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Article: Broadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library

TitleBroadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library
Authors
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
Citation
Journal Of Immunological Methods, 2003, v. 283 n. 1-2, p. 17-25 How to Cite?
AbstractIdentification of broadly cross-reactive human monoclonal antibodies (mAbs) has major implications for development of vaccines, inhibitors and research tools. Here we describe a sequential antigen panning (SAP) methodology that may facilitate the selection of such antibodies. An HIV-specific antibody Fab (m18) was selected from a human Fab phage-display library by SAP against several recombinant soluble HIV envelope glycoproteins (Envs) and Env-sCD4 complexes. This Fab bound to a variety of recombinant soluble Envs (gp140s) from primary HIV isolates representing different clades, and inhibited cell fusion and virus entry mediated by Envs of primary HIV isolates. The methodology and the results may have implications for development of HIV vaccines and inhibitors, as well as for identification of antibodies to conserved epitopes on rapidly mutating viruses and cells.
Persistent Identifierhttp://hdl.handle.net/10722/157385
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.053
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, MYen_US
dc.contributor.authorShu, Yen_US
dc.contributor.authorPhogat, Sen_US
dc.contributor.authorXiao, Xen_US
dc.contributor.authorCham, Fen_US
dc.contributor.authorBouma, Pen_US
dc.contributor.authorChoudhary, Aen_US
dc.contributor.authorFeng, YRen_US
dc.contributor.authorSanz, Ien_US
dc.contributor.authorRybak, Sen_US
dc.contributor.authorBroder, CCen_US
dc.contributor.authorQuinnan Jr, GVen_US
dc.contributor.authorEvans, Ten_US
dc.contributor.authorDimitrov, DSen_US
dc.date.accessioned2012-08-08T08:49:32Z-
dc.date.available2012-08-08T08:49:32Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Immunological Methods, 2003, v. 283 n. 1-2, p. 17-25en_US
dc.identifier.issn0022-1759en_US
dc.identifier.urihttp://hdl.handle.net/10722/157385-
dc.description.abstractIdentification of broadly cross-reactive human monoclonal antibodies (mAbs) has major implications for development of vaccines, inhibitors and research tools. Here we describe a sequential antigen panning (SAP) methodology that may facilitate the selection of such antibodies. An HIV-specific antibody Fab (m18) was selected from a human Fab phage-display library by SAP against several recombinant soluble HIV envelope glycoproteins (Envs) and Env-sCD4 complexes. This Fab bound to a variety of recombinant soluble Envs (gp140s) from primary HIV isolates representing different clades, and inhibited cell fusion and virus entry mediated by Envs of primary HIV isolates. The methodology and the results may have implications for development of HIV vaccines and inhibitors, as well as for identification of antibodies to conserved epitopes on rapidly mutating viruses and cells.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jimen_US
dc.relation.ispartofJournal of Immunological Methodsen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshCross Reactionsen_US
dc.subject.meshGene Products, Env - Immunologyen_US
dc.subject.meshHiv Antibodies - Immunologyen_US
dc.subject.meshHiv Envelope Protein Gp120 - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Fab Fragments - Immunologyen_US
dc.subject.meshMembrane Fusionen_US
dc.subject.meshPeptide Libraryen_US
dc.subject.meshEnv Gene Products, Human Immunodeficiency Virusen_US
dc.titleBroadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display libraryen_US
dc.typeArticleen_US
dc.identifier.emailZhang, MY:zhangmy@hku.hken_US
dc.identifier.authorityZhang, MY=rp01409en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jim.2003.07.003en_US
dc.identifier.pmid14659896-
dc.identifier.scopuseid_2-s2.0-10744226328en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10744226328&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume283en_US
dc.identifier.issue1-2en_US
dc.identifier.spage17en_US
dc.identifier.epage25en_US
dc.identifier.isiWOS:000187349900003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhang, MY=35316639300en_US
dc.identifier.scopusauthoridShu, Y=7103239429en_US
dc.identifier.scopusauthoridPhogat, S=6602665528en_US
dc.identifier.scopusauthoridXiao, X=7402168892en_US
dc.identifier.scopusauthoridCham, F=6507851729en_US
dc.identifier.scopusauthoridBouma, P=7003730370en_US
dc.identifier.scopusauthoridChoudhary, A=8743351100en_US
dc.identifier.scopusauthoridFeng, YR=7404543613en_US
dc.identifier.scopusauthoridSanz, I=7006277822en_US
dc.identifier.scopusauthoridRybak, S=35453517100en_US
dc.identifier.scopusauthoridBroder, CC=7004376461en_US
dc.identifier.scopusauthoridQuinnan Jr, GV=7006729933en_US
dc.identifier.scopusauthoridEvans, T=7401453852en_US
dc.identifier.scopusauthoridDimitrov, DS=7202564539en_US

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