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Article: Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus

TitleNatural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus
Authors
Issue Date2007
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2007, v. 81 n. 9, p. 4694-4700 How to Cite?
AbstractThe severe acute respiratory syndrome (SARS) outbreak of 2002 and 2003 occurred as a result of zoonotic transmission. Coronavirus (CoV) found in naturally infected palm civet (civet-CoV) represents the closest genetic relative to SARS-CoV, but the degree and the determinants of cross-neutralization among these viruses remain to be investigated. Studies indicate that the receptor binding domain (RBD) of the SARS-CoV spike (S) glycoprotein contains major determinants for viral entry and neutralization. We aim to characterize the impact of natural mutations within the RBDs of civet-CoVs on viral entry and cross-neutralization. In this study, the S glycoprotein genes were recovered from naturally infected civets in central China (Hubei province), extending the geographic distribution of civet-CoV beyond the southeastern province of Guangdong. Moreover, pseudoviruses generated in our laboratory with four civet S genes, each with a distinct RBD, infected cells expressing human receptor angiotensin-converting enzyme 2, but with 90 to 95% less efficiency compared to that of SARS-CoV. These four civet S genes were also constructed as DNA vaccines to immunize mice. Immunized sera elicited against most civet S glycoproteins displayed potent neutralizing activities against autologous viruses but were much less efficient (50% inhibitory concentration, 20- to 40-fold) at neutralizing SARS-CoV and vice versa. Convalescence-phase sera from humans were similarly ineffective against the dominant civet pseudovirus. Our findings suggest that the design of SARS vaccine should consider not only preventing the reemergence of SARS-CoV but also providing cross-protection, thus interrupting zoonotic transmission of a group of genetically divergent civet CoVs of broad geographic origin. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157479
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Len_US
dc.contributor.authorFang, Qen_US
dc.contributor.authorDeng, Fen_US
dc.contributor.authorWang, Hen_US
dc.contributor.authorYi, CEen_US
dc.contributor.authorBa, Len_US
dc.contributor.authorYu, Wen_US
dc.contributor.authorLin, RDen_US
dc.contributor.authorLi, Ten_US
dc.contributor.authorHu, Zen_US
dc.contributor.authorHo, DDen_US
dc.contributor.authorZhang, Len_US
dc.contributor.authorChen, Zen_US
dc.date.accessioned2012-08-08T08:50:22Z-
dc.date.available2012-08-08T08:50:22Z-
dc.date.issued2007en_US
dc.identifier.citationJournal Of Virology, 2007, v. 81 n. 9, p. 4694-4700en_US
dc.identifier.issn0022-538Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/157479-
dc.description.abstractThe severe acute respiratory syndrome (SARS) outbreak of 2002 and 2003 occurred as a result of zoonotic transmission. Coronavirus (CoV) found in naturally infected palm civet (civet-CoV) represents the closest genetic relative to SARS-CoV, but the degree and the determinants of cross-neutralization among these viruses remain to be investigated. Studies indicate that the receptor binding domain (RBD) of the SARS-CoV spike (S) glycoprotein contains major determinants for viral entry and neutralization. We aim to characterize the impact of natural mutations within the RBDs of civet-CoVs on viral entry and cross-neutralization. In this study, the S glycoprotein genes were recovered from naturally infected civets in central China (Hubei province), extending the geographic distribution of civet-CoV beyond the southeastern province of Guangdong. Moreover, pseudoviruses generated in our laboratory with four civet S genes, each with a distinct RBD, infected cells expressing human receptor angiotensin-converting enzyme 2, but with 90 to 95% less efficiency compared to that of SARS-CoV. These four civet S genes were also constructed as DNA vaccines to immunize mice. Immunized sera elicited against most civet S glycoproteins displayed potent neutralizing activities against autologous viruses but were much less efficient (50% inhibitory concentration, 20- to 40-fold) at neutralizing SARS-CoV and vice versa. Convalescence-phase sera from humans were similarly ineffective against the dominant civet pseudovirus. Our findings suggest that the design of SARS vaccine should consider not only preventing the reemergence of SARS-CoV but also providing cross-protection, thus interrupting zoonotic transmission of a group of genetically divergent civet CoVs of broad geographic origin. Copyright © 2007, American Society for Microbiology. All Rights Reserved.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_US
dc.relation.ispartofJournal of Virologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChinaen_US
dc.subject.meshCluster Analysisen_US
dc.subject.meshCross Reactions - Genetics - Immunologyen_US
dc.subject.meshMembrane Glycoproteins - Genetics - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutation - Geneticsen_US
dc.subject.meshNeutralization Testsen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSars Virus - Geneticsen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshViral Envelope Proteins - Genetics - Metabolismen_US
dc.titleNatural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirusen_US
dc.typeArticleen_US
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/JVI.02389-06en_US
dc.identifier.pmid17314167-
dc.identifier.scopuseid_2-s2.0-34247647376en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247647376&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume81en_US
dc.identifier.issue9en_US
dc.identifier.spage4694en_US
dc.identifier.epage4700en_US
dc.identifier.eissn1098-5514-
dc.identifier.isiWOS:000246501900032-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLiu, L=36068379000en_US
dc.identifier.scopusauthoridFang, Q=55248545600en_US
dc.identifier.scopusauthoridDeng, F=35847541100en_US
dc.identifier.scopusauthoridWang, H=22942629300en_US
dc.identifier.scopusauthoridYi, CE=8557032800en_US
dc.identifier.scopusauthoridBa, L=8557032300en_US
dc.identifier.scopusauthoridYu, W=7403914045en_US
dc.identifier.scopusauthoridLin, RD=35074462700en_US
dc.identifier.scopusauthoridLi, T=8876653800en_US
dc.identifier.scopusauthoridHu, Z=7404210963en_US
dc.identifier.scopusauthoridHo, DD=7402971998en_US
dc.identifier.scopusauthoridZhang, L=8783285300en_US
dc.identifier.scopusauthoridChen, Z=35271180800en_US

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