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Article: Gradient and sensitivity enhancement of 2D TROSY with water flip-back, 3D NOESY-TROSY and TOCSY-TROSY experiments

TitleGradient and sensitivity enhancement of 2D TROSY with water flip-back, 3D NOESY-TROSY and TOCSY-TROSY experiments
Authors
KeywordsMultidimensional Nmr
Noesy-Trosy
Proteins
Sensitivity Enhancement
Tocsy- Trosy
Trosy
Water Flip-Back
Issue Date1999
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-2738
Citation
Journal Of Biomolecular Nmr, 1999, v. 13 n. 1, p. 77-81 How to Cite?
AbstractPreviously we demonstrated a sensitivity enhancement of the original TROSY experiment by a factor of √2 by the use of the sensitivity enhanced TROSY (en-TROSY) scheme. Here, we develop a gradient and sensitivity enhanced TROSY experiment (gs-TROSY), which is designed to select magnetization transfer pathways that suppress spectral artifacts and reduce the number of required phase cycles while having minimal loss of sensitivity. Both of these experimental methods (en-TROSY and gs-TROSY) have been combined with a water flip-back scheme which provides a further increase in sensitivity for labile NH groups by avoiding water saturation. We also apply these TROSY schemes to 3D NOESY-TROSY and 3D TOCSY-TROSY experiments.
Persistent Identifierhttp://hdl.handle.net/10722/157373
ISSN
2015 Impact Factor: 3.439
2015 SCImago Journal Rankings: 2.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, Gen_US
dc.contributor.authorKong, XMen_US
dc.contributor.authorSze, KHen_US
dc.date.accessioned2012-08-08T08:49:25Z-
dc.date.available2012-08-08T08:49:25Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Biomolecular Nmr, 1999, v. 13 n. 1, p. 77-81en_US
dc.identifier.issn0925-2738en_US
dc.identifier.urihttp://hdl.handle.net/10722/157373-
dc.description.abstractPreviously we demonstrated a sensitivity enhancement of the original TROSY experiment by a factor of √2 by the use of the sensitivity enhanced TROSY (en-TROSY) scheme. Here, we develop a gradient and sensitivity enhanced TROSY experiment (gs-TROSY), which is designed to select magnetization transfer pathways that suppress spectral artifacts and reduce the number of required phase cycles while having minimal loss of sensitivity. Both of these experimental methods (en-TROSY and gs-TROSY) have been combined with a water flip-back scheme which provides a further increase in sensitivity for labile NH groups by avoiding water saturation. We also apply these TROSY schemes to 3D NOESY-TROSY and 3D TOCSY-TROSY experiments.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-2738en_US
dc.relation.ispartofJournal of Biomolecular NMRen_US
dc.subjectMultidimensional Nmren_US
dc.subjectNoesy-Trosyen_US
dc.subjectProteinsen_US
dc.subjectSensitivity Enhancementen_US
dc.subjectTocsy- Trosyen_US
dc.subjectTrosyen_US
dc.subjectWater Flip-Backen_US
dc.titleGradient and sensitivity enhancement of 2D TROSY with water flip-back, 3D NOESY-TROSY and TOCSY-TROSY experimentsen_US
dc.typeArticleen_US
dc.identifier.emailSze, KH:khsze@hku.hken_US
dc.identifier.authoritySze, KH=rp00785en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/A:1008398227519en_US
dc.identifier.scopuseid_2-s2.0-0342326257en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0342326257&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue1en_US
dc.identifier.spage77en_US
dc.identifier.epage81en_US
dc.identifier.isiWOS:000078802400010-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhu, G=7402633110en_US
dc.identifier.scopusauthoridKong, XM=7202794462en_US
dc.identifier.scopusauthoridSze, KH=7006735061en_US

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