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Article: A novel approach for screening immunogenic proteins in Penicillium marneffei using the ΔAFMP1ΔAFMP2 deletion mutant of Aspergillus fumigatus

TitleA novel approach for screening immunogenic proteins in Penicillium marneffei using the ΔAFMP1ΔAFMP2 deletion mutant of Aspergillus fumigatus
Authors
Issue Date2006
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1
Citation
FEMS Microbiology Letters, 2006, v. 262 n. 2, p. 138-147 How to Cite?
AbstractUsing serum from guinea-pigs immunized with a ΔAFMP1ΔAFMP2 deletion mutant of Aspergillus fumigatus to screen a cDNA library of A. fumigatus, we cloned a novel immunogenic 57-kDa protein in A. fumigatus. We also cloned its 55-kDa homologue in Penicillium marneffei, which was possibly related to amino acid biosynthesis and metabolism, with homologues present only in the subphylum Pezizomycotina of Ascomycota. The recombinant 55-kDa protein of P. marneffei reacted strongly with guinea-pig serum immunized with P. marneffei and with the sera of patients with P. marneffei infection. A similar approach could be applied to immunogenic protein screening in other microorganisms for serological diagnosis, epidemiological studies and the study of vaccines. © 2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157456
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.126
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorChong, KTKen_US
dc.contributor.authorLau, CCYen_US
dc.contributor.authorWong, SSYen_US
dc.contributor.authorLau, SKPen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:50:07Z-
dc.date.available2012-08-08T08:50:07Z-
dc.date.issued2006en_US
dc.identifier.citationFEMS Microbiology Letters, 2006, v. 262 n. 2, p. 138-147en_US
dc.identifier.issn0378-1097en_US
dc.identifier.urihttp://hdl.handle.net/10722/157456-
dc.description.abstractUsing serum from guinea-pigs immunized with a ΔAFMP1ΔAFMP2 deletion mutant of Aspergillus fumigatus to screen a cDNA library of A. fumigatus, we cloned a novel immunogenic 57-kDa protein in A. fumigatus. We also cloned its 55-kDa homologue in Penicillium marneffei, which was possibly related to amino acid biosynthesis and metabolism, with homologues present only in the subphylum Pezizomycotina of Ascomycota. The recombinant 55-kDa protein of P. marneffei reacted strongly with guinea-pig serum immunized with P. marneffei and with the sera of patients with P. marneffei infection. A similar approach could be applied to immunogenic protein screening in other microorganisms for serological diagnosis, epidemiological studies and the study of vaccines. © 2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1en_US
dc.relation.ispartofFEMS Microbiology Lettersen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Fungal - Immunologyen_US
dc.subject.meshAntigens, Fungal - Geneticsen_US
dc.subject.meshAspergillus Fumigatus - Genetics - Immunologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshBone Marrow Transplantationen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshFungal Proteins - Genetics - Immunology - Isolation & Purificationen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshGenes, Fungalen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunologic Techniquesen_US
dc.subject.meshMembrane Glycoproteins - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMycoses - Microbiologyen_US
dc.subject.meshPenicillium - Classification - Immunology - Isolation & Purificationen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshRecombinant Proteins - Immunology - Isolation & Purificationen_US
dc.titleA novel approach for screening immunogenic proteins in Penicillium marneffei using the ΔAFMP1ΔAFMP2 deletion mutant of Aspergillus fumigatusen_US
dc.typeArticleen_US
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_US
dc.identifier.emailWong, SSY: samsonsy@hkucc.hku.hken_US
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_US
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityWong, SSY=rp00395en_US
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1111/j.1574-6968.2006.00376.xen_US
dc.identifier.pmid16923068-
dc.identifier.scopuseid_2-s2.0-33747672026en_US
dc.identifier.hkuros120578-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33747672026&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume262en_US
dc.identifier.issue2en_US
dc.identifier.spage138en_US
dc.identifier.epage147en_US
dc.identifier.isiWOS:000239940400003-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridChong, KTK=7102553965en_US
dc.identifier.scopusauthoridLau, CCY=8398162900en_US
dc.identifier.scopusauthoridWong, SSY=13310021400en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.customcontrol.immutablesml 130529-

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