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Article: Gradient and sensitivity enhanced multiple-quantum coherence in heteronuclear multidimensional NMR experiments

TitleGradient and sensitivity enhanced multiple-quantum coherence in heteronuclear multidimensional NMR experiments
Authors
KeywordsHmqc
Multidimensional Nmr
Protein
Sensitivity Enhancement
Issue Date1999
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-2738
Citation
Journal Of Biomolecular Nmr, 1999, v. 14 n. 2, p. 133-140 How to Cite?
AbstractRecent studies have indicated that the relaxation rate of the 1H- 13C multiple-quantum coherence is much slower than that of the 1H- 13C single- quantum coherence for non-aromatic methine sites in 13C labeled proteins and in nucleic acids at the slow tumbling limit. Several heteronuclear experiments have been designed to use a multiple-quantum coherence transfer scheme instead of the single-quantum transfer method, thereby increasing the sensitivity and resolution of the spectra. Here, we report a constant time, gradient and sensitivity enhanced HMQC experiment (CT-g/s-HMQC) and demonstrate that it has a significant sensitivity enhancement over constant time HMQC and constant time gradient and sensitivity enhanced HSQC experiments (CT-g/s-HSQC) when applied to a 13C and 15N labeled calmodulin sample in D 2O. We also apply this approach to 3D NOESY-HMQC and doubly sensitivity enhanced TOCSY-HMQC experiments, and demonstrate that they are more sensitive than their HSQC counterparts.
Persistent Identifierhttp://hdl.handle.net/10722/157376
ISSN
2015 Impact Factor: 3.439
2015 SCImago Journal Rankings: 2.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKong, XMen_US
dc.contributor.authorSze, KHen_US
dc.contributor.authorZhu, Gen_US
dc.date.accessioned2012-08-08T08:49:26Z-
dc.date.available2012-08-08T08:49:26Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Biomolecular Nmr, 1999, v. 14 n. 2, p. 133-140en_US
dc.identifier.issn0925-2738en_US
dc.identifier.urihttp://hdl.handle.net/10722/157376-
dc.description.abstractRecent studies have indicated that the relaxation rate of the 1H- 13C multiple-quantum coherence is much slower than that of the 1H- 13C single- quantum coherence for non-aromatic methine sites in 13C labeled proteins and in nucleic acids at the slow tumbling limit. Several heteronuclear experiments have been designed to use a multiple-quantum coherence transfer scheme instead of the single-quantum transfer method, thereby increasing the sensitivity and resolution of the spectra. Here, we report a constant time, gradient and sensitivity enhanced HMQC experiment (CT-g/s-HMQC) and demonstrate that it has a significant sensitivity enhancement over constant time HMQC and constant time gradient and sensitivity enhanced HSQC experiments (CT-g/s-HSQC) when applied to a 13C and 15N labeled calmodulin sample in D 2O. We also apply this approach to 3D NOESY-HMQC and doubly sensitivity enhanced TOCSY-HMQC experiments, and demonstrate that they are more sensitive than their HSQC counterparts.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-2738en_US
dc.relation.ispartofJournal of Biomolecular NMRen_US
dc.subjectHmqcen_US
dc.subjectMultidimensional Nmren_US
dc.subjectProteinen_US
dc.subjectSensitivity Enhancementen_US
dc.titleGradient and sensitivity enhanced multiple-quantum coherence in heteronuclear multidimensional NMR experimentsen_US
dc.typeArticleen_US
dc.identifier.emailSze, KH:khsze@hku.hken_US
dc.identifier.authoritySze, KH=rp00785en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/A:1004583910874en_US
dc.identifier.scopuseid_2-s2.0-0344417886en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344417886&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume14en_US
dc.identifier.issue2en_US
dc.identifier.spage133en_US
dc.identifier.epage140en_US
dc.identifier.isiWOS:000081300600004-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridKong, XM=7202794462en_US
dc.identifier.scopusauthoridSze, KH=7006735061en_US
dc.identifier.scopusauthoridZhu, G=7402633110en_US

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