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Article: Characterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime

TitleCharacterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime
Authors
Issue Date2002
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal Of Antimicrobial Chemotherapy, 2002, v. 50 n. 5, p. 723-726 How to Cite?
AbstractBurkholderia pseudomallei produces an Ambler class A β-lactamase, known as BPS-1. The β-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of B. pseudomallei (LH-1-2) was cloned and expressed in Escherichia coli. The β-lactamase, named BPS-1m, had an identical isoelectric focusing point (pl 7.7) to that of BPS-1, but differed in having a stronger hydrolytic activity against ceftazidime. Susceptibility testing showed that BPS-1m when expressed in E. coli conferred resistance to ceftazidime (MIC ≥ 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop.
Persistent Identifierhttp://hdl.handle.net/10722/157344
ISSN
2015 Impact Factor: 4.919
2015 SCImago Journal Rankings: 2.157
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_US
dc.contributor.authorCheung, TKMen_US
dc.contributor.authorYam, WCen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:49:04Z-
dc.date.available2012-08-08T08:49:04Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2002, v. 50 n. 5, p. 723-726en_US
dc.identifier.issn0305-7453en_US
dc.identifier.urihttp://hdl.handle.net/10722/157344-
dc.description.abstractBurkholderia pseudomallei produces an Ambler class A β-lactamase, known as BPS-1. The β-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of B. pseudomallei (LH-1-2) was cloned and expressed in Escherichia coli. The β-lactamase, named BPS-1m, had an identical isoelectric focusing point (pl 7.7) to that of BPS-1, but differed in having a stronger hydrolytic activity against ceftazidime. Susceptibility testing showed that BPS-1m when expressed in E. coli conferred resistance to ceftazidime (MIC ≥ 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_US
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_US
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.-
dc.subject.meshAmino Acid Substitution - Geneticsen_US
dc.subject.meshBurkholderia Pseudomallei - Enzymology - Genetics - Isolation & Purificationen_US
dc.subject.meshCeftazidime - Metabolismen_US
dc.subject.meshGenetic Variationen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrolysisen_US
dc.subject.meshBeta-Lactam Resistance - Geneticsen_US
dc.subject.meshBeta-Lactamases - Genetics - Metabolismen_US
dc.titleCharacterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidimeen_US
dc.typeArticleen_US
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_US
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityHo, PL=rp00406en_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12407130-
dc.identifier.scopuseid_2-s2.0-0036849235en_US
dc.identifier.hkuros83455-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036849235&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume50en_US
dc.identifier.issue5en_US
dc.identifier.spage723en_US
dc.identifier.epage726en_US
dc.identifier.isiWOS:000179034900015-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHo, PL=7402211363en_US
dc.identifier.scopusauthoridCheung, TKM=7103334170en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US

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