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Article: Natural infection of a household pet red-capped mangabey (Cercocebus torquatus torquatus) with a new simian immunodeficiency virus

TitleNatural infection of a household pet red-capped mangabey (Cercocebus torquatus torquatus) with a new simian immunodeficiency virus
Authors
Issue Date1998
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 1998, v. 72 n. 1, p. 600-608 How to Cite?
AbstractA seroprevalence survey was conducted for simian immunodeficiency virus (SIV) antibody in household pet monkeys in Gabon. Twenty-nine monkeys representing seven species were analyzed. By using human immunodeficiency virus type 2 (HIV-2)/SIVsm, SIVmnd, and SIVagm antigens, one red-capped mangabey (RCM) (Cercocebus torquatus torquatus) was identified as harboring SIV-cross-reactive antibodies. A virus isolate, termed SIVrcm, was subsequently established from this seropositive RCM by cocultivation of its peripheral blood mononuclear cells (PBMC) with PBMC from seronegative humans or RCMs. SIVrcm was also isolated by cocultivation of CD8-depleted RCM PBMC with Molt 4 clone 8 cells but not with CEMx174 cells. The lack of growth in CEMx174 cells distinguished this new SIV from all previously reported sooty mangabey-derived viruses (SIVsm), which grow well in this cell line. SIVrcm was also successfully transmitted (cell free) to human and rhesus PBMC as well as to Molt 4 clone 8 cells. To determine the evolutionary origins of this newly identified virus, subgenomic pol (475 bp) and gag (954 bp) gene fragments were amplified from infected cell culture DNA and sequenced. The position of SIVrcm relative to those of members of the other primate lentivirus lineages was then examined in evolutionary trees constructed from deduced protein sequences. This analysis revealed significantly discordant phylogenetic positions of SIVrcm in the two genomic regions. In trees derived from partial gag sequences, SIVrcm clustered independently from all other HIV and SIV strains, consistent with a new primate lentivirus lineage. However, in trees derived from pol sequences, SIVrcm grouped with the HIV-I/SIVcpz lineage. These findings suggest that the SIVrcm genome is mosaic and possibly is the result of a recombination event involving divergent lentiviruses in the distant past. Further analysis of this and other SIVrcm isolates may shed new light on the origin of HIV-1.
Persistent Identifierhttp://hdl.handle.net/10722/157419
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGeorgesCourbot, MCen_US
dc.contributor.authorLu, CYen_US
dc.contributor.authorMakuwa, Men_US
dc.contributor.authorTelfer, Pen_US
dc.contributor.authorOnanga, Ren_US
dc.contributor.authorDubreuil, Gen_US
dc.contributor.authorChen, Zen_US
dc.contributor.authorSmith, SMen_US
dc.contributor.authorGeorges, Aen_US
dc.contributor.authorGao, Fen_US
dc.contributor.authorHahn, BHen_US
dc.contributor.authorMarx, PAen_US
dc.date.accessioned2012-08-08T08:49:47Z-
dc.date.available2012-08-08T08:49:47Z-
dc.date.issued1998en_US
dc.identifier.citationJournal Of Virology, 1998, v. 72 n. 1, p. 600-608en_US
dc.identifier.issn0022-538Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/157419-
dc.description.abstractA seroprevalence survey was conducted for simian immunodeficiency virus (SIV) antibody in household pet monkeys in Gabon. Twenty-nine monkeys representing seven species were analyzed. By using human immunodeficiency virus type 2 (HIV-2)/SIVsm, SIVmnd, and SIVagm antigens, one red-capped mangabey (RCM) (Cercocebus torquatus torquatus) was identified as harboring SIV-cross-reactive antibodies. A virus isolate, termed SIVrcm, was subsequently established from this seropositive RCM by cocultivation of its peripheral blood mononuclear cells (PBMC) with PBMC from seronegative humans or RCMs. SIVrcm was also isolated by cocultivation of CD8-depleted RCM PBMC with Molt 4 clone 8 cells but not with CEMx174 cells. The lack of growth in CEMx174 cells distinguished this new SIV from all previously reported sooty mangabey-derived viruses (SIVsm), which grow well in this cell line. SIVrcm was also successfully transmitted (cell free) to human and rhesus PBMC as well as to Molt 4 clone 8 cells. To determine the evolutionary origins of this newly identified virus, subgenomic pol (475 bp) and gag (954 bp) gene fragments were amplified from infected cell culture DNA and sequenced. The position of SIVrcm relative to those of members of the other primate lentivirus lineages was then examined in evolutionary trees constructed from deduced protein sequences. This analysis revealed significantly discordant phylogenetic positions of SIVrcm in the two genomic regions. In trees derived from partial gag sequences, SIVrcm clustered independently from all other HIV and SIV strains, consistent with a new primate lentivirus lineage. However, in trees derived from pol sequences, SIVrcm grouped with the HIV-I/SIVcpz lineage. These findings suggest that the SIVrcm genome is mosaic and possibly is the result of a recombination event involving divergent lentiviruses in the distant past. Further analysis of this and other SIVrcm isolates may shed new light on the origin of HIV-1.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_US
dc.relation.ispartofJournal of Virologyen_US
dc.subject.meshAdaptation, Physiologicalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Domestic - Virologyen_US
dc.subject.meshAntibodies, Viral - Blooden_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCercocebus - Virologyen_US
dc.subject.meshCross Reactionsen_US
dc.subject.meshDna Primers - Geneticsen_US
dc.subject.meshEvolution, Molecularen_US
dc.subject.meshFemaleen_US
dc.subject.meshGabonen_US
dc.subject.meshGenes, Gagen_US
dc.subject.meshGenes, Polen_US
dc.subject.meshHiv-2 - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes, Mononuclear - Virologyen_US
dc.subject.meshMacaca Mulattaen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshSimian Acquired Immunodeficiency Syndrome - Etiology - Immunology - Virologyen_US
dc.subject.meshSimian Immunodeficiency Virus - Genetics - Immunology - Isolation & Purificationen_US
dc.subject.meshVirus Cultivationen_US
dc.titleNatural infection of a household pet red-capped mangabey (Cercocebus torquatus torquatus) with a new simian immunodeficiency virusen_US
dc.typeArticleen_US
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9420264-
dc.identifier.scopuseid_2-s2.0-2642706680en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2642706680&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume72en_US
dc.identifier.issue1en_US
dc.identifier.spage600en_US
dc.identifier.epage608en_US
dc.identifier.isiWOS:A1998YL01000071-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGeorgesCourbot, MC=24431741900en_US
dc.identifier.scopusauthoridLu, CY=8777225600en_US
dc.identifier.scopusauthoridMakuwa, M=6603811063en_US
dc.identifier.scopusauthoridTelfer, P=36907430100en_US
dc.identifier.scopusauthoridOnanga, R=6507985453en_US
dc.identifier.scopusauthoridDubreuil, G=6701317912en_US
dc.identifier.scopusauthoridChen, Z=35271180800en_US
dc.identifier.scopusauthoridSmith, SM=35444937400en_US
dc.identifier.scopusauthoridGeorges, A=7103046876en_US
dc.identifier.scopusauthoridGao, F=34769702200en_US
dc.identifier.scopusauthoridHahn, BH=7201799232en_US
dc.identifier.scopusauthoridMarx, PA=7102894750en_US

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