Article: Catabacter hongkongensis gen. nov., sp. nov., isolated from blood cultures of patients from Hong Kong and Canada

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TitleCatabacter hongkongensis gen. nov., sp. nov., isolated from blood cultures of patients from Hong Kong and Canada
AuthorsLau, SKP1
Mcnabb, A3
Woo, GKS1
Hoang, L2 3
Fung, AMY1
Chung, LMW1
Woo, PCY1
Yuen, KY1
Issue Date2007
CitationJournal Of Clinical Microbiology, 2007, v. 45 n. 2, p. 395-401 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JCM.01831-06
AbstractFour bacterial isolates were recovered from the blood cultures of four patients, two of whom were from Hong Kong and two of whom were from Canada. The two Hong Kong strains were isolated from a 48-year-old man with intestinal obstruction and secondary sepsis (strain HKU16T) and from a 39-year-old man with acute appendicitis (strain HKU17), while the two Canadian strains were isolated from a 74-year-old man with biliary sepsis (strain CA1) and from a 66-year-old woman with metastatic carcinoma and sepsis (strain CA2). While the first three patients survived, the last patient died 2 weeks after the episode of bacteremia. All four isolates are strictly anaerobic, nonsporulating, gram-positive coccobacilli that were unidentified by conventional phenotypic tests and commercial identification systems. They grow on sheep blood agar as nonhemolytic pinpoint colonies after 48 h of incubation at 37°C in an anaerobic environment. All are catalase positive and motile, with flagella. They produce acid from arabinose, glucose, mannose, and xylose. They do not produce indole or reduce nitrate. They are sensitive to penicillin, vancomycin, and metronidazole but resistant to cefotaxime. 16S rRNA gene sequence analysis showed 16.0%, 16.8%, and 21.0% base differences from Clostridium propionicum, Clostridium neopropionicum, and Atopobium minutum, respectively. The G+C content of strain HKU16T is 40.2% ± 2.2%. Based on their phylogenetic affiliation, unique G+C content, and phenotypic characteristics, we propose a new genus and species, Catabacter hongkongensis gen. nov., sp. nov., to describe the bacterium, for which HKU16 is the type strain, and suggest that it be assigned to a new family, Catabacteriaceae. The gastrointestinal tract was probably the source of the bacterium for at least three of the four patients. The isolation of a catalase-positive, motile, nonsporulating, anaerobic gram-positive bacillus in clinical laboratories should raise the possibility of C. hongkongensis. Further studies should be performed to ascertain the epidemiology and other disease associations of this bacterium. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
ISSN0095-1137
2011 Impact Factor: 4.153
2011 SCImago Journal Rankings: 0.397
DOIhttp://dx.doi.org/10.1128/JCM.01831-06
ISI Accession Number IDWOS:000244270000022
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLau, SKP
dc.contributor.authorMcnabb, A
dc.contributor.authorWoo, GKS
dc.contributor.authorHoang, L
dc.contributor.authorFung, AMY
dc.contributor.authorChung, LMW
dc.contributor.authorWoo, PCY
dc.contributor.authorYuen, KY
dc.date.accessioned2012-08-08T08:50:15Z
dc.date.available2012-08-08T08:50:15Z
dc.date.issued2007
dc.description.abstractFour bacterial isolates were recovered from the blood cultures of four patients, two of whom were from Hong Kong and two of whom were from Canada. The two Hong Kong strains were isolated from a 48-year-old man with intestinal obstruction and secondary sepsis (strain HKU16T) and from a 39-year-old man with acute appendicitis (strain HKU17), while the two Canadian strains were isolated from a 74-year-old man with biliary sepsis (strain CA1) and from a 66-year-old woman with metastatic carcinoma and sepsis (strain CA2). While the first three patients survived, the last patient died 2 weeks after the episode of bacteremia. All four isolates are strictly anaerobic, nonsporulating, gram-positive coccobacilli that were unidentified by conventional phenotypic tests and commercial identification systems. They grow on sheep blood agar as nonhemolytic pinpoint colonies after 48 h of incubation at 37°C in an anaerobic environment. All are catalase positive and motile, with flagella. They produce acid from arabinose, glucose, mannose, and xylose. They do not produce indole or reduce nitrate. They are sensitive to penicillin, vancomycin, and metronidazole but resistant to cefotaxime. 16S rRNA gene sequence analysis showed 16.0%, 16.8%, and 21.0% base differences from Clostridium propionicum, Clostridium neopropionicum, and Atopobium minutum, respectively. The G+C content of strain HKU16T is 40.2% ± 2.2%. Based on their phylogenetic affiliation, unique G+C content, and phenotypic characteristics, we propose a new genus and species, Catabacter hongkongensis gen. nov., sp. nov., to describe the bacterium, for which HKU16 is the type strain, and suggest that it be assigned to a new family, Catabacteriaceae. The gastrointestinal tract was probably the source of the bacterium for at least three of the four patients. The isolation of a catalase-positive, motile, nonsporulating, anaerobic gram-positive bacillus in clinical laboratories should raise the possibility of C. hongkongensis. Further studies should be performed to ascertain the epidemiology and other disease associations of this bacterium. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Clinical Microbiology, 2007, v. 45 n. 2, p. 395-401 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JCM.01831-06
dc.identifier.doihttp://dx.doi.org/10.1128/JCM.01831-06
dc.identifier.epage401
dc.identifier.hkuros127781
dc.identifier.isiWOS:000244270000022
dc.identifier.issn0095-1137
2011 Impact Factor: 4.153
2011 SCImago Journal Rankings: 0.397
dc.identifier.issue2
dc.identifier.pmid17122022
dc.identifier.scopuseid_2-s2.0-33847022234
dc.identifier.spage395
dc.identifier.urihttp://hdl.handle.net/10722/157472
dc.identifier.volume45
dc.languageeng
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Clinical Microbiology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAnaerobiosis
dc.subject.meshBacteremia - Epidemiology - Microbiology
dc.subject.meshBacterial Typing Techniques
dc.subject.meshBase Composition
dc.subject.meshBlood - Microbiology
dc.subject.meshCanada - Epidemiology
dc.subject.meshCulture Media
dc.subject.meshFemale
dc.subject.meshGram-Positive Bacteria - Classification - Genetics - Isolation & Purification
dc.subject.meshGram-Positive Bacterial Infections - Epidemiology - Microbiology
dc.subject.meshHong Kong - Epidemiology
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshMolecular Sequence Data
dc.subject.meshPhenotype
dc.subject.meshPhylogeny
dc.subject.meshRna, Ribosomal, 16S - Genetics
dc.subject.meshSequence Analysis, Dna
dc.titleCatabacter hongkongensis gen. nov., sp. nov., isolated from blood cultures of patients from Hong Kong and Canada
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. The University of British Columbia
  3. BC Centre for Disease Control