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Article: Human Genital Epithelial Cells Capture Cell-Free Human Immunodeficiency Virus Type 1 and Transmit the Virus to CD4+ Cells: Implications for Mechanisms of Sexual Transmission

TitleHuman Genital Epithelial Cells Capture Cell-Free Human Immunodeficiency Virus Type 1 and Transmit the Virus to CD4+ Cells: Implications for Mechanisms of Sexual Transmission
Authors
Issue Date2003
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
Citation
Journal Of Infectious Diseases, 2003, v. 188 n. 10, p. 1473-1482 How to Cite?
AbstractSexual transmission of human immunodeficiency virus (HIV) accounts for the majority of new infections worldwide. However, the mechanism of viral transmission across the mucosal barrier is poorly understood. By use of an ectocervical epithelium-derived cell line, we found that the cells are capable of sequestering large amounts of HIV particles but are refractory to cell-free viral infection. The sequestered virus particles remained infectious for ≥6 days and resisted treatment with trypsin. Upon coculture with CD4 +-susceptible cells, epithelial cells can effectively transmit the virus to these cells, which can result in robust infection of the target cells. Inhibitory studies have shown that heparan sulfate moiety of cell-surface proteoglycans is involved in the viral attachment to these CD4-negative epithelial cells. Genital epithelial cells may play active roles in sequestering, protecting, and transferring virus during sexual transmission of HIV.
Persistent Identifierhttp://hdl.handle.net/10722/157380
ISSN
2015 Impact Factor: 6.344
2015 SCImago Journal Rankings: 4.000
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, Zen_US
dc.contributor.authorChen, Zen_US
dc.contributor.authorPhillips, DMen_US
dc.date.accessioned2012-08-08T08:49:29Z-
dc.date.available2012-08-08T08:49:29Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Infectious Diseases, 2003, v. 188 n. 10, p. 1473-1482en_US
dc.identifier.issn0022-1899en_US
dc.identifier.urihttp://hdl.handle.net/10722/157380-
dc.description.abstractSexual transmission of human immunodeficiency virus (HIV) accounts for the majority of new infections worldwide. However, the mechanism of viral transmission across the mucosal barrier is poorly understood. By use of an ectocervical epithelium-derived cell line, we found that the cells are capable of sequestering large amounts of HIV particles but are refractory to cell-free viral infection. The sequestered virus particles remained infectious for ≥6 days and resisted treatment with trypsin. Upon coculture with CD4 +-susceptible cells, epithelial cells can effectively transmit the virus to these cells, which can result in robust infection of the target cells. Inhibitory studies have shown that heparan sulfate moiety of cell-surface proteoglycans is involved in the viral attachment to these CD4-negative epithelial cells. Genital epithelial cells may play active roles in sequestering, protecting, and transferring virus during sexual transmission of HIV.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.orgen_US
dc.relation.ispartofJournal of Infectious Diseasesen_US
dc.subject.meshCd4-Positive T-Lymphocytes - Immunology - Virologyen_US
dc.subject.meshCervix Uteri - Immunology - Virologyen_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshDna, Viral - Chemistry - Geneticsen_US
dc.subject.meshEpithelial Cells - Virologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHiv Core Protein P24 - Immunologyen_US
dc.subject.meshHiv Infections - Immunology - Transmission - Virologyen_US
dc.subject.meshHiv-1en_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes, Mononuclear - Immunologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSexually Transmitted Diseases, Viral - Immunology - Transmission - Virologyen_US
dc.subject.meshViral Matrix Proteins - Immunologyen_US
dc.subject.meshVirus Replication - Immunologyen_US
dc.subject.meshZidovudine - Pharmacologyen_US
dc.titleHuman Genital Epithelial Cells Capture Cell-Free Human Immunodeficiency Virus Type 1 and Transmit the Virus to CD4+ Cells: Implications for Mechanisms of Sexual Transmissionen_US
dc.typeArticleen_US
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1086/379248en_US
dc.identifier.pmid14624372-
dc.identifier.scopuseid_2-s2.0-0346243730en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0346243730&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume188en_US
dc.identifier.issue10en_US
dc.identifier.spage1473en_US
dc.identifier.epage1482en_US
dc.identifier.isiWOS:000186681800009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWu, Z=16644120500en_US
dc.identifier.scopusauthoridChen, Z=35271180800en_US
dc.identifier.scopusauthoridPhillips, DM=7404518807en_US
dc.identifier.citeulike3801676-

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