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Article: Detection and characterization of extended-spectrum β-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002
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TitleDetection and characterization of extended-spectrum β-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002
 
AuthorsHo, PL1
Shek, RHL1
Chow, KH1
Duan, RS1
Mak, GC1
Lai, EL1
Yam, WC1
Tsang, KW1
Lai, WM1
 
Issue Date2005
 
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
CitationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 3, p. 326-332 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dki010
 
AbstractObjectives: A total of 139 consecutive and non-duplicate bloodstream isolates of Enterobacter spp. collected from inpatients in Hong Kong during 2000-2002 were studied for production of extended-spectrum β-lactamases (ESBLs). Methods: All isolates were evaluated by the modified double-disc synergy test (m-DDST), the combined disc method (CDM) and the three-dimensional (3D) test. The m-DDST and CDM were modified by the use of cefepime discs. β-Lactamases were characterized by isoelectric focusing and PCR sequencing using specific primers. Results: ESBLs were identified in nine isolates (overall 6.5%), including seven of 39 (17.9%) Enterobacter hormaechei, one of 27 (3.7%) Enterobacter aerogenes and the only Enterobacter intermedius strain. The E. intermedius strain was positive only in the 3D test but not in the other two tests. The other eight strains were positive in all three tests. No ESBL was detected in the other species, including non-hormaechei members of the Enterobacter cloacae complex (n = 61), Enterobacter agglomerans (n = 7), Enterobacter gergoviae (n = 4) and Enterobacter sakazakii (n = 1). The ESBL content included five different CTX-M enzymes (CTX-M-9, CTX-M-13, CTX-M-14, CTX-M-24 and a novel CTX-M-2-like β-lactamase), SHV-12 (n = 2) and unidentifiable ESBLs with a pl of 7.7 or 7.9 in two strains. The seven ESBL-producing E. hormaechei were genotyped by pulsed-field gel electrophoresis and were found to be unrelated to each other. In three of the CTX-M-producing strains, ISEpp1-like elements, including promoters for the β-lactamase gene, were found. Conclusions: Our data underscore the diversity of CTX-M enzymes among Enterobacter spp. in Hong Kong. © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved.
 
ISSN0305-7453
2013 Impact Factor: 5.439
 
DOIhttp://dx.doi.org/10.1093/jac/dki010
 
ISI Accession Number IDWOS:000227404000009
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHo, PL
 
dc.contributor.authorShek, RHL
 
dc.contributor.authorChow, KH
 
dc.contributor.authorDuan, RS
 
dc.contributor.authorMak, GC
 
dc.contributor.authorLai, EL
 
dc.contributor.authorYam, WC
 
dc.contributor.authorTsang, KW
 
dc.contributor.authorLai, WM
 
dc.date.accessioned2012-08-08T08:49:37Z
 
dc.date.available2012-08-08T08:49:37Z
 
dc.date.issued2005
 
dc.description.abstractObjectives: A total of 139 consecutive and non-duplicate bloodstream isolates of Enterobacter spp. collected from inpatients in Hong Kong during 2000-2002 were studied for production of extended-spectrum β-lactamases (ESBLs). Methods: All isolates were evaluated by the modified double-disc synergy test (m-DDST), the combined disc method (CDM) and the three-dimensional (3D) test. The m-DDST and CDM were modified by the use of cefepime discs. β-Lactamases were characterized by isoelectric focusing and PCR sequencing using specific primers. Results: ESBLs were identified in nine isolates (overall 6.5%), including seven of 39 (17.9%) Enterobacter hormaechei, one of 27 (3.7%) Enterobacter aerogenes and the only Enterobacter intermedius strain. The E. intermedius strain was positive only in the 3D test but not in the other two tests. The other eight strains were positive in all three tests. No ESBL was detected in the other species, including non-hormaechei members of the Enterobacter cloacae complex (n = 61), Enterobacter agglomerans (n = 7), Enterobacter gergoviae (n = 4) and Enterobacter sakazakii (n = 1). The ESBL content included five different CTX-M enzymes (CTX-M-9, CTX-M-13, CTX-M-14, CTX-M-24 and a novel CTX-M-2-like β-lactamase), SHV-12 (n = 2) and unidentifiable ESBLs with a pl of 7.7 or 7.9 in two strains. The seven ESBL-producing E. hormaechei were genotyped by pulsed-field gel electrophoresis and were found to be unrelated to each other. In three of the CTX-M-producing strains, ISEpp1-like elements, including promoters for the β-lactamase gene, were found. Conclusions: Our data underscore the diversity of CTX-M enzymes among Enterobacter spp. in Hong Kong. © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 3, p. 326-332 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jac/dki010
 
dc.identifier.citeulike116917
 
dc.identifier.doihttp://dx.doi.org/10.1093/jac/dki010
 
dc.identifier.epage332
 
dc.identifier.hkuros106000
 
dc.identifier.isiWOS:000227404000009
 
dc.identifier.issn0305-7453
2013 Impact Factor: 5.439
 
dc.identifier.issue3
 
dc.identifier.pmid15681579
 
dc.identifier.scopuseid_2-s2.0-15844413964
 
dc.identifier.spage326
 
dc.identifier.urihttp://hdl.handle.net/10722/157398
 
dc.identifier.volume55
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.
 
dc.subject.meshBacteremia - Microbiology
 
dc.subject.meshEnterobacter - Enzymology
 
dc.subject.meshHong Kong
 
dc.subject.meshHumans
 
dc.subject.meshMicrobial Sensitivity Tests
 
dc.subject.meshTime Factors
 
dc.subject.meshBeta-Lactamases - Analysis - Genetics
 
dc.titleDetection and characterization of extended-spectrum β-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002
 
dc.typeArticle
 
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<contributor.author>Duan, RS</contributor.author>
<contributor.author>Mak, GC</contributor.author>
<contributor.author>Lai, EL</contributor.author>
<contributor.author>Yam, WC</contributor.author>
<contributor.author>Tsang, KW</contributor.author>
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Author Affiliations
  1. The University of Hong Kong