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Article: HIV-1 TAR RNA subverts RNA interference in transfected cells through sequestration of TAR RNA-binding protein, TRBP

TitleHIV-1 TAR RNA subverts RNA interference in transfected cells through sequestration of TAR RNA-binding protein, TRBP
Authors
Issue Date2006
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2006, v. 281 n. 38, p. 27674-27678 How to Cite?
AbstractTAR RNA-binding protein, TRBP, was recently discovered to be an essential partner for Dicer and a crucial component of the RNA-induced silencing complex (RISC), a critical element of the RNA interference (RNAi) of the cell apparatus. Human TRBP was originally characterized and cloned 15 years ago based on its high affinity for binding the HIV-1 encoded leader RNA, TAR. RNAi is used, in part, by cells to defend against infection by viruses. Here, we report that transfected TAR RNA can attenuate the RNAi machinery in human cells. Our data suggest that TAR RNA sequesters TRBP rendering it unavailable for downstream Dicer-RISC complexes. TAR-induced inhibition of Dicer-RISC activity in transfected cells was partially relieved by exogenous expression of TRBP.
Persistent Identifierhttp://hdl.handle.net/10722/157460
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBennasser, Yen_US
dc.contributor.authorYeung, MLen_US
dc.contributor.authorJeang, KTen_US
dc.date.accessioned2012-08-08T08:50:08Z-
dc.date.available2012-08-08T08:50:08Z-
dc.date.issued2006en_US
dc.identifier.citationJournal Of Biological Chemistry, 2006, v. 281 n. 38, p. 27674-27678en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/157460-
dc.description.abstractTAR RNA-binding protein, TRBP, was recently discovered to be an essential partner for Dicer and a crucial component of the RNA-induced silencing complex (RISC), a critical element of the RNA interference (RNAi) of the cell apparatus. Human TRBP was originally characterized and cloned 15 years ago based on its high affinity for binding the HIV-1 encoded leader RNA, TAR. RNAi is used, in part, by cells to defend against infection by viruses. Here, we report that transfected TAR RNA can attenuate the RNAi machinery in human cells. Our data suggest that TAR RNA sequesters TRBP rendering it unavailable for downstream Dicer-RISC complexes. TAR-induced inhibition of Dicer-RISC activity in transfected cells was partially relieved by exogenous expression of TRBP.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshHiv Long Terminal Repeat - Physiologyen_US
dc.subject.meshHiv-1 - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshRna Interferenceen_US
dc.subject.meshRna, Small Interfering - Physiologyen_US
dc.subject.meshRna, Viral - Physiologyen_US
dc.subject.meshRna-Binding Proteins - Metabolism - Physiologyen_US
dc.subject.meshRibonuclease Iii - Antagonists & Inhibitorsen_US
dc.subject.meshTransfectionen_US
dc.titleHIV-1 TAR RNA subverts RNA interference in transfected cells through sequestration of TAR RNA-binding protein, TRBPen_US
dc.typeArticleen_US
dc.identifier.emailYeung, ML:pmlyeung@hku.hken_US
dc.identifier.authorityYeung, ML=rp01402en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.C600072200en_US
dc.identifier.pmid16887810-
dc.identifier.scopuseid_2-s2.0-33748775175en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748775175&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume281en_US
dc.identifier.issue38en_US
dc.identifier.spage27674en_US
dc.identifier.epage27678en_US
dc.identifier.isiWOS:000240534400003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBennasser, Y=8335747500en_US
dc.identifier.scopusauthoridYeung, ML=8350940900en_US
dc.identifier.scopusauthoridJeang, KT=7004824803en_US

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