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Article: Detection and characterization of extended-spectrum β-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002

TitleDetection and characterization of extended-spectrum β-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002
Authors
Issue Date2005
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 3, p. 326-332 How to Cite?
AbstractObjectives: A total of 139 consecutive and non-duplicate bloodstream isolates of Enterobacter spp. collected from inpatients in Hong Kong during 2000-2002 were studied for production of extended-spectrum β-lactamases (ESBLs). Methods: All isolates were evaluated by the modified double-disc synergy test (m-DDST), the combined disc method (CDM) and the three-dimensional (3D) test. The m-DDST and CDM were modified by the use of cefepime discs. β-Lactamases were characterized by isoelectric focusing and PCR sequencing using specific primers. Results: ESBLs were identified in nine isolates (overall 6.5%), including seven of 39 (17.9%) Enterobacter hormaechei, one of 27 (3.7%) Enterobacter aerogenes and the only Enterobacter intermedius strain. The E. intermedius strain was positive only in the 3D test but not in the other two tests. The other eight strains were positive in all three tests. No ESBL was detected in the other species, including non-hormaechei members of the Enterobacter cloacae complex (n = 61), Enterobacter agglomerans (n = 7), Enterobacter gergoviae (n = 4) and Enterobacter sakazakii (n = 1). The ESBL content included five different CTX-M enzymes (CTX-M-9, CTX-M-13, CTX-M-14, CTX-M-24 and a novel CTX-M-2-like β-lactamase), SHV-12 (n = 2) and unidentifiable ESBLs with a pl of 7.7 or 7.9 in two strains. The seven ESBL-producing E. hormaechei were genotyped by pulsed-field gel electrophoresis and were found to be unrelated to each other. In three of the CTX-M-producing strains, ISEpp1-like elements, including promoters for the β-lactamase gene, were found. Conclusions: Our data underscore the diversity of CTX-M enzymes among Enterobacter spp. in Hong Kong. © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/157398
ISSN
2014 Impact Factor: 5.313
2014 SCImago Journal Rankings: 1.983
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_US
dc.contributor.authorShek, RHLen_US
dc.contributor.authorChow, KHen_US
dc.contributor.authorDuan, RSen_US
dc.contributor.authorMak, GCen_US
dc.contributor.authorLai, ELen_US
dc.contributor.authorYam, WCen_US
dc.contributor.authorTsang, KWen_US
dc.contributor.authorLai, WMen_US
dc.date.accessioned2012-08-08T08:49:37Z-
dc.date.available2012-08-08T08:49:37Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2005, v. 55 n. 3, p. 326-332en_US
dc.identifier.issn0305-7453en_US
dc.identifier.urihttp://hdl.handle.net/10722/157398-
dc.description.abstractObjectives: A total of 139 consecutive and non-duplicate bloodstream isolates of Enterobacter spp. collected from inpatients in Hong Kong during 2000-2002 were studied for production of extended-spectrum β-lactamases (ESBLs). Methods: All isolates were evaluated by the modified double-disc synergy test (m-DDST), the combined disc method (CDM) and the three-dimensional (3D) test. The m-DDST and CDM were modified by the use of cefepime discs. β-Lactamases were characterized by isoelectric focusing and PCR sequencing using specific primers. Results: ESBLs were identified in nine isolates (overall 6.5%), including seven of 39 (17.9%) Enterobacter hormaechei, one of 27 (3.7%) Enterobacter aerogenes and the only Enterobacter intermedius strain. The E. intermedius strain was positive only in the 3D test but not in the other two tests. The other eight strains were positive in all three tests. No ESBL was detected in the other species, including non-hormaechei members of the Enterobacter cloacae complex (n = 61), Enterobacter agglomerans (n = 7), Enterobacter gergoviae (n = 4) and Enterobacter sakazakii (n = 1). The ESBL content included five different CTX-M enzymes (CTX-M-9, CTX-M-13, CTX-M-14, CTX-M-24 and a novel CTX-M-2-like β-lactamase), SHV-12 (n = 2) and unidentifiable ESBLs with a pl of 7.7 or 7.9 in two strains. The seven ESBL-producing E. hormaechei were genotyped by pulsed-field gel electrophoresis and were found to be unrelated to each other. In three of the CTX-M-producing strains, ISEpp1-like elements, including promoters for the β-lactamase gene, were found. Conclusions: Our data underscore the diversity of CTX-M enzymes among Enterobacter spp. in Hong Kong. © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_US
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_US
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.-
dc.subject.meshBacteremia - Microbiologyen_US
dc.subject.meshEnterobacter - Enzymologyen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshBeta-Lactamases - Analysis - Geneticsen_US
dc.titleDetection and characterization of extended-spectrum β-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002en_US
dc.typeArticleen_US
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_US
dc.identifier.emailChow, KH:khchowb@hku.hken_US
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_US
dc.identifier.authorityHo, PL=rp00406en_US
dc.identifier.authorityChow, KH=rp00370en_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/jac/dki010en_US
dc.identifier.pmid15681579en_US
dc.identifier.scopuseid_2-s2.0-15844413964en_US
dc.identifier.hkuros106000-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15844413964&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume55en_US
dc.identifier.issue3en_US
dc.identifier.spage326en_US
dc.identifier.epage332en_US
dc.identifier.isiWOS:000227404000009-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridShek, RHL=8238476700en_US
dc.identifier.scopusauthoridChow, KH=7202180736en_US
dc.identifier.scopusauthoridDuan, RS=8612000200en_US
dc.identifier.scopusauthoridMak, GC=8883252800en_US
dc.identifier.scopusauthoridLai, EL=8238477100en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US
dc.identifier.scopusauthoridTsang, KW=7201555024en_US
dc.identifier.scopusauthoridLai, WM=35603486100en_US
dc.identifier.citeulike116917-

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