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Article: Occurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002

TitleOccurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002
Authors
Issue Date2005
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 6, p. 840-845 How to Cite?
Abstract
Objectives: A study was conducted to evaluate the occurrence and characterization of extended-spectrum β-lactamases (ESBLs) among blood isolates of Proteus mirabilis collected over a 4 year period in Hong Kong. Methods: Production of ESBLs among 99 consecutive and non-duplicate isolates was evaluated by the double-disc synergy test. The ESBLs were characterized by isoelectric focusing and PCR sequencing using specific primers. The epidemiological relationship of the isolates was studied by the Dienes test and PFGE. Results: ESBLs were identified in 13 isolates, from none in 1999-2000 and up to 18.5% (5/27) in 2001 and 25.8% (8/31) in 2002. The ESBL-producing isolates were more resistant to ceftriaxone than to ceftazidime, and were more likely than non-ESBL-producers to have resistance to ciprofloxacin (76.9% versus 14%) and gentamicin (38.5% versus 9.3%). The ESBL content included CTX-M-13 (n=8), CTX-M-14 (n=3), SHV-5 (n=2), TEM-11 (n=1), and an unidentified ESBL with a pI of 7.5. The Dienes test revealed that the genetic background in the 99 isolates was highly heterogeneous, with 54 distinct types among 92 isolates and seven were non-typeable. Among the 13 ESBL-producing isolates, five different backgrounds, including one cluster (Dienes-pulsotype A) with nine isolates, were identified by both Dienes test and PFGE, thus suggesting both clonal and multi-clonal spread of the CTX-M enzymes. Conclusions: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition. © The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157411
ISSN
2013 Impact Factor: 5.439
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorHo, PLen_US
dc.contributor.authorHo, AYMen_US
dc.contributor.authorChow, KHen_US
dc.contributor.authorWong, RCWen_US
dc.contributor.authorDuan, RSen_US
dc.contributor.authorHo, WLen_US
dc.contributor.authorMak, GCen_US
dc.contributor.authorTsang, KWen_US
dc.contributor.authorYam, WCen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:49:44Z-
dc.date.available2012-08-08T08:49:44Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 6, p. 840-845en_US
dc.identifier.issn0305-7453en_US
dc.identifier.urihttp://hdl.handle.net/10722/157411-
dc.description.abstractObjectives: A study was conducted to evaluate the occurrence and characterization of extended-spectrum β-lactamases (ESBLs) among blood isolates of Proteus mirabilis collected over a 4 year period in Hong Kong. Methods: Production of ESBLs among 99 consecutive and non-duplicate isolates was evaluated by the double-disc synergy test. The ESBLs were characterized by isoelectric focusing and PCR sequencing using specific primers. The epidemiological relationship of the isolates was studied by the Dienes test and PFGE. Results: ESBLs were identified in 13 isolates, from none in 1999-2000 and up to 18.5% (5/27) in 2001 and 25.8% (8/31) in 2002. The ESBL-producing isolates were more resistant to ceftriaxone than to ceftazidime, and were more likely than non-ESBL-producers to have resistance to ciprofloxacin (76.9% versus 14%) and gentamicin (38.5% versus 9.3%). The ESBL content included CTX-M-13 (n=8), CTX-M-14 (n=3), SHV-5 (n=2), TEM-11 (n=1), and an unidentified ESBL with a pI of 7.5. The Dienes test revealed that the genetic background in the 99 isolates was highly heterogeneous, with 54 distinct types among 92 isolates and seven were non-typeable. Among the 13 ESBL-producing isolates, five different backgrounds, including one cluster (Dienes-pulsotype A) with nine isolates, were identified by both Dienes test and PFGE, thus suggesting both clonal and multi-clonal spread of the CTX-M enzymes. Conclusions: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition. © The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_US
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_US
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.-
dc.subject.meshDrug Resistance, Bacterial - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshProteus Mirabilis - Classification - Drug Effects - Enzymologyen_US
dc.subject.meshBeta-Lactamases - Biosynthesis - Geneticsen_US
dc.titleOccurrence and molecular analysis of extended-spectrum β-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002en_US
dc.typeArticleen_US
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_US
dc.identifier.emailChow, KH:khchowb@hku.hken_US
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityHo, PL=rp00406en_US
dc.identifier.authorityChow, KH=rp00370en_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/jac/dki135en_US
dc.identifier.pmid15857942en_US
dc.identifier.scopuseid_2-s2.0-21244460355en_US
dc.identifier.hkuros100336-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21244460355&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume55en_US
dc.identifier.issue6en_US
dc.identifier.spage840en_US
dc.identifier.epage845en_US
dc.identifier.isiWOS:000230028100007-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridHo, AYM=35388387700en_US
dc.identifier.scopusauthoridChow, KH=7202180736en_US
dc.identifier.scopusauthoridWong, RCW=8612000100en_US
dc.identifier.scopusauthoridDuan, RS=8612000200en_US
dc.identifier.scopusauthoridHo, WL=36855641000en_US
dc.identifier.scopusauthoridMak, GC=8883252800en_US
dc.identifier.scopusauthoridTsang, KW=7201555024en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US

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