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Article: Occurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002
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TitleOccurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002
 
AuthorsHo, PL1
Ho, AYM1
Chow, KH1
Wong, RCW1
Duan, RS1
Ho, WL1
Mak, GC1
Tsang, KW1
Yam, WC1
Yuen, KY1
 
Issue Date2005
 
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
CitationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 6, p. 840-845 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dki135
 
AbstractObjectives: A study was conducted to evaluate the occurrence and characterization of extended-spectrum β-lactamases (ESBLs) among blood isolates of Proteus mirabilis collected over a 4 year period in Hong Kong. Methods: Production of ESBLs among 99 consecutive and non-duplicate isolates was evaluated by the double-disc synergy test. The ESBLs were characterized by isoelectric focusing and PCR sequencing using specific primers. The epidemiological relationship of the isolates was studied by the Dienes test and PFGE. Results: ESBLs were identified in 13 isolates, from none in 1999-2000 and up to 18.5% (5/27) in 2001 and 25.8% (8/31) in 2002. The ESBL-producing isolates were more resistant to ceftriaxone than to ceftazidime, and were more likely than non-ESBL-producers to have resistance to ciprofloxacin (76.9% versus 14%) and gentamicin (38.5% versus 9.3%). The ESBL content included CTX-M-13 (n=8), CTX-M-14 (n=3), SHV-5 (n=2), TEM-11 (n=1), and an unidentified ESBL with a pI of 7.5. The Dienes test revealed that the genetic background in the 99 isolates was highly heterogeneous, with 54 distinct types among 92 isolates and seven were non-typeable. Among the 13 ESBL-producing isolates, five different backgrounds, including one cluster (Dienes-pulsotype A) with nine isolates, were identified by both Dienes test and PFGE, thus suggesting both clonal and multi-clonal spread of the CTX-M enzymes. Conclusions: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition. © The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
 
ISSN0305-7453
2013 Impact Factor: 5.439
 
DOIhttp://dx.doi.org/10.1093/jac/dki135
 
ISI Accession Number IDWOS:000230028100007
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHo, PL
 
dc.contributor.authorHo, AYM
 
dc.contributor.authorChow, KH
 
dc.contributor.authorWong, RCW
 
dc.contributor.authorDuan, RS
 
dc.contributor.authorHo, WL
 
dc.contributor.authorMak, GC
 
dc.contributor.authorTsang, KW
 
dc.contributor.authorYam, WC
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2012-08-08T08:49:44Z
 
dc.date.available2012-08-08T08:49:44Z
 
dc.date.issued2005
 
dc.description.abstractObjectives: A study was conducted to evaluate the occurrence and characterization of extended-spectrum β-lactamases (ESBLs) among blood isolates of Proteus mirabilis collected over a 4 year period in Hong Kong. Methods: Production of ESBLs among 99 consecutive and non-duplicate isolates was evaluated by the double-disc synergy test. The ESBLs were characterized by isoelectric focusing and PCR sequencing using specific primers. The epidemiological relationship of the isolates was studied by the Dienes test and PFGE. Results: ESBLs were identified in 13 isolates, from none in 1999-2000 and up to 18.5% (5/27) in 2001 and 25.8% (8/31) in 2002. The ESBL-producing isolates were more resistant to ceftriaxone than to ceftazidime, and were more likely than non-ESBL-producers to have resistance to ciprofloxacin (76.9% versus 14%) and gentamicin (38.5% versus 9.3%). The ESBL content included CTX-M-13 (n=8), CTX-M-14 (n=3), SHV-5 (n=2), TEM-11 (n=1), and an unidentified ESBL with a pI of 7.5. The Dienes test revealed that the genetic background in the 99 isolates was highly heterogeneous, with 54 distinct types among 92 isolates and seven were non-typeable. Among the 13 ESBL-producing isolates, five different backgrounds, including one cluster (Dienes-pulsotype A) with nine isolates, were identified by both Dienes test and PFGE, thus suggesting both clonal and multi-clonal spread of the CTX-M enzymes. Conclusions: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition. © The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 6, p. 840-845 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dki135
 
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dki135
 
dc.identifier.epage845
 
dc.identifier.hkuros100336
 
dc.identifier.isiWOS:000230028100007
 
dc.identifier.issn0305-7453
2013 Impact Factor: 5.439
 
dc.identifier.issue6
 
dc.identifier.pmid15857942
 
dc.identifier.scopuseid_2-s2.0-21244460355
 
dc.identifier.spage840
 
dc.identifier.urihttp://hdl.handle.net/10722/157411
 
dc.identifier.volume55
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.
 
dc.subject.meshDrug Resistance, Bacterial - Genetics
 
dc.subject.meshHumans
 
dc.subject.meshMicrobial Sensitivity Tests
 
dc.subject.meshPlasmids
 
dc.subject.meshProteus Mirabilis - Classification - Drug Effects - Enzymology
 
dc.subject.meshBeta-Lactamases - Biosynthesis - Genetics
 
dc.titleOccurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002
 
dc.typeArticle
 
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<contributor.author>Duan, RS</contributor.author>
<contributor.author>Ho, WL</contributor.author>
<contributor.author>Mak, GC</contributor.author>
<contributor.author>Tsang, KW</contributor.author>
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Author Affiliations
  1. The University of Hong Kong