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Article: Evaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia

TitleEvaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asia
Authors
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal of Clinical Virology, 2007, v. 38 n. 1, p. 39-43 How to Cite?
AbstractBackground: Monitoring anti-retroviral therapy requires that viral load assays for human immunodeficiency virus type 1 (HIV-1) be applicable to diverse HIV-1 subtypes. Objectives: To evaluate NucliSens EasyQ™ HIV-1 assay for quantitation of common HIV-1 subtypes prevalent in South-east Asia. Study design: One hundred and nineteen plasma samples collected in Hong Kong and Cambodia were used to compare the performance of NucliSens EasyQ™ HIV-1 and COBAS Amplicor™ HIV-1 Monitor version 1.5 assays. Viral RNA extracted from the NucliSens MiniMAG™ was also used for HIV-1 subtyping. Results: Performance of NucliSens EasyQ™ correlated well with COBAS Amplicor™ (r = 0.777, p < 0.001) and the small mean difference (0.0462 log10 IU/mL) obtained in the Bland and Altman model indicated good agreement between two assays. The NucliSens EasyQ™ assay demonstrated a 95% sensitivity at 500 IU/mL and 100% specificity. Reproducibility of this assay was within log10 2-4 IU/mL and had a coefficient of variation between 2.3% and 10.4%. Among the 109 specimens included in the analysis, HIV-1 subtyping identified 64 CRF01_AE, 38 subtype B, 3 subtype C, 3 CRF07_BC and 1 subtype G viruses. Conclusions: Performance of NucliSens EasyQ™ was comparable to COBAS Amplicor™ for HIV-1 viral load monitoring. RNA extracts from NucliSens MiniMAG™ could be used for HIV-1 viral load monitoring, subtyping and drug resistance mutations detection. Our findings highlight the versatility of both NucliSens EasyQ™ and COBAS Amplicor™ in monitoring prevalent subtypes and rare circulating recombinant forms (CRFs) in the South-east Asia region. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157464
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, HYen_US
dc.contributor.authorChen, JHKen_US
dc.contributor.authorWong, KHen_US
dc.contributor.authorChan, Ken_US
dc.contributor.authorLi, Pen_US
dc.contributor.authorLee, MPen_US
dc.contributor.authorTsang, DNen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorYam, WCen_US
dc.date.accessioned2012-08-08T08:50:10Z-
dc.date.available2012-08-08T08:50:10Z-
dc.date.issued2007en_US
dc.identifier.citationJournal of Clinical Virology, 2007, v. 38 n. 1, p. 39-43en_US
dc.identifier.issn1386-6532en_US
dc.identifier.urihttp://hdl.handle.net/10722/157464-
dc.description.abstractBackground: Monitoring anti-retroviral therapy requires that viral load assays for human immunodeficiency virus type 1 (HIV-1) be applicable to diverse HIV-1 subtypes. Objectives: To evaluate NucliSens EasyQ™ HIV-1 assay for quantitation of common HIV-1 subtypes prevalent in South-east Asia. Study design: One hundred and nineteen plasma samples collected in Hong Kong and Cambodia were used to compare the performance of NucliSens EasyQ™ HIV-1 and COBAS Amplicor™ HIV-1 Monitor version 1.5 assays. Viral RNA extracted from the NucliSens MiniMAG™ was also used for HIV-1 subtyping. Results: Performance of NucliSens EasyQ™ correlated well with COBAS Amplicor™ (r = 0.777, p < 0.001) and the small mean difference (0.0462 log10 IU/mL) obtained in the Bland and Altman model indicated good agreement between two assays. The NucliSens EasyQ™ assay demonstrated a 95% sensitivity at 500 IU/mL and 100% specificity. Reproducibility of this assay was within log10 2-4 IU/mL and had a coefficient of variation between 2.3% and 10.4%. Among the 109 specimens included in the analysis, HIV-1 subtyping identified 64 CRF01_AE, 38 subtype B, 3 subtype C, 3 CRF07_BC and 1 subtype G viruses. Conclusions: Performance of NucliSens EasyQ™ was comparable to COBAS Amplicor™ for HIV-1 viral load monitoring. RNA extracts from NucliSens MiniMAG™ could be used for HIV-1 viral load monitoring, subtyping and drug resistance mutations detection. Our findings highlight the versatility of both NucliSens EasyQ™ and COBAS Amplicor™ in monitoring prevalent subtypes and rare circulating recombinant forms (CRFs) in the South-east Asia region. © 2006 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_US
dc.relation.ispartofJournal of Clinical Virologyen_US
dc.rightsJournal of Clinical Virology. Copyright © Elsevier BV.-
dc.subject.meshCambodiaen_US
dc.subject.meshHIV Infections - Virologyen_US
dc.subject.meshHIV-1 - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshRna, Viral - Geneticsen_US
dc.subject.meshRandom Allocationen_US
dc.subject.meshReagent Kits, Diagnosticen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshViral Loaden_US
dc.titleEvaluation of NucliSens EasyQ™ HIV-1 assay for quantification of HIV-1 subtypes prevalent in South-east Asiaen_US
dc.typeArticleen_US
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_US
dc.identifier.emailYam, WC:w cyam@hkucc.hku.hken_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jcv.2006.10.002en_US
dc.identifier.pmid17110162-
dc.identifier.scopuseid_2-s2.0-33845287241en_US
dc.identifier.hkuros137463-
dc.identifier.hkuros142136-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845287241&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume38en_US
dc.identifier.issue1en_US
dc.identifier.spage39en_US
dc.identifier.epage43en_US
dc.identifier.isiWOS:000243736900007-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLam, HY=35097472400en_US
dc.identifier.scopusauthoridChen, JHK=12763271400en_US
dc.identifier.scopusauthoridWong, KH=7404758411en_US
dc.identifier.scopusauthoridChan, K=35097079800en_US
dc.identifier.scopusauthoridLi, P=36068280500en_US
dc.identifier.scopusauthoridLee, MP=7409121315en_US
dc.identifier.scopusauthoridTsang, DN=7005609132en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US
dc.customcontrol.immutablesml 130528-

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