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Article: Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess

TitleAlkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess
Authors
Issue Date2005
PublisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/sam
Citation
Systematic And Applied Microbiology, 2005, v. 28 n. 4, p. 316-322 How to Cite?
AbstractA bacterium was isolated from the abscess pus of a 72-year-old patient with Warthin's tumor and parotid abscess. The cells were aerobic, non-motile, Gram-negative but difficult to be destained, non-sporulating, coccobacillus. The bacterium grew poorly on sheep blood agar and MacConkey agar as non-hemolytic colonies of 0.5 mm in diameter after 24 h of incubation at 37°C in ambient air. Growth was enhanced by Tween 80. It produces catalase but not cytochrome oxidase. Sequencing of the cloned 16S rRNA PCR products of the bacterium revealed three different 16S rRNA gene sequences, with 12-31 bp differences among them. Phylogenetic analysis showed that the bacterium is closely related to Alkanindiges illinoisensis, with 5.0-5.9% differences between the 16S rRNA gene sequence of the bacterium and that of A. illinoisensis. Tryptophan auxotrophic strain of Acinetobacter trpE27 transformed with DNA extracted from the bacterium was unable to grow on tryptophan deficient medium, indicating that the bacterium was not a strain of Acinetobacter. The G+C content of the bacterium (mean±SD) was 46.9±4.3%. A new species, Alkanindiges hongkongensis sp. nov., is proposed, for which HKU9T is the type strain. Isolates with "small colonies" that are apparently Acinetobacter-like species should be carefully identified. Growth enhancement with aliphatic hydrocarbons should be looked for and 16S rRNA gene sequencing performed in order to find more potential cases of Alkanindiges infections, as well as to define the epidemiology, clinical spectrum, and outcome of infections associated with this genus. © 2005 Elsevier GmbH. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157406
ISSN
2015 Impact Factor: 3.691
2015 SCImago Journal Rankings: 1.282
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_US
dc.contributor.authorTse, Hen_US
dc.contributor.authorLau, SKPen_US
dc.contributor.authorLeung, KWen_US
dc.contributor.authorWoo, GKSen_US
dc.contributor.authorWong, MKMen_US
dc.contributor.authorHo, CMen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:49:41Z-
dc.date.available2012-08-08T08:49:41Z-
dc.date.issued2005en_US
dc.identifier.citationSystematic And Applied Microbiology, 2005, v. 28 n. 4, p. 316-322en_US
dc.identifier.issn0723-2020en_US
dc.identifier.urihttp://hdl.handle.net/10722/157406-
dc.description.abstractA bacterium was isolated from the abscess pus of a 72-year-old patient with Warthin's tumor and parotid abscess. The cells were aerobic, non-motile, Gram-negative but difficult to be destained, non-sporulating, coccobacillus. The bacterium grew poorly on sheep blood agar and MacConkey agar as non-hemolytic colonies of 0.5 mm in diameter after 24 h of incubation at 37°C in ambient air. Growth was enhanced by Tween 80. It produces catalase but not cytochrome oxidase. Sequencing of the cloned 16S rRNA PCR products of the bacterium revealed three different 16S rRNA gene sequences, with 12-31 bp differences among them. Phylogenetic analysis showed that the bacterium is closely related to Alkanindiges illinoisensis, with 5.0-5.9% differences between the 16S rRNA gene sequence of the bacterium and that of A. illinoisensis. Tryptophan auxotrophic strain of Acinetobacter trpE27 transformed with DNA extracted from the bacterium was unable to grow on tryptophan deficient medium, indicating that the bacterium was not a strain of Acinetobacter. The G+C content of the bacterium (mean±SD) was 46.9±4.3%. A new species, Alkanindiges hongkongensis sp. nov., is proposed, for which HKU9T is the type strain. Isolates with "small colonies" that are apparently Acinetobacter-like species should be carefully identified. Growth enhancement with aliphatic hydrocarbons should be looked for and 16S rRNA gene sequencing performed in order to find more potential cases of Alkanindiges infections, as well as to define the epidemiology, clinical spectrum, and outcome of infections associated with this genus. © 2005 Elsevier GmbH. All rights reserved.en_US
dc.languageengen_US
dc.publisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/samen_US
dc.relation.ispartofSystematic and Applied Microbiologyen_US
dc.subject.meshAbscess - Microbiologyen_US
dc.subject.meshAcinetobacter - Geneticsen_US
dc.subject.meshAdenolymphoma - Complications - Microbiologyen_US
dc.subject.meshAerobiosisen_US
dc.subject.meshAgeden_US
dc.subject.meshBase Compositionen_US
dc.subject.meshDna, Bacterial - Chemistry - Isolation & Purificationen_US
dc.subject.meshDna, Ribosomal - Chemistry - Isolation & Purificationen_US
dc.subject.meshGenes, Rrnaen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMoraxellaceae - Classification - Cytology - Isolation & Purification - Physiologyen_US
dc.subject.meshMoraxellaceae Infections - Complications - Microbiologyen_US
dc.subject.meshParotid Diseases - Microbiologyen_US
dc.subject.meshParotid Gland - Microbiologyen_US
dc.subject.meshParotid Neoplasms - Complications - Microbiologyen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshRna, Ribosomal, 16S - Geneticsen_US
dc.subject.meshTransformation, Bacterialen_US
dc.titleAlkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscessen_US
dc.typeArticleen_US
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_US
dc.identifier.emailTse, H:herman@graduate.hku.hken_US
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWoo, PCY=rp00430en_US
dc.identifier.authorityTse, H=rp00519en_US
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.syapm.2005.01.003en_US
dc.identifier.pmid15997704-
dc.identifier.scopuseid_2-s2.0-19444385177en_US
dc.identifier.hkuros114673-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-19444385177&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume28en_US
dc.identifier.issue4en_US
dc.identifier.spage316en_US
dc.identifier.epage322en_US
dc.identifier.isiWOS:000230346200005-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridWoo, PCY=7201801340en_US
dc.identifier.scopusauthoridTse, H=7006070596en_US
dc.identifier.scopusauthoridLau, SKP=7401596211en_US
dc.identifier.scopusauthoridLeung, KW=7401860831en_US
dc.identifier.scopusauthoridWoo, GKS=7006485416en_US
dc.identifier.scopusauthoridWong, MKM=35123119500en_US
dc.identifier.scopusauthoridHo, CM=7404652540en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US

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