Article: Occurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002

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TitleOccurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002
AuthorsHo, PL1
Ho, AYM1
Chow, KH1
Wong, RCW1
Duan, RS1
Ho, WL1
Mak, GC1
Tsang, KW1
Yam, WC1
Yuen, KY1
Issue Date2005
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
CitationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 6, p. 840-845 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dki135
AbstractObjectives: A study was conducted to evaluate the occurrence and characterization of extended-spectrum β-lactamases (ESBLs) among blood isolates of Proteus mirabilis collected over a 4 year period in Hong Kong. Methods: Production of ESBLs among 99 consecutive and non-duplicate isolates was evaluated by the double-disc synergy test. The ESBLs were characterized by isoelectric focusing and PCR sequencing using specific primers. The epidemiological relationship of the isolates was studied by the Dienes test and PFGE. Results: ESBLs were identified in 13 isolates, from none in 1999-2000 and up to 18.5% (5/27) in 2001 and 25.8% (8/31) in 2002. The ESBL-producing isolates were more resistant to ceftriaxone than to ceftazidime, and were more likely than non-ESBL-producers to have resistance to ciprofloxacin (76.9% versus 14%) and gentamicin (38.5% versus 9.3%). The ESBL content included CTX-M-13 (n=8), CTX-M-14 (n=3), SHV-5 (n=2), TEM-11 (n=1), and an unidentified ESBL with a pI of 7.5. The Dienes test revealed that the genetic background in the 99 isolates was highly heterogeneous, with 54 distinct types among 92 isolates and seven were non-typeable. Among the 13 ESBL-producing isolates, five different backgrounds, including one cluster (Dienes-pulsotype A) with nine isolates, were identified by both Dienes test and PFGE, thus suggesting both clonal and multi-clonal spread of the CTX-M enzymes. Conclusions: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition. © The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
ISSN0305-7453
2011 Impact Factor: 5.068
2011 SCImago Journal Rankings: 0.422
DOIhttp://dx.doi.org/10.1093/jac/dki135
ISI Accession Number IDWOS:000230028100007
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHo, PL
dc.contributor.authorHo, AYM
dc.contributor.authorChow, KH
dc.contributor.authorWong, RCW
dc.contributor.authorDuan, RS
dc.contributor.authorHo, WL
dc.contributor.authorMak, GC
dc.contributor.authorTsang, KW
dc.contributor.authorYam, WC
dc.contributor.authorYuen, KY
dc.date.accessioned2012-08-08T08:49:44Z
dc.date.available2012-08-08T08:49:44Z
dc.date.issued2005
dc.description.abstractObjectives: A study was conducted to evaluate the occurrence and characterization of extended-spectrum β-lactamases (ESBLs) among blood isolates of Proteus mirabilis collected over a 4 year period in Hong Kong. Methods: Production of ESBLs among 99 consecutive and non-duplicate isolates was evaluated by the double-disc synergy test. The ESBLs were characterized by isoelectric focusing and PCR sequencing using specific primers. The epidemiological relationship of the isolates was studied by the Dienes test and PFGE. Results: ESBLs were identified in 13 isolates, from none in 1999-2000 and up to 18.5% (5/27) in 2001 and 25.8% (8/31) in 2002. The ESBL-producing isolates were more resistant to ceftriaxone than to ceftazidime, and were more likely than non-ESBL-producers to have resistance to ciprofloxacin (76.9% versus 14%) and gentamicin (38.5% versus 9.3%). The ESBL content included CTX-M-13 (n=8), CTX-M-14 (n=3), SHV-5 (n=2), TEM-11 (n=1), and an unidentified ESBL with a pI of 7.5. The Dienes test revealed that the genetic background in the 99 isolates was highly heterogeneous, with 54 distinct types among 92 isolates and seven were non-typeable. Among the 13 ESBL-producing isolates, five different backgrounds, including one cluster (Dienes-pulsotype A) with nine isolates, were identified by both Dienes test and PFGE, thus suggesting both clonal and multi-clonal spread of the CTX-M enzymes. Conclusions: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition. © The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 6, p. 840-845 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dki135
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dki135
dc.identifier.epage845
dc.identifier.hkuros100336
dc.identifier.isiWOS:000230028100007
dc.identifier.issn0305-7453
2011 Impact Factor: 5.068
2011 SCImago Journal Rankings: 0.422
dc.identifier.issue6
dc.identifier.pmid15857942
dc.identifier.scopuseid_2-s2.0-21244460355
dc.identifier.spage840
dc.identifier.urihttp://hdl.handle.net/10722/157411
dc.identifier.volume55
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.
dc.subject.meshDrug Resistance, Bacterial - Genetics
dc.subject.meshHumans
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshPlasmids
dc.subject.meshProteus Mirabilis - Classification - Drug Effects - Enzymology
dc.subject.meshBeta-Lactamases - Biosynthesis - Genetics
dc.titleOccurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong