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Article: MicroRNAs in human immunodeficiency virus-1 infection.

TitleMicroRNAs in human immunodeficiency virus-1 infection.
Authors
Issue Date2006
Citation
Methods In Molecular Biology (Clifton, N.J.), 2006, v. 342, p. 241-253 How to Cite?
AbstractInitially reported for Caenorhabditis elegans, microRNA (miRNA) has been shown to regulate gene expression in plants, flies, and mammals . Here, we report on our approaches to investigate the role of miRNAs in human immunodeficiency virus (HIV)-1 infection. Using computer-directed foldings, we first identify potential sequences in HIV-1 that putatively encode miRNAs. Subsequently, we use Northern blotting of RNAs isolated from HIV-infected cells to confirm expression of predicted miRNA sequences. Finally, we use a scanning algorithm to search 3' untranslated regions (UTRs) of human messenger RNAs (mRNAs) in the attempt to predict potential sites targeted by HIV-1 miRNAs.
Persistent Identifierhttp://hdl.handle.net/10722/157461
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorBennasser, Yen_US
dc.contributor.authorLe, SYen_US
dc.contributor.authorYeung, MLen_US
dc.contributor.authorJeang, KTen_US
dc.date.accessioned2012-08-08T08:50:09Z-
dc.date.available2012-08-08T08:50:09Z-
dc.date.issued2006en_US
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2006, v. 342, p. 241-253en_US
dc.identifier.issn1064-3745en_US
dc.identifier.urihttp://hdl.handle.net/10722/157461-
dc.description.abstractInitially reported for Caenorhabditis elegans, microRNA (miRNA) has been shown to regulate gene expression in plants, flies, and mammals . Here, we report on our approaches to investigate the role of miRNAs in human immunodeficiency virus (HIV)-1 infection. Using computer-directed foldings, we first identify potential sequences in HIV-1 that putatively encode miRNAs. Subsequently, we use Northern blotting of RNAs isolated from HIV-infected cells to confirm expression of predicted miRNA sequences. Finally, we use a scanning algorithm to search 3' untranslated regions (UTRs) of human messenger RNAs (mRNAs) in the attempt to predict potential sites targeted by HIV-1 miRNAs.en_US
dc.languageengen_US
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_US
dc.subject.meshAlgorithmsen_US
dc.subject.meshComputational Biology - Methodsen_US
dc.subject.meshHiv Infections - Virologyen_US
dc.subject.meshHiv-1 - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrornas - Chemistry - Genetics - Metabolismen_US
dc.subject.meshMicroarray Analysis - Methodsen_US
dc.titleMicroRNAs in human immunodeficiency virus-1 infection.en_US
dc.typeArticleen_US
dc.identifier.emailYeung, ML:pmlyeung@hku.hken_US
dc.identifier.authorityYeung, ML=rp01402en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid16957379-
dc.identifier.scopuseid_2-s2.0-33749048265en_US
dc.identifier.volume342en_US
dc.identifier.spage241en_US
dc.identifier.epage253en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBennasser, Y=8335747500en_US
dc.identifier.scopusauthoridLe, SY=7006184376en_US
dc.identifier.scopusauthoridYeung, ML=8350940900en_US
dc.identifier.scopusauthoridJeang, KT=7004824803en_US

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