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Article: Host range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strain

TitleHost range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strain
Authors
Issue Date2005
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2005, v. 335 n. 2, p. 242-251 How to Cite?
AbstractVaccinia Tian Tan (VTT) was used as a vaccine against smallpox in China for millions of people before 1980, yet the biological characteristics of the virus remain unclear. We have characterized VTT with respect to its host cell range, growth properties in vitro, and virulence in vivo. We found that 11 of the 12 mammalian cell lines studied are permissive to VTT infection whereas one, CHO-K1, is non-permissive. Using electron microscopy and sequence analysis, we found that the restriction of VTT replication in CHO-K1 is at a step before viral maturation probably due to the loss of the V025 gene. Moreover, VTT is significantly less virulent than vaccinia WR but remains neurovirulent in mice and causes significant body weight loss after intranasal inoculation. Our data demonstrate the need for further attenuation of VTT to serve either as a safer smallpox vaccine or as a live vaccine vector for other pathogens. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157408
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFang, Qen_US
dc.contributor.authorYang, Len_US
dc.contributor.authorZhu, Wen_US
dc.contributor.authorLiu, Len_US
dc.contributor.authorWang, Hen_US
dc.contributor.authorYu, Wen_US
dc.contributor.authorXiao, Gen_US
dc.contributor.authorTien, Pen_US
dc.contributor.authorZhang, Len_US
dc.contributor.authorChen, Zen_US
dc.date.accessioned2012-08-08T08:49:43Z-
dc.date.available2012-08-08T08:49:43Z-
dc.date.issued2005en_US
dc.identifier.citationVirology, 2005, v. 335 n. 2, p. 242-251en_US
dc.identifier.issn0042-6822en_US
dc.identifier.urihttp://hdl.handle.net/10722/157408-
dc.description.abstractVaccinia Tian Tan (VTT) was used as a vaccine against smallpox in China for millions of people before 1980, yet the biological characteristics of the virus remain unclear. We have characterized VTT with respect to its host cell range, growth properties in vitro, and virulence in vivo. We found that 11 of the 12 mammalian cell lines studied are permissive to VTT infection whereas one, CHO-K1, is non-permissive. Using electron microscopy and sequence analysis, we found that the restriction of VTT replication in CHO-K1 is at a step before viral maturation probably due to the loss of the V025 gene. Moreover, VTT is significantly less virulent than vaccinia WR but remains neurovirulent in mice and causes significant body weight loss after intranasal inoculation. Our data demonstrate the need for further attenuation of VTT to serve either as a safer smallpox vaccine or as a live vaccine vector for other pathogens. © 2005 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_US
dc.relation.ispartofVirologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain - Virologyen_US
dc.subject.meshCytopathogenic Effect, Viralen_US
dc.subject.meshEpithelial Cells - Ultrastructure - Virologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFibroblasts - Ultrastructure - Virologyen_US
dc.subject.meshHumansen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshOrgan Specificityen_US
dc.subject.meshSmallpox Vaccineen_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshVaccinia - Physiopathology - Virologyen_US
dc.subject.meshVaccinia Virus - Classification - Growth & Development - Pathogenicity - Ultrastructureen_US
dc.subject.meshVirulenceen_US
dc.subject.meshVirus Assemblyen_US
dc.subject.meshVirus Replicationen_US
dc.subject.meshWeight Lossen_US
dc.titleHost range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strainen_US
dc.typeArticleen_US
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.virol.2005.02.014en_US
dc.identifier.pmid15840523-
dc.identifier.scopuseid_2-s2.0-20244371532en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20244371532&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume335en_US
dc.identifier.issue2en_US
dc.identifier.spage242en_US
dc.identifier.epage251en_US
dc.identifier.isiWOS:000228761600010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFang, Q=55248545600en_US
dc.identifier.scopusauthoridYang, L=7406278711en_US
dc.identifier.scopusauthoridZhu, W=8377176500en_US
dc.identifier.scopusauthoridLiu, L=36068379000en_US
dc.identifier.scopusauthoridWang, H=8724886600en_US
dc.identifier.scopusauthoridYu, W=50162901500en_US
dc.identifier.scopusauthoridXiao, G=8377176900en_US
dc.identifier.scopusauthoridTien, P=7006421622en_US
dc.identifier.scopusauthoridZhang, L=8783285300en_US
dc.identifier.scopusauthoridChen, Z=35271180800en_US

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