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Article: Broadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library
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TitleBroadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library
 
AuthorsZhang, MY6 3
Shu, Y6
Phogat, S6
Xiao, X6
Cham, F5
Bouma, P5
Choudhary, A5
Feng, YR5
Sanz, I4
Rybak, S1
Broder, CC5
Quinnan Jr, GV5
Evans, T2
Dimitrov, DS6
 
Issue Date2003
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
 
CitationJournal Of Immunological Methods, 2003, v. 283 n. 1-2, p. 17-25 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jim.2003.07.003
 
AbstractIdentification of broadly cross-reactive human monoclonal antibodies (mAbs) has major implications for development of vaccines, inhibitors and research tools. Here we describe a sequential antigen panning (SAP) methodology that may facilitate the selection of such antibodies. An HIV-specific antibody Fab (m18) was selected from a human Fab phage-display library by SAP against several recombinant soluble HIV envelope glycoproteins (Envs) and Env-sCD4 complexes. This Fab bound to a variety of recombinant soluble Envs (gp140s) from primary HIV isolates representing different clades, and inhibited cell fusion and virus entry mediated by Envs of primary HIV isolates. The methodology and the results may have implications for development of HIV vaccines and inhibitors, as well as for identification of antibodies to conserved epitopes on rapidly mutating viruses and cells.
 
ISSN0022-1759
2012 Impact Factor: 2.225
2012 SCImago Journal Rankings: 0.968
 
DOIhttp://dx.doi.org/10.1016/j.jim.2003.07.003
 
ISI Accession Number IDWOS:000187349900003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhang, MY
 
dc.contributor.authorShu, Y
 
dc.contributor.authorPhogat, S
 
dc.contributor.authorXiao, X
 
dc.contributor.authorCham, F
 
dc.contributor.authorBouma, P
 
dc.contributor.authorChoudhary, A
 
dc.contributor.authorFeng, YR
 
dc.contributor.authorSanz, I
 
dc.contributor.authorRybak, S
 
dc.contributor.authorBroder, CC
 
dc.contributor.authorQuinnan Jr, GV
 
dc.contributor.authorEvans, T
 
dc.contributor.authorDimitrov, DS
 
dc.date.accessioned2012-08-08T08:49:32Z
 
dc.date.available2012-08-08T08:49:32Z
 
dc.date.issued2003
 
dc.description.abstractIdentification of broadly cross-reactive human monoclonal antibodies (mAbs) has major implications for development of vaccines, inhibitors and research tools. Here we describe a sequential antigen panning (SAP) methodology that may facilitate the selection of such antibodies. An HIV-specific antibody Fab (m18) was selected from a human Fab phage-display library by SAP against several recombinant soluble HIV envelope glycoproteins (Envs) and Env-sCD4 complexes. This Fab bound to a variety of recombinant soluble Envs (gp140s) from primary HIV isolates representing different clades, and inhibited cell fusion and virus entry mediated by Envs of primary HIV isolates. The methodology and the results may have implications for development of HIV vaccines and inhibitors, as well as for identification of antibodies to conserved epitopes on rapidly mutating viruses and cells.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Immunological Methods, 2003, v. 283 n. 1-2, p. 17-25 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jim.2003.07.003
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jim.2003.07.003
 
dc.identifier.epage25
 
dc.identifier.isiWOS:000187349900003
 
dc.identifier.issn0022-1759
2012 Impact Factor: 2.225
2012 SCImago Journal Rankings: 0.968
 
dc.identifier.issue1-2
 
dc.identifier.pmid14659896
 
dc.identifier.scopuseid_2-s2.0-10744226328
 
dc.identifier.spage17
 
dc.identifier.urihttp://hdl.handle.net/10722/157385
 
dc.identifier.volume283
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Immunological Methods
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAntibodies, Monoclonal - Immunology
 
dc.subject.meshCross Reactions
 
dc.subject.meshGene Products, Env - Immunology
 
dc.subject.meshHiv Antibodies - Immunology
 
dc.subject.meshHiv Envelope Protein Gp120 - Immunology
 
dc.subject.meshHumans
 
dc.subject.meshImmunoglobulin Fab Fragments - Immunology
 
dc.subject.meshMembrane Fusion
 
dc.subject.meshPeptide Library
 
dc.subject.meshEnv Gene Products, Human Immunodeficiency Virus
 
dc.titleBroadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library
 
dc.typeArticle
 
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<contributor.author>Sanz, I</contributor.author>
<contributor.author>Rybak, S</contributor.author>
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<contributor.author>Quinnan Jr, GV</contributor.author>
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Author Affiliations
  1. National Cancer Institute at Frederick
  2. UC Davis
  3. SAIC-Frederick
  4. University of Rochester
  5. Uniformed Services University of the Health Sciences
  6. National Institutes of Health, Bethesda