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Article: Sensitive and inexpensive molecular test for falciparum malaria: Defecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification

TitleSensitive and inexpensive molecular test for falciparum malaria: Defecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification
Authors
Issue Date2006
PublisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org
Citation
Clinical Chemistry, 2006, v. 52 n. 2, p. 303-306 How to Cite?
AbstractBackground: Malaria is one of the most important parasitic infections in humans. A sensitive diagnostic test for malaria that could be applied at the community level could be useful in programs to control the disease. The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum. Methods: Blood was collected from controls (n = 100) and from patients diagnosed with falciparum malaria infection (n = 102), who were recruited to the study. Heat-treated blood samples were tested by a loop-mediated isothermal amplification (LAMP) assay for P. falciparum. Results were interpreted by a turbidity meter in real time or visually at the end of the assay. To evaluate the assay, DNA from these samples was purified and tested by PCR. Results from the LAMP and PCR assays were compared. Results: The LAMP assay detected P. falciparum directly from heat-treated blood. The quantitative data from the assay correlated to the parasite counts obtained by blood-film microscopic analyses. When we used the PCR assay as the comparison method, the sensitivity and specificity of the LAMP assay were 95% and 99%, respectively. Conclusions: Unlike PCR, the LAMP assay does not require purified DNA for efficient DNA amplification, thereby reducing the cost and turnaround time for P. falciparum diagnosis. The assay requires only basic instruments, and assay positivity can be verified by visual inspection. © 2006 American Association for Clinical Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/157437
ISSN
2015 Impact Factor: 7.457
2015 SCImago Journal Rankings: 2.472
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorWong, BWYen_HK
dc.contributor.authorMa, EHTen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorChow, LMCen_HK
dc.contributor.authorAbeyewickreme, Wen_HK
dc.contributor.authorTangpukdee, Nen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorLooareesuwan, Sen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2012-08-08T08:49:58Z-
dc.date.available2012-08-08T08:49:58Z-
dc.date.issued2006en_HK
dc.identifier.citationClinical Chemistry, 2006, v. 52 n. 2, p. 303-306en_HK
dc.identifier.issn0009-9147en_HK
dc.identifier.urihttp://hdl.handle.net/10722/157437-
dc.description.abstractBackground: Malaria is one of the most important parasitic infections in humans. A sensitive diagnostic test for malaria that could be applied at the community level could be useful in programs to control the disease. The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum. Methods: Blood was collected from controls (n = 100) and from patients diagnosed with falciparum malaria infection (n = 102), who were recruited to the study. Heat-treated blood samples were tested by a loop-mediated isothermal amplification (LAMP) assay for P. falciparum. Results were interpreted by a turbidity meter in real time or visually at the end of the assay. To evaluate the assay, DNA from these samples was purified and tested by PCR. Results from the LAMP and PCR assays were compared. Results: The LAMP assay detected P. falciparum directly from heat-treated blood. The quantitative data from the assay correlated to the parasite counts obtained by blood-film microscopic analyses. When we used the PCR assay as the comparison method, the sensitivity and specificity of the LAMP assay were 95% and 99%, respectively. Conclusions: Unlike PCR, the LAMP assay does not require purified DNA for efficient DNA amplification, thereby reducing the cost and turnaround time for P. falciparum diagnosis. The assay requires only basic instruments, and assay positivity can be verified by visual inspection. © 2006 American Association for Clinical Chemistry.en_HK
dc.languageengen_US
dc.publisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.orgen_HK
dc.relation.ispartofClinical Chemistryen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCalibrationen_US
dc.subject.meshDna, Protozoan - Blood - Geneticsen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshHumansen_US
dc.subject.meshMalaria, Falciparum - Blood - Diagnosisen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlasmodium Falciparum - Genetics - Isolation & Purificationen_US
dc.subject.meshPolymerase Chain Reaction - Economics - Methods - Standardsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSpecimen Handling - Methodsen_US
dc.titleSensitive and inexpensive molecular test for falciparum malaria: Defecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplificationen_HK
dc.typeArticleen_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailWong, WY: wongwy5@HKUCC.hku.hken_HK
dc.identifier.emailChan, KH: chankh2@HKUCC.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1373/clinchem.2005.057901en_HK
dc.identifier.pmid16339303-
dc.identifier.scopuseid_2-s2.0-31844450172en_HK
dc.identifier.hkuros116637-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-31844450172&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue2en_HK
dc.identifier.spage303en_HK
dc.identifier.epage306en_HK
dc.identifier.isiWOS:000235069600019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridWong, BWY=8602085500en_HK
dc.identifier.scopusauthoridMa, EHT=12140611300en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridChow, LMC=7202533071en_HK
dc.identifier.scopusauthoridAbeyewickreme, W=12141290500en_HK
dc.identifier.scopusauthoridTangpukdee, N=8986273000en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridLooareesuwan, S=35411827400en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.customcontrol.immutablesml 130530-

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