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Article: CD4+ lymphocytopenia in acute infection of Asian macaques by a vaginally transmissible subtype-C, CCR5-tropic simian/human immunodeficiency virus (SHIV)

TitleCD4+ lymphocytopenia in acute infection of Asian macaques by a vaginally transmissible subtype-C, CCR5-tropic simian/human immunodeficiency virus (SHIV)
Authors
Issue Date2002
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jaids.com
Citation
Journal Of Acquired Immune Deficiency Syndromes, 2002, v. 30 n. 2, p. 133-145 How to Cite?
AbstractAn R5-tropic SHIVCHN19P4 was previously generated using a primary HIV-1 subtype-C envelope. We have further characterized this SHIV in two species of macaques. To determine whether this isolate is transmissible vaginally, female pigtailed macaques were inoculated with 2x103 TCID50 of SHIVCHN19P4 by the vaginal route. Animals became infected with a high peak plasma viremia (>107 viral copies/mL) and rapid seroconversion. The viremia was accompanied by CD4+ lymphocytopenia in the gut lamina propria lymphocyte (LPL) population. Comparable CD4+ T-cell loss was not seen in peripheral blood and colonic lymph nodes. These findings demonstrate a unique R5-tropic SHIV that can be used to study envelope-related issues in vaginal transmission of the most prevalent subtype of HIV-1. We also found that rhesus macaques intravenously inoculated with I x 103 TCID50 of SHIVCHN19P4 became infected and showed CD4+ lymphocytopenia in the gut LPL population. Despite inactivation of the vpu gene in SHIVCHN19P4, the virus appears to target mainly gut-associated lymphoid tissues during the initial stage of infection as has been described for SHIVSF162P, another R5-tropic (subtype B) recombinant virus. Our data indicate that the R5-mediated CD4+ lymphocytopenia in the gut is likely independent of HIV-1 genotypes and of the function of vpu at the acute phase of viral infection.
Persistent Identifierhttp://hdl.handle.net/10722/157340
ISSN
2015 Impact Factor: 3.806
2015 SCImago Journal Rankings: 2.434
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Zen_US
dc.contributor.authorZhao, Xen_US
dc.contributor.authorHuang, Yen_US
dc.contributor.authorGettie, Aen_US
dc.contributor.authorBa, Len_US
dc.contributor.authorBlanchard, Jen_US
dc.contributor.authorHo, DDen_US
dc.date.accessioned2012-08-08T08:49:03Z-
dc.date.available2012-08-08T08:49:03Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Acquired Immune Deficiency Syndromes, 2002, v. 30 n. 2, p. 133-145en_US
dc.identifier.issn1525-4135en_US
dc.identifier.urihttp://hdl.handle.net/10722/157340-
dc.description.abstractAn R5-tropic SHIVCHN19P4 was previously generated using a primary HIV-1 subtype-C envelope. We have further characterized this SHIV in two species of macaques. To determine whether this isolate is transmissible vaginally, female pigtailed macaques were inoculated with 2x103 TCID50 of SHIVCHN19P4 by the vaginal route. Animals became infected with a high peak plasma viremia (>107 viral copies/mL) and rapid seroconversion. The viremia was accompanied by CD4+ lymphocytopenia in the gut lamina propria lymphocyte (LPL) population. Comparable CD4+ T-cell loss was not seen in peripheral blood and colonic lymph nodes. These findings demonstrate a unique R5-tropic SHIV that can be used to study envelope-related issues in vaginal transmission of the most prevalent subtype of HIV-1. We also found that rhesus macaques intravenously inoculated with I x 103 TCID50 of SHIVCHN19P4 became infected and showed CD4+ lymphocytopenia in the gut LPL population. Despite inactivation of the vpu gene in SHIVCHN19P4, the virus appears to target mainly gut-associated lymphoid tissues during the initial stage of infection as has been described for SHIVSF162P, another R5-tropic (subtype B) recombinant virus. Our data indicate that the R5-mediated CD4+ lymphocytopenia in the gut is likely independent of HIV-1 genotypes and of the function of vpu at the acute phase of viral infection.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jaids.comen_US
dc.relation.ispartofJournal of Acquired Immune Deficiency Syndromesen_US
dc.subject.meshAcute Diseaseen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCd4-Positive T-Lymphocytes - Immunologyen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, Enven_US
dc.subject.meshGenes, Vpuen_US
dc.subject.meshHiv Infections - Immunology - Physiopathology - Transmission - Virologyen_US
dc.subject.meshHiv-1 - Classification - Metabolism - Pathogenicityen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphopenia - Immunology - Virologyen_US
dc.subject.meshMacaca Mulattaen_US
dc.subject.meshMacaca Nemestrinaen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshReceptors, Ccr5 - Metabolismen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSimian Acquired Immunodeficiency Syndrome - Immunology - Physiopathology - Transmission - Virologyen_US
dc.subject.meshSimian Immunodeficiency Virus - Classification - Metabolism - Pathogenicityen_US
dc.subject.meshVagina - Virologyen_US
dc.titleCD4+ lymphocytopenia in acute infection of Asian macaques by a vaginally transmissible subtype-C, CCR5-tropic simian/human immunodeficiency virus (SHIV)en_US
dc.typeArticleen_US
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12045675-
dc.identifier.scopuseid_2-s2.0-0036603368en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036603368&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume30en_US
dc.identifier.issue2en_US
dc.identifier.spage133en_US
dc.identifier.epage145en_US
dc.identifier.isiWOS:000176913400001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChen, Z=35271180800en_US
dc.identifier.scopusauthoridZhao, X=8781942500en_US
dc.identifier.scopusauthoridHuang, Y=7501575029en_US
dc.identifier.scopusauthoridGettie, A=7003730114en_US
dc.identifier.scopusauthoridBa, L=8557032300en_US
dc.identifier.scopusauthoridBlanchard, J=7202918172en_US
dc.identifier.scopusauthoridHo, DD=7402971998en_US

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