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Article: Selection of a novel gp41-specific HIV-1 neutralizing human antibody by competitive antigen panning

TitleSelection of a novel gp41-specific HIV-1 neutralizing human antibody by competitive antigen panning
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
Citation
Journal Of Immunological Methods, 2006, v. 317 n. 1-2, p. 21-30 How to Cite?
AbstractThe HIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against Env-based antigens has resulted mostly in selection of anti-gp120 antibodies. Native gp41 in the absence of gp120 is unstable. The use of gp41 fragments as antigens has resulted in selection of antibodies with only relatively modest neutralizing activity. To enhance selection of antibodies specific for gp41 in the context of the whole Env we developed a methodology termed competitive antigen panning (CAP). Using CAP, we identified a novel gp41-specific human monoclonal antibody (hmAb), m48, from an immune library derived from long-term nonprogressors with high titers of broadly cross-reactive neutralizing antibodies (bcnAbs). Selection of m48 was only successful using CAP and not through the conventional pre-incubation methodology. In assays based on spreading infection in peripheral blood mononuclear cells (PBMCs) m48 neutralized a panel of HIV-1 primary isolates from different clades more potently than the well-characterized broadly cross-reactive HIV-1-neutralizing antibodies IgG1 4E10 and Fab Z13. These results may have implications for the selection of novel gp41-specific bcnAbs and other antibodies, and for the development of HIV-1 inhibitors and vaccine immunogens. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157478
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.053
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, MYen_US
dc.contributor.authorChoudhry, Ven_US
dc.contributor.authorSidorov, IAen_US
dc.contributor.authorTenev, Ven_US
dc.contributor.authorVu, BKen_US
dc.contributor.authorChoudhary, Aen_US
dc.contributor.authorLu, Hen_US
dc.contributor.authorStiegler, GMen_US
dc.contributor.authorKatinger, HWDen_US
dc.contributor.authorJiang, Sen_US
dc.contributor.authorBroder, CCen_US
dc.contributor.authorDimitrov, DSen_US
dc.date.accessioned2012-08-08T08:50:21Z-
dc.date.available2012-08-08T08:50:21Z-
dc.date.issued2006en_US
dc.identifier.citationJournal Of Immunological Methods, 2006, v. 317 n. 1-2, p. 21-30en_US
dc.identifier.issn0022-1759en_US
dc.identifier.urihttp://hdl.handle.net/10722/157478-
dc.description.abstractThe HIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against Env-based antigens has resulted mostly in selection of anti-gp120 antibodies. Native gp41 in the absence of gp120 is unstable. The use of gp41 fragments as antigens has resulted in selection of antibodies with only relatively modest neutralizing activity. To enhance selection of antibodies specific for gp41 in the context of the whole Env we developed a methodology termed competitive antigen panning (CAP). Using CAP, we identified a novel gp41-specific human monoclonal antibody (hmAb), m48, from an immune library derived from long-term nonprogressors with high titers of broadly cross-reactive neutralizing antibodies (bcnAbs). Selection of m48 was only successful using CAP and not through the conventional pre-incubation methodology. In assays based on spreading infection in peripheral blood mononuclear cells (PBMCs) m48 neutralized a panel of HIV-1 primary isolates from different clades more potently than the well-characterized broadly cross-reactive HIV-1-neutralizing antibodies IgG1 4E10 and Fab Z13. These results may have implications for the selection of novel gp41-specific bcnAbs and other antibodies, and for the development of HIV-1 inhibitors and vaccine immunogens. © 2006 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jimen_US
dc.relation.ispartofJournal of Immunological Methodsen_US
dc.subject.meshAntibodies, Monoclonal - Immunology - Isolation & Purificationen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshAntigens, Viral - Immunologyen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHiv Envelope Protein Gp41 - Immunologyen_US
dc.subject.meshHiv-1 - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunologic Techniquesen_US
dc.subject.meshNeutralization Tests - Methodsen_US
dc.subject.meshPeptide Libraryen_US
dc.titleSelection of a novel gp41-specific HIV-1 neutralizing human antibody by competitive antigen panningen_US
dc.typeArticleen_US
dc.identifier.emailZhang, MY:zhangmy@hku.hken_US
dc.identifier.authorityZhang, MY=rp01409en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jim.2006.09.016en_US
dc.identifier.pmid17078964-
dc.identifier.scopuseid_2-s2.0-34247569794en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247569794&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume317en_US
dc.identifier.issue1-2en_US
dc.identifier.spage21en_US
dc.identifier.epage30en_US
dc.identifier.isiWOS:000243148700003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhang, MY=35316639300en_US
dc.identifier.scopusauthoridChoudhry, V=8530897500en_US
dc.identifier.scopusauthoridSidorov, IA=7005186545en_US
dc.identifier.scopusauthoridTenev, V=15078280800en_US
dc.identifier.scopusauthoridVu, BK=7003942875en_US
dc.identifier.scopusauthoridChoudhary, A=8743351100en_US
dc.identifier.scopusauthoridLu, H=26642892200en_US
dc.identifier.scopusauthoridStiegler, GM=7003456168en_US
dc.identifier.scopusauthoridKatinger, HWD=7006456200en_US
dc.identifier.scopusauthoridJiang, S=7404453146en_US
dc.identifier.scopusauthoridBroder, CC=7004376461en_US
dc.identifier.scopusauthoridDimitrov, DS=7202564539en_US

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