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Article: Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library

TitleScreening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library
Authors
Issue Date2005
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/jcc
Citation
Journal Of Combinatorial Chemistry, 2005, v. 7 n. 5, p. 648-656 How to Cite?
AbstractA 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (∼66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S 471-503, a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S 471-503 peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion. © 2005 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/157423
ISSN
2012 Impact Factor: 4.933
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, Hen_US
dc.contributor.authorLi, Len_US
dc.contributor.authorKao, RYen_US
dc.contributor.authorKou, Ben_US
dc.contributor.authorWang, Zen_US
dc.contributor.authorZhang, Len_US
dc.contributor.authorZhang, Hen_US
dc.contributor.authorHao, Zen_US
dc.contributor.authorTsui, WHen_US
dc.contributor.authorNi, Aen_US
dc.contributor.authorCui, Len_US
dc.contributor.authorFan, Ben_US
dc.contributor.authorGuo, Fen_US
dc.contributor.authorRao, Sen_US
dc.contributor.authorJiang, Cen_US
dc.contributor.authorLi, Qen_US
dc.contributor.authorSun, Men_US
dc.contributor.authorHe, Wen_US
dc.contributor.authorLiu, Gen_US
dc.date.accessioned2012-08-08T08:49:50Z-
dc.date.available2012-08-08T08:49:50Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Combinatorial Chemistry, 2005, v. 7 n. 5, p. 648-656en_US
dc.identifier.issn1520-4766en_US
dc.identifier.urihttp://hdl.handle.net/10722/157423-
dc.description.abstractA 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (∼66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S 471-503, a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S 471-503 peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion. © 2005 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/jccen_US
dc.relation.ispartofJournal of Combinatorial Chemistryen_US
dc.titleScreening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide libraryen_US
dc.typeArticleen_US
dc.identifier.emailKao, RY:rytkao@hkucc.hku.hken_US
dc.identifier.authorityKao, RY=rp00481en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/cc0500607en_US
dc.identifier.scopuseid_2-s2.0-27744442815en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27744442815&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume7en_US
dc.identifier.issue5en_US
dc.identifier.spage648en_US
dc.identifier.epage656en_US
dc.identifier.isiWOS:000231871600004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHu, H=8957660800en_US
dc.identifier.scopusauthoridLi, L=36064664900en_US
dc.identifier.scopusauthoridKao, RY=7101675499en_US
dc.identifier.scopusauthoridKou, B=6701731246en_US
dc.identifier.scopusauthoridWang, Z=36077454600en_US
dc.identifier.scopusauthoridZhang, L=8677295200en_US
dc.identifier.scopusauthoridZhang, H=9844827400en_US
dc.identifier.scopusauthoridHao, Z=7201992661en_US
dc.identifier.scopusauthoridTsui, WH=36124344600en_US
dc.identifier.scopusauthoridNi, A=7004451149en_US
dc.identifier.scopusauthoridCui, L=7201555656en_US
dc.identifier.scopusauthoridFan, B=7102879209en_US
dc.identifier.scopusauthoridGuo, F=35227305400en_US
dc.identifier.scopusauthoridRao, S=9845104200en_US
dc.identifier.scopusauthoridJiang, C=35076200700en_US
dc.identifier.scopusauthoridLi, Q=36080215300en_US
dc.identifier.scopusauthoridSun, M=7403181348en_US
dc.identifier.scopusauthoridHe, W=7402007628en_US
dc.identifier.scopusauthoridLiu, G=8833437700en_US

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