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Article: Preparation and characterization of monoclonal antibodies against S1 domain at N-terminal residues 249 to 667 of SARS-associated coronavirus S1 protein.

TitlePreparation and characterization of monoclonal antibodies against S1 domain at N-terminal residues 249 to 667 of SARS-associated coronavirus S1 protein.
Authors
Issue Date2004
Publisher第一軍醫大學. The Journal's web site is located at http://dyjd.chinajournal.net.cn/
Citation
Di Yi Jun Yi Da Xue Xue Bao = Academic Journal Of The First Medical College Of Pla, 2004, v. 24 n. 1, p. 1-6 How to Cite?
AbstractOBJECTIVE: To prepare and characterize monoclonal antibodies (mAbs) against S1 protein of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). METHODS: 6-His-tagged recombinant fragment at N-terminal residues 249 to 667 of SARS-CoV S1 protein including S-protein receptor-binding domain was expressed in E.coli. The immunogenicity of this S1 domain was identified and used to immunize BALB/c mice for the production of hybridomas. The identification of the mAbs against this S1 domain was performed using indirect enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA) and Western blotting, respectively. RESULTS: Three hybridomas producing mAbs specific to the S1 domain was obtained, with a relative molecular mass of 48,500. None of the 3 mAbs were reactive with human coronaviruses 229E and OC43. Two of the mAbs were IgG2a isotype, and the other was IgG1. CONCLUSIONS: This is the first report of mAbs produced against S-protein receptor-binding domain of SARS-CoV. The 3 S1-specific mAbs may be useful for further study of the function of the S protein and for diagnosis of SARS-CoV infection.
Persistent Identifierhttp://hdl.handle.net/10722/157413
ISSN

 

DC FieldValueLanguage
dc.contributor.authorWen, Ken_US
dc.contributor.authorMei, YBen_US
dc.contributor.authorQiu, LWen_US
dc.contributor.authorLiao, ZYen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorChe, XYen_US
dc.date.accessioned2012-08-08T08:49:45Z-
dc.date.available2012-08-08T08:49:45Z-
dc.date.issued2004en_US
dc.identifier.citationDi Yi Jun Yi Da Xue Xue Bao = Academic Journal Of The First Medical College Of Pla, 2004, v. 24 n. 1, p. 1-6en_US
dc.identifier.issn1000-2588en_US
dc.identifier.urihttp://hdl.handle.net/10722/157413-
dc.description.abstractOBJECTIVE: To prepare and characterize monoclonal antibodies (mAbs) against S1 protein of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). METHODS: 6-His-tagged recombinant fragment at N-terminal residues 249 to 667 of SARS-CoV S1 protein including S-protein receptor-binding domain was expressed in E.coli. The immunogenicity of this S1 domain was identified and used to immunize BALB/c mice for the production of hybridomas. The identification of the mAbs against this S1 domain was performed using indirect enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA) and Western blotting, respectively. RESULTS: Three hybridomas producing mAbs specific to the S1 domain was obtained, with a relative molecular mass of 48,500. None of the 3 mAbs were reactive with human coronaviruses 229E and OC43. Two of the mAbs were IgG2a isotype, and the other was IgG1. CONCLUSIONS: This is the first report of mAbs produced against S-protein receptor-binding domain of SARS-CoV. The 3 S1-specific mAbs may be useful for further study of the function of the S protein and for diagnosis of SARS-CoV infection.en_US
dc.languageengen_US
dc.publisher第一軍醫大學. The Journal's web site is located at http://dyjd.chinajournal.net.cn/en_US
dc.relation.ispartofDi yi jun yi da xue xue bao = Academic journal of the First Medical College of PLAen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Biosynthesis - Immunologyen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Lineen_US
dc.subject.meshFemaleen_US
dc.subject.meshFluorescent Antibody Technique, Indirecten_US
dc.subject.meshHumansen_US
dc.subject.meshMembrane Glycoproteins - Immunology - Physiologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshPeptide Fragments - Immunologyen_US
dc.subject.meshSars Virus - Chemistryen_US
dc.subject.meshSevere Acute Respiratory Syndrome - Diagnosisen_US
dc.subject.meshViral Envelope Proteins - Immunology - Physiologyen_US
dc.titlePreparation and characterization of monoclonal antibodies against S1 domain at N-terminal residues 249 to 667 of SARS-associated coronavirus S1 protein.en_US
dc.typeArticleen_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid14724082-
dc.identifier.scopuseid_2-s2.0-2342660724en_US
dc.identifier.hkuros87949-
dc.identifier.volume24en_US
dc.identifier.issue1en_US
dc.identifier.spage1en_US
dc.identifier.epage6en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWen, K=8231190100en_US
dc.identifier.scopusauthoridMei, YB=8231190400en_US
dc.identifier.scopusauthoridQiu, LW=8719102700en_US
dc.identifier.scopusauthoridLiao, ZY=7203032864en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US
dc.identifier.scopusauthoridChe, XY=7005743182en_US

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