2.0-34248647588en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34248647588&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue2en_HK
dc.identifier.spage292en_HK
dc.identifier.epage296en_HK
dc.identifier.scopusauthoridFANG, Z=36165347000en_HK
dc.identifier.scopusauthoridLUO, WX=7202199022en_HK
dc.identifier.scopusauthoridWANG, MQ=16314645900en_HK
dc.identifier.scopusauthoridCHEN, YW=8705548300en_HK
dc.identifier.scopusauthoridZHANG, J=35202802400en_HK
dc.identifier.scopusauthoridCHEN, HL=26643315400en_HK
dc.identifier.scopusauthoridGUAN, Y=7202924055en_HK
dc.identifier.scopusauthoridXIA, NS=35187953700en_HK

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